蛋白酶水解中药渣制备生物活性肽工艺探讨
Process discussion on preparation of bioactive peptide in Chinese medicine residues by protease hydrolysis
-
摘要: 以中成药血必净药渣为材料,研究了通过蛋白酶水解从中制备生物活性肽的工艺.通过考察比较酸性蛋白酶、中性蛋白酶和碱性蛋白酶水解药渣的效果,确定了复合蛋白酶的适宜配比;通过比较不同水解时间的蛋白提取率、水解度和DPPH自由基清除率等指标,确定了复合蛋白酶适宜的水解时间.结果为,选用中性蛋白酶与碱性蛋白酶进行复配来水解制备生物活性肽,适宜配比为 3 GA6FA 1,4 h为复合蛋白酶适宜的水解时间.在该条件下蛋白质提取率达81%,DPPH 自由基清除率达39.8%.Abstract: Using the residues of Chinese meicine Xuebijing as materials, bioactive peptide was prepared by protease hydrolysis. The hydrolysis residues effect of acid protease, neutral protease and alkaline protease was investigated. On this basis, the appropriate proportion of compound protease hydrolysis residues was studied, and the suitable hydrolysis time of compound protease was confirmed by comparing the index of different hydrolysis time, such as protein extraction rate, hydrolysis degree and the antioxidant index DPPH free radical clearance rate. The results showed that the neutral protease compounded with alkaline protease was selected to prepare bioactive peptides, appropriate ratio was 3 :1; the suitable hydrolysis time of complex protease was 4 h, under the condition, the protein extraction rate was 81%, and the antioxidant index DPPH free radical clearance rate was 39.8%.
-
Key words:
- Chinese medicine residue /
- bioactive peptide /
- protease /
- hydrolysis
-
-
[1]
SONG X Y,LI Y D,SHI Y P.Quality control of traditional Chinese medicines:a review[J].Chinese journal of natural medicines,2013,11(6):596.
-
[2]
CHUNG V C H,HILLIER S.Referral to and attitude towards traditional Chinese medicine amongst western medical doctors in postcolonial Hong Kong[J].Social science & medicine,2011,72(2):247.
-
[3]
ZHANG H,TAN C,WANG H Z.Study on the history of traditional Chinese medicine to treat diabetes[J].European journal of integrative medicine,2010,2(1):41.
-
[4]
LIU H X,WANG S R,LEI Y.Characteristics and dvantages of traditional Chinese medicine in the treatment of acute myocardial infarction[J].Journal of traditional Chinese medicine,2011,31(4):269.
-
[5]
朱国婷.酶解补中益气丸药渣制备水不溶性膳食纤维工艺的研究[D].杭州:浙江大学, 2014:11-12.
-
[6]
庄毅,洪净.药用真菌双向性固体发酵工程与中成药药渣再开发[J].中国中药杂志,2006,31(22):1918.
-
[7]
陈缤,贾天柱.中药渣的综合利用[J].中成药,2005,27(10):1203.
-
[8]
高发奎.甘草废渣提取物中的氨基酸含量[J].甘肃环境研究与监测,1995,8(4):6.
-
[9]
贾伍员,秦坤,刘林.板蓝根药渣成分的测定及其利用研究[J].秦山医学院学报,2010,38(9):520.
-
[10]
祖庸, 刘建利, 雷闫盈.地衣类植物药渣的饲用价值研究[J].饲料研究,1991(4):15.
-
[11]
赵新淮,冯志彪.蛋白质水解物水解度的测定[J].食品科学, 1994, 179 (11):65.
-
[12]
郭兴凤.蛋白质水解度的测定[J].中国油脂, 2000, 25(6):176.
-
[13]
YEN W J,CHANG L W,LEE C P,et al.Inhibition of lipid peroxidation and nonlipid oxidative damage by carnosine[J].JAOCS,2002,79(4):329.
-
[14]
林诗云, 王炯,冯桂权,等.6 种天然药提取物清除自由基和抗氧化活性研究[J].云南民族大学学报(自然科学版), 2010, 19(3):204.
-
[1]
计量
- PDF下载量: 67
- 文章访问数: 1167
- 引证文献数: 0