亚硝化-厌氧氨氧化工艺的启动及微生物种群演替规律研究

张肖静; 张涵; 周月; 陈召; 张楠; 位登辉

doi:10.3969/j.issn.2096-1553.2019.06.008

CN 41-1437/TS ISSN 2096-1553

张肖静 , 张涵 , 周月 , 陈召 , 张楠 , 位登辉

张肖静, 张涵, 周月, 等. 亚硝化-厌氧氨氧化工艺的启动及微生物种群演替规律研究[J]. 轻工学报, 2019, 34(6): 56-63. doi: 10.3969/j.issn.2096-1553.2019.06.008

引用本文: 张肖静, 张涵, 周月, 等. 亚硝化-厌氧氨氧化工艺的启动及微生物种群演替规律研究[J]. 轻工学报, 2019, 34(6): 56-63. doi: 10.3969/j.issn.2096-1553.2019.06.008

ZHANG Xiaojing, ZHANG Han, ZHOU Yue, et al. Start-up and microbial community shift of partial nitrification-anammox process[J]. Journal of Light Industry, 2019, 34(6): 56-63. doi: 10.3969/j.issn.2096-1553.2019.06.008

Citation: ZHANG Xiaojing, ZHANG Han, ZHOU Yue, et al. Start-up and microbial community shift of partial nitrification-anammox process[J]. Journal of Light Industry, 2019, 34(6): 56-63. doi: 10.3969/j.issn.2096-1553.2019.06.008

亚硝化-厌氧氨氧化工艺的启动及微生物种群演替规律研究

郑州轻工业大学河南省化工分离过程强化工程技术研究中心/环境污染治理与生态修复河南省协同创新中心, 河南郑州 450001

作者简介: 张肖静(1986-),女,河南省开封市人,郑州轻工业大学副教授,博士,主要研究方向为环境污染治理新技术.;

基金项目: 国家自然科学基金项目（41701569）；郑州轻工业大学2018年度校级青年骨干教师项目
中图分类号: X703.1

Start-up and microbial community shift of partial nitrification-anammox process

He'nan Engineering Research Center of Chemical Engineering Separation Process Intensification/He'nan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, Zhengzhou 450001, China
Received Date: 2019-06-26

CLC number: X703.1

摘要: 在SBR反应器中接种普通活性污泥，在温度为22~26℃，pH值为7.8~8.0，DO值低于0.1 mg/L，进水氨氮质量浓度为400 mg/L的条件下，历时21 d，亚氮积累率稳定在95%以上，表明亚硝化工艺启动成功；在厌氧氨氧化工艺启动阶段，将反应时间调整为曝气7 h，厌氧搅拌16 h，历时35 d，总氮去除率达到70%以上，表明亚硝化-厌氧氨氧化工艺启动成功.在反应器的不同阶段，变形菌门的相对丰度从83.64%降低至10.09%，浮霉菌门的相对丰度则从1.63%增加到8.1%.
- 亚硝化 /
- 厌氧氨氧化 /
- 自养脱氮 /
- 快速启动 /
- 微生物种群
Abstract: The ordinary activated sludge was inoculated in the SBR reactor, and the temperature was 22~26℃, the pH was 7.8 to 8.0, the DO value was less than 0.1 mg/L, and the ammonia nitrogen concentration in the feed water was 400 mg/L.After 21 d,the nitrogen accumulation rate was stable above 95% and the partial nitrosation was started successfully; then the conditions of ammonia nitrogen concentration, reaction time, water exchange ratio and other conditions were changed to stabilize the operation to 75 d; during the anaerobic ammonia oxidation start-up phase, the reaction time was adjusted to 7 hours of aeration and 16 hours of anaerobic, the total nitrogen removal rate reached more than 70% after 35 days,and the partial nitrosation-anaerobic ammonia oxidation process started successfully. In different stages of the reactor, the relative abundance of proteobacteria was reduced from 83.64% to 10.09%; the relative abundance of floccus was increased from 1.63% to 8.1%.
- partial nitrification /
- anammox /
- autotrophic nitrogen removal /
- rapid start-up /
- microbial community
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沈阳化工大学材料科学与工程学院沈阳 110142

张肖静, 张涵, 周月, 等. 亚硝化-厌氧氨氧化工艺的启动及微生物种群演替规律研究[J]. 轻工学报, 2019, 34(6): 56-63. doi: 10.3969/j.issn.2096-1553.2019.06.008

亚硝化-厌氧氨氧化工艺的启动及微生物种群演替规律研究

作者简介:张肖静(1986-),女,河南省开封市人,郑州轻工业大学副教授,博士,主要研究方向为环境污染治理新技术.

郑州轻工业大学河南省化工分离过程强化工程技术研究中心/环境污染治理与生态修复河南省协同创新中心, 河南郑州 450001

收稿日期: 2019-06-26

基金项目: 国家自然科学基金项目（41701569）；郑州轻工业大学2018年度校级青年骨干教师项目

关键词:

摘要: 在SBR反应器中接种普通活性污泥，在温度为22~26℃，pH值为7.8~8.0，DO值低于0.1 mg/L，进水氨氮质量浓度为400 mg/L的条件下，历时21 d，亚氮积累率稳定在95%以上，表明亚硝化工艺启动成功；在厌氧氨氧化工艺启动阶段，将反应时间调整为曝气7 h，厌氧搅拌16 h，历时35 d，总氮去除率达到70%以上，表明亚硝化-厌氧氨氧化工艺启动成功.在反应器的不同阶段，变形菌门的相对丰度从83.64%降低至10.09%，浮霉菌门的相对丰度则从1.63%增加到8.1%.

English Abstract

Start-up and microbial community shift of partial nitrification-anammox process

He'nan Engineering Research Center of Chemical Engineering Separation Process Intensification/He'nan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Received Date: 2019-06-26

Abstract: The ordinary activated sludge was inoculated in the SBR reactor, and the temperature was 22~26℃, the pH was 7.8 to 8.0, the DO value was less than 0.1 mg/L, and the ammonia nitrogen concentration in the feed water was 400 mg/L.After 21 d,the nitrogen accumulation rate was stable above 95% and the partial nitrosation was started successfully; then the conditions of ammonia nitrogen concentration, reaction time, water exchange ratio and other conditions were changed to stabilize the operation to 75 d; during the anaerobic ammonia oxidation start-up phase, the reaction time was adjusted to 7 hours of aeration and 16 hours of anaerobic, the total nitrogen removal rate reached more than 70% after 35 days,and the partial nitrosation-anaerobic ammonia oxidation process started successfully. In different stages of the reactor, the relative abundance of proteobacteria was reduced from 83.64% to 10.09%; the relative abundance of floccus was increased from 1.63% to 8.1%.

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