白腐菌液体发酵降解烟梗木质素的研究
Study on the degradation of lignin in tobacco stem by liquid fermentation with white rot fungi
-
摘要: 以白腐菌P. chrysosporium为对象,采用连续传代驯化的方法提升菌体对烟碱的耐受度.通过比较人工陈化与液体发酵对烟梗中木质素的降解效率,并对其发酵条件进行优化.结果表明,驯化后的白腐菌P. chrysosporium可有效定植于烟梗上,在34℃,接种量6%的最适发酵条件下液体发酵24 h,烟梗中木质素含量为1.93%,降解了23.11%,与人工陈化3个月烟梗木质素降解情况相当,总糖和葡萄糖含量分别增加10.84%和4.86%,在一定程度上消除了蛋白质燃烧产生的不良气味,改善了烟梗燃烧时产生的木质气.Abstract: The white rot fungus (P.Chrysosporium) was subjected to continuous subcultivation and domestication to improve the nicotine tolerance of the fungus. The degradation efficiency of lignin in tobacco stem was compared between artificial aging and liquid fermentation to obtain the optimal fermentation conditions. The results showed that the domesticated white rot fungi was effectively planted on tobacco stems. The content of lignin in tobacco stems reached 1.93%, decreasing by 23.11% after 24 h of liquid fermentation under the optimum fermentation conditions of 34 ℃ and 6% inoculation, which was comparable to the degradation of lignin in tobacco stems after three months of artificial aging.The content of total sugar and glucose increased 10.84% and 4.86% respectively, which eliminated the unpleasant odor of protein burning to certain extent and improved the wood gas in tobacco stem burning.
-
-
[1]
黄志强,包秀萍,高锐.烟梗及其制品提质处理的研究进展[J].河南农业科学,2013,42(10):1.
-
[2]
周瑢,陶红,沈光林,等.碱氧与酶处理改善梗丝性质的研究[J].现代食品科技,2010,26(5):463.
-
[3]
闫克玉,闫洪洋,李兴波,等.烤烟烟叶细胞壁物质的对比分析[J].烟草科技,2005(10):6.
-
[4]
张永良,周晓微,梁萌.烟梗综合利用研究进展[J].现代农业科技,2016(8):232.
-
[5]
王娜,李仙,王定伟.烟草木质素降解菌的筛选及在烟草中的应用[J].云南农业大学学报,2008(1):64.
-
[6]
TUOMELA M,VIKMAN M.,HATAKKA A,et al.Biodegradation of lignin in a compost environment:a review[J]. Bioresource Technology,2000,72(2):169.
-
[7]
杨虹琦,周冀衡,罗泽民,等.微生物和酶在烟叶发酵中的应用[J].湖南农业科学,2004(6):63.
-
[8]
黄静文,段焰青,者为,等.短小芽孢杆菌改善烟叶品质的研究[J].烟草科技,2010(8):61.
-
[9]
林凯.酶法对烟梗丝降解效果的研究[J].安徽农业科学,2011,39(11):6500.
-
[10]
周元清,周丽清,章新,等.用生物技术降解木质素提高烟梗使用价值初步研究[J].玉溪师范学院学报,2006,6(22):61.
-
[11]
朱大恒,韩锦峰,周御风.利用产香微生物发酵生产烟用香料技术及其应用[J].烟草科技,1997(1):30.
-
[12]
GUERRA A,MENDONCA R,FERRAZ A,et al.Structural characterization of lignin during pinus taeda wood treatment with Ceriporiopsis subvermispora[J].Applied and Environmental Microbiology,2004,70(7):4073.
-
[13]
徐海娟,梁文芷.白腐菌降解木素酶系及其作用机理[J].环境污染治理技术与设备,2000,1(3):51.
-
[14]
陈兴,申晓峰,巩效伟,等.利用微生物制剂提高梗丝品质的研究[J].中国烟草学报,2013,19(3):83.
-
[15]
迟建国.白腐菌对烟叶木质素含量的影响[J].贵州农业科学,2013,41(7):138.
-
[16]
刘志昌,毛耀,姚元军,等.烟梗木质素的白腐菌Coriolus versicolor T42降解研究[J].纸和造纸,2012,31(11):46.
-
[17]
国家烟草专卖局.烟草及烟草制品水溶性糖的测定连续流动法:YC/T 159-2002[S].北京:中国标准出版社,2002.
-
[18]
国家烟草专卖局.烟草及烟草制品总氮的测定克达尔法:YC/T 33-1996[S].北京:中国标准出版社,1999.
-
[19]
国家烟草专卖局.烟草及烟草制品总植物碱的测定光度法:YC/T 34-1996[S].北京:中国标准出版社,1999.
-
[20]
SLUITER A,HAMES B,RUIZ R,et al.Determination of structural carbohydrates and lignin in biomass[J].Laboratory Analytical Procedure,2008,1617:1.
-
[21]
王瑞新,韩富根.烟草化学品质分析法[M].郑州:河南科学技术出版社,1990.
-
[22]
李炎强,张峻松,贾会,等.烤烟水溶液值分析方法研究[J].烟草科技,2003(11):24.
-
[1]
计量
- PDF下载量: 15
- 文章访问数: 1284
- 引证文献数: 0