甾醇分子差异对脂质体结构稳定性的影响
Effect of sterol molecular difference on the structural stability of liposomes
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摘要: 通过添加胆固醇、 β-谷甾醇和豆甾醇,采用乙醇注入法结合动态高压微流射技术制备甾醇脂质体,研究不同甾醇对脂质体膜结构稳定性的影响。结果表明:β-谷甾醇和豆甾醇的加入,使甾醇脂质体粒径从(48.35±0.41)nm分别增至(106.27±0.90)nm和(107.27±0.59)nm,Zeta电位保持不变,均在-30 mV左右;电子显微镜观察发现,添加甾醇的脂质体形成了稳定的磷脂双分子层结构;甾醇可以使脂质体的盐稳定性和pH稳定性增强,但对温度稳定性无显著影响;β-谷甾醇和豆甾醇的加入使脂质体膜的流动性、疏水性和微极性都显著减小,这表明脂质体磷脂双分子膜的结构变得更加致密。Abstract: Liposomes were prepared by ethanol injection combined with dynamic high-pressure microfluidic technology. The effects of different sterol molecular, including cholesterol, β-sitosterol and stigmasterol, on the membrane structural stability of liposome were compared. The results showed that the addition of β-sitosterol and stigmasterol increased the particle size of liposomes from (48.35±0.41) nm to (106.27±0.90) nm and (107.27±0.59) nm, respectively, and the Zeta potential remained unchanged at about -30 mV. Electron microscopy showed that the lipid containing sterol formed stable bilayer structure of phospholipid. The salt stability and pH stability had been enhanced, but temperature stability had no significant change. The addition of β-sitosterol and stigmasterol reduced the micropolarity, hydrophobicity and fluidity of liposome membrane, resulting in a tighter phospholipid bimolecular membrane structure in liposomes.
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Key words:
- cholesterol /
- β-sitosterol /
- stigmasterol /
- liposomes /
- structural stability
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