微紫青霉果胶酶降解再造烟叶高浓混合浆中果胶的研究
Study on the pectin from high-concentrated mixed slurry of reconstituted tobacco degraded by pectinase of Penicillium janthinellum
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摘要: 为降低再造烟叶中的果胶含量，采用单因素试验和正交试验对微紫青霉产果胶酶降解果胶的工艺条件进行优化，分析了酶解前后样品的热裂解产物，并将酶解后样品抄造制丝添加至卷烟中进行感官评价，结果表明：微紫青霉产果胶酶降解果胶的最佳反应条件为酶活力3000 U/mL，高浓混合浆干重与粗酶液体积的料液比1：1，反应温度50℃，处理时间3 h.在此条件下果胶降解率达到最大，为28.63%，相对分子量由341 kDa减小到55 kDa.酶解后样品裂解产物的组成和含量发生明显变化，除醇类物质外，其他香味物质酚类、醛酮类、酯类、杂环类减少.将酶解后的样品抄造制丝添加于卷烟中，香气质丰满细腻，透发性、甜润感增强，刺激性减小，余味纯净舒适，吸食品质得以改善，但劲头略有不足.Abstract: In order to reduce the content of pectin in reconstituted tobacco, the degradation process of pectin by pectinase of Penicillium janthinellum was optimized by single factor method and orthogonal design. Then the pyrolysis products of samples prior to and after enzymatic hydrolysis were analyzed.Moreover, the enzymatic hydrolysis samples were added to the cigarette for sensory evaluation. The results showed that the optimum conditions for pectin degradation by pectinase of penicillum janthinellum were as follows:enzyme activity 3000 U/mL, the ratio of liquid to solid 1:1, reaction temperature 50℃, treatment time 3 h. Under this condition, the degradation rate of pectin reached 28.63%.After enzymolysis, the molecular weight of pectin reduced from 341 kDa to 55 kDa. The composition and content of pyrolysis products changed obviously.Besides alcohols substance, other flavor components decreased such as phenols, aldehydes and ketones, esters and heterocyclic.When the enzymatic hydrolysis samples were added to the cigarette, the aroma of cigarette sucking was rich and delicate, the penetration and sweetness increased, the irritation decreased and the aftertaste was pure and comfortable, thereby improving the quality of cigarette sucking with slightly weak strength.
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Key words:
- Penicillium janthinellum /
- pectinase /
- reconstituted tobacco /
- pectin degradation rate
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[1]
刘蓓.微生物发酵降解造纸法烟草薄片有害前体物的研究[D].广州:华南理工大学,2013:53-54.
-
[2]
刘耀飞,常纪恒,于川芳.烟梗中果胶的酶法降解[J].烟草科技,2013(8):40.
-
[3]
KAR F, ARSLAN N. Effect of temperature and concentration on viscosity of orange peel pectin solutions and intrinsic viscosity-molecular weight relationship[J]. Carbohydrate Polymers,1999, 40:277.
-
[4]
王月侠,葛善礼,贾涛.烟梗化学组成的分析[J].烟草科技,1996(3):16.
-
[5]
闫克玉.烟草化学[M].郑州:郑州大学出版社,2002.
-
[6]
吴玉萍,杨光宇,王东丹.高效液相色谱法测定烟草中的果胶含量[J].光谱实验室,2004,21:183.
-
[7]
施林燕.微生物发酵及酶解烟梗物料的研究[D].无锡:江南大学:2012:37-38.
-
[8]
于建军,马海燕,杨寒文,等.利用果胶酶降解烟叶中果胶的研究[J].江西农业学报,2009(3):136.
-
[9]
于兴伟,许赣荣,汤朝起.黑曲霉固态发酵及酶解烟梗条件的研究[J].食品工业科技,2012,24(24):195.
-
[10]
马东萍,卫青,周谨,等.一种再造烟叶改性添加剂及其制备和使用方法:1947606A[P].2006-10-23.
-
[11]
柏婷.造纸法再造烟叶果胶降解技术研究[D].昆明:昆明理工大学,2014.
-
[12]
许春平,郝辉,孙斯文,等.果胶酶高产菌微紫青霉SW09的鉴定及发酵特征[J].湖北农业科学,2016,55(2):337.
-
[13]
郝辉,陈芝飞,宋金勇,等.微紫青霉(Penicillium janthinellum sw09)发酵产果胶酶降解烟梗果胶的条件优化及产物分析[J].西南农业学报,2015,28(6):2756.
-
[14]
马宇平.微紫青霉(Penicillium janthinellum sw09)发酵产果胶酶降解烟梗果胶产物对比分析[C]//中国化学会、国家自然科学基金委员会.中国化学会第二届全国质谱分析学术报告会会议摘要集.北京:中国化学会、国家自然科学基金委员会,2015:1.
-
[15]
郝辉,李萌姗,张展,等.果胶酶降解混合浆果胶的条件优化及热裂解分析[J].湖北农业科学,2016,55(3):710.
-
[16]
刘战民,陆兆新,吕凤霞,等.毕赤酵母工程菌原果胶酶的分离纯化[J].西北农林科技大学学报(自然科学版),2006(1):79.
-
[17]
王天龙,仇宏伟,陈海华,等.3,5-二硝基水杨酸法测定果胶酶活力的条件研究[J].食品与机械,2008,36(3):96.
-
[18]
刘玉佳,孔繁东,刘兆芳,等.桔梗多糖Sevag法除蛋白工艺的研究[J].中国调味品,2014(4):5.
-
[19]
柯斧,孙莹莹.秦山巴区野生豆腐木叶片中果胶含量的测定[J].陕西农业科学,2013(5):36.
-
[20]
范海涛,张虎成,曲伟,等.结合换算因子的苯酚-硫酸法测定红茶菌培养液中水溶性多糖含量的研究[J].天然产物研究与开发,2014(26):715.
-
[21]
国家烟草专卖局科科技教育司.GB5606-2005《卷烟》系列国家标准宣贯教材[M].北京:中国标准出版社,2005.
-
[22]
杨慧芳.降解烟梗果胶质微生物筛选及产果胶酶的研究[D].无锡:江南大学,2012:41-42.
-
[23]
李晓亮,郑赛晶,王志华,等.烟草及其主要组分的热分析质谱研究[J].化学研究与应用,2013,25(5):670.
-
[24]
胡永华,宁敏,张晓宇,等.不同热失重阶段烟草的裂解产物[J].烟草科技,2015,48(3):66.
-
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