马铃薯蛋白微凝胶对皮克林乳液乳化特性的影响
Effect of potato protein microgel on emulsifying properties of Pickering emulsion
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摘要: 将不同质量分数(2%,4%,6%,8%)的马铃薯蛋白制成微凝胶(PPM2,PPM4,PPM6,PPM8),考察微凝胶的粒径、Zeta-电位、荧光特性、乳化活性、乳化稳定性和微观结构,研究马铃薯蛋白微凝胶对皮克林乳液乳化特性的影响.结果表明:随着马铃薯蛋白质量分数的增加,微凝胶颗粒的粒径显著升高(116.31~181.99 nm);PPM2,PPM4,PPM6的Zeta-电位均在30 mV左右,高于对照组;PPM2,PPM4,PPM6的荧光强度高于对照组,而PPM8的荧光强度低于对照组;与对照组相比,微凝胶可显著提高皮克林乳液的乳化活性和乳化稳定性;对照组和所有马铃薯蛋白微凝胶的微观结构表面光滑,对照组的马铃薯蛋白微观结构呈圆球状,而蛋白微凝胶微观结构呈片状聚集,且随着马铃薯蛋白质量分数的增大,聚集单元也越大.Abstract: Potato protein microgel (PPM2,PPM4,PPM6,PPM8) were prepared at different mass fractions (2%, 4%, 6%, 8%).The particle size, Zeta-potential, fluorescence characteristic, emulsifying activity, emulsion stability and microstructure of of microgel were investigated. The effect of potato protein microgel on the emulsification characteristics of Pickering emulsion was studied. The results showed that the particle size of the microgel particles increased significantly (116.31~181.99 nm) with the increase of protein fraction during microgel preparation. The Zeta-potentials of PPM2, PPM4 and PPM6 were about 30 mV and higher than that of the control group. The fluorescence intensity of PPM2, PPM4 and PPM6 was higher than that of the control group, while the fluorescence intensity of PPM8 was lower than that of the control group. Compared with the control group, the microgel significantly improved the emulsion activity and emulsion stability of the Pickering emulsion.The microstructure results showed that the surface of the control group and all potato protein microgel were smooth, the control group was spherical, the potato protein microgel was aggregated in a sheet form, and the aggregation unit was larger with the increase of the potato protein mass fraction.
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Key words:
- potato protein /
- microgel /
- Pickering emulsion /
- emulsifying property
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