高产右旋糖苷酶微生物的筛选及产酶条件的优化

CN 41-1437/TS ISSN 2096-1553

高产右旋糖苷酶微生物的筛选及产酶条件的优化

黄丽 , 孟继坤 , 周嫄 , 李树波 , 徐羽

黄丽, 孟继坤, 周嫄, 等. 高产右旋糖苷酶微生物的筛选及产酶条件的优化[J]. 轻工学报, 2019, 34(1): 11-20. doi: 10.3969/j.issn.2096-1553.2019.01.002

引用本文: 黄丽, 孟继坤, 周嫄, 等. 高产右旋糖苷酶微生物的筛选及产酶条件的优化[J]. 轻工学报, 2019, 34(1): 11-20. doi: 10.3969/j.issn.2096-1553.2019.01.002

HUANG Li, MENG Jikun, ZHOU Yuan, et al. Screening of high yield dextran microorganisms and optimization of enzyme production conditions[J]. Journal of Light Industry, 2019, 34(1): 11-20. doi: 10.3969/j.issn.2096-1553.2019.01.002

Citation: HUANG Li, MENG Jikun, ZHOU Yuan, et al. Screening of high yield dextran microorganisms and optimization of enzyme production conditions[J]. Journal of Light Industry, 2019, 34(1): 11-20. doi: 10.3969/j.issn.2096-1553.2019.01.002

高产右旋糖苷酶微生物的筛选及产酶条件的优化

广西大学轻工与食品工程学院, 广西南宁 530000

作者简介: 黄丽(1997-),女,广西壮族自治区田阳县人,广西大学硕士研究生,主要研究方向为食品微生物工程.;

中图分类号: TS241

Screening of high yield dextran microorganisms and optimization of enzyme production conditions

School of Light Industry and Food Engineering, Guangxi University, Nanning 530000, China
Received Date: 2018-10-16

CLC number: TS241

摘要: 使用营养缺陷型培养基、运用透明圈法从甘蔗土壤中筛得高产右旋糖苷酶的微生物SGC-2，根据菌株的形态和16S rDNA基因序列对其进行分析鉴定，并对其发酵产酶条件进行优化.结果表明，SGC-2为巨大芽孢杆菌（Bacillus megatherium），该菌株的适宜产酶条件为：右旋糖酐T2000质量浓度20 g/L，牛肉膏质量浓度10 g/L，NaCl质量浓度4 g/L，MgSO₄质量浓度0.4 g/L，接种时间36 h，接种量3%，发酵时间96 h.该条件下，右旋糖苷酶酶活可高达28.32 U/mL，比优化前提高了151%.该右旋糖苷酶产生菌为首次报道，这为筛选高产右旋糖苷酶微生物的研究提供了一定的理论基础.
- 右旋糖酐 /
- 右旋糖苷酶 /
- 巨大芽孢杆菌 /
- 发酵优化
Abstract: The microorganism SGC-2 with high yield of dextran glycosidase was screened from sugarcane soil using nutrient-deficient medium and transparent circle method. The microorganism SGC-2 was identified according to its morphology and 16S rDNA gene sequence analysis. Moreover, the conditions of fermentation and enzyme production were further optimized. The results showed that the microorganism SGC-2 was Bacillus megatherium. The optimum conditions for enzyme production were as follows: dextran T2000 20 g/L, beef extract 10 g/L, NaCl 4 g/L, MgSO₄ 0.4 g/L, inoculation time 36 h, inoculation volume 3%, fermentation time 96 h. Under this condition, the activity of dextran anhydrase reached 28.32 U/mL, which increased by 151% when compared with that before optimization. The dextran anhydrase-producing strain was firstly reported, which provided a theoretical basis for screening high-yield dextran microorganisms.
- Polymer dextran /
- dextran /
- Bacillus megatherium /
- fermentation optimization
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沈阳化工大学材料科学与工程学院沈阳 110142

黄丽, 孟继坤, 周嫄, 等. 高产右旋糖苷酶微生物的筛选及产酶条件的优化[J]. 轻工学报, 2019, 34(1): 11-20. doi: 10.3969/j.issn.2096-1553.2019.01.002

引用本文: 黄丽, 孟继坤, 周嫄, 等. 高产右旋糖苷酶微生物的筛选及产酶条件的优化[J]. 轻工学报, 2019, 34(1): 11-20. doi: 10.3969/j.issn.2096-1553.2019.01.002

HUANG Li, MENG Jikun, ZHOU Yuan, et al. Screening of high yield dextran microorganisms and optimization of enzyme production conditions[J]. Journal of Light Industry, 2019, 34(1): 11-20. doi: 10.3969/j.issn.2096-1553.2019.01.002

Citation: HUANG Li, MENG Jikun, ZHOU Yuan, et al. Screening of high yield dextran microorganisms and optimization of enzyme production conditions[J]. Journal of Light Industry, 2019, 34(1): 11-20. doi: 10.3969/j.issn.2096-1553.2019.01.002

高产右旋糖苷酶微生物的筛选及产酶条件的优化

作者简介:黄丽(1997-),女,广西壮族自治区田阳县人,广西大学硕士研究生,主要研究方向为食品微生物工程.

广西大学轻工与食品工程学院, 广西南宁 530000

收稿日期: 2018-10-16

关键词:

摘要: 使用营养缺陷型培养基、运用透明圈法从甘蔗土壤中筛得高产右旋糖苷酶的微生物SGC-2，根据菌株的形态和16S rDNA基因序列对其进行分析鉴定，并对其发酵产酶条件进行优化.结果表明，SGC-2为巨大芽孢杆菌（Bacillus megatherium），该菌株的适宜产酶条件为：右旋糖酐T2000质量浓度20 g/L，牛肉膏质量浓度10 g/L，NaCl质量浓度4 g/L，MgSO₄质量浓度0.4 g/L，接种时间36 h，接种量3%，发酵时间96 h.该条件下，右旋糖苷酶酶活可高达28.32 U/mL，比优化前提高了151%.该右旋糖苷酶产生菌为首次报道，这为筛选高产右旋糖苷酶微生物的研究提供了一定的理论基础.

English Abstract

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