明胶-壳聚糖-肉桂精油天然涂膜液的制备及其抑菌效果研究
Study on the preparation of gelatin-chitosan-cinnamon essential oil natural film coating and its antibacterial effect
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摘要: 采用自组装法制备明胶-壳聚糖-肉桂精油天然涂膜液，以天然涂膜液的粒径及其对肉桂精油的包埋率为指标，在单因素试验的基础上，利用正交试验确定制备天然涂膜液的最佳工艺，并采用抑菌圈法研究该天然涂膜液的抑菌效果.结果表明，制备天然涂膜液的最佳工艺为明胶质量浓度6 mg/mL，壳聚糖质量浓度3 mg/mL，肉桂精油质量浓度3 mg/mL，乳化剂质量浓度3 mg/mL，并调节溶液pH值至4.5，转速800 r/min，搅拌时间30 min；在此工艺条件下，天然涂膜液的粒径为（236.54±18.32） nm，对肉桂精油的包埋率为（46.78±2.61）%；天然涂膜液处理组的抑菌效果均优于壳聚糖组和明胶-壳聚糖组，说明肉桂精油的添加可提高明胶-壳聚糖涂膜液的抑菌效果.Abstract: The gelatin-chitosan-cinnamon essential oil natural coating solution was prepared by the self-assembly method, and the particle size of the natural coating solution and its encapsulation rate of cinnamon essential oil were used as indicators. On the basis of single factor test, orthogonal test was used to determine the optimal process for preparing natural coating solution. The antibacterial effect of the natural coating solution by the antibacterial circle method was studied. The results showed that the optimal process for the preparation of gelatin-chitosan-cinnamon essential oil natural coating solution was gelatin mass concentration of 6 mg/mL, chitosan mass concentration of 3 mg/mL, cinnamon essential oil mass concentration of 3 mg/mL, emulsifier mass concentration of 3 mg/mL, adjusting the pH of the solution to 4. 5, rotating speed of 800 r/min, stirring time of 30 min. The particle size of natural coating solution was (236. 54±18. 32) nm, and the encapsulation rate of cinnamon essential oil was (46. 78±2. 61)%. And the antibacterial effect of the natural coating solution treatment group was better than that of the chitosan and gelatin-chitosan groups. The addition of cinnamon essential oil could improve the inhibition effect of gelatin-chitosan coating solution.
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Key words:
- gelatin /
- chitosan /
- cinnamon essential oil /
- natural film coating /
- antibacterial effect
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