普洱茶醇提不同萃取物的体外抑菌活性研究

吉杰丽; 薛桥丽; 谷大海; 朱仁俊; 胡永金

doi:10.3969/j.issn.2095-476X.2015.3/4.004

CN 41-1437/TS ISSN 2096-1553

普洱茶醇提不同萃取物的体外抑菌活性研究

吉杰丽 , 薛桥丽 , 谷大海 , 朱仁俊 , 胡永金

吉杰丽, 薛桥丽, 谷大海, 等. 普洱茶醇提不同萃取物的体外抑菌活性研究[J]. 轻工学报, 2015, 30(3-4): 15-19. doi: 10.3969/j.issn.2095-476X.2015.3/4.004

引用本文: 吉杰丽, 薛桥丽, 谷大海, 等. 普洱茶醇提不同萃取物的体外抑菌活性研究[J]. 轻工学报, 2015, 30(3-4): 15-19. doi: 10.3969/j.issn.2095-476X.2015.3/4.004

JI Jie-li, XUE Qiao-li, GU Da-hai, et al. Antibacterial activity of different extracts of Pu'er tea alcohol extraction in vitro[J]. Journal of Light Industry, 2015, 30(3-4): 15-19. doi: 10.3969/j.issn.2095-476X.2015.3/4.004

Citation: JI Jie-li, XUE Qiao-li, GU Da-hai, et al. Antibacterial activity of different extracts of Pu'er tea alcohol extraction in vitro[J]. Journal of Light Industry, 2015, 30(3-4): 15-19. doi: 10.3969/j.issn.2095-476X.2015.3/4.004

普洱茶醇提不同萃取物的体外抑菌活性研究

云南农业大学食品科学技术学院, 云南昆明 650201;
云南农业大学图书馆, 云南昆明 650201;
云南农业大学人事处, 云南昆明 650201

通讯作者: 胡永金

基金项目: 云南省自然科学基金项目(2011FZ091)
国家自然科学基金项目(31060270)
中图分类号: TS202.3

Antibacterial activity of different extracts of Pu'er tea alcohol extraction in vitro

College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
Library, Yunnan Agricultural University, Kunming 650201, China;
Department of Personnel, Yunnan Agricultural University, Kunming 650201, China

Corresponding author: HU Yong-jin,

Received Date: 2015-02-08
Available Online: 2015-09-15
CLC number: TS202.3

摘要: 采用滤纸片扩散法研究了普洱茶95%醇提萃取物石油醚、氯仿、乙酸乙酯、正丁醇和萃取后剩余物对金黄色葡萄球菌(S.aureus)和大肠杆菌(E.coli)的体外抑菌效果及普洱茶醇提乙酸乙酯萃取物抑菌活性的稳定性.结果表明:上述4种普洱茶醇提萃取物对S.aureus和E.coli均有一定的抑制作用,其中,乙酸乙酯萃取物的抑菌效果最好,对两种受试菌抑菌圈直径分别为(31.88±0.24) mm和(22.81±0.31) mm,最小抑菌浓度MIC分别为0.47 mg/mL和0.35 mg/mL.乙酸乙酯萃取物稳定性实验结果表明:普洱茶醇提乙酸乙酯萃取物在不同温度和紫外光照条件下均具有良好的抑菌稳定性,但酸碱性对其抑菌活性影响明显,抑菌效果随pH值的增大而逐渐变差.
- 普洱茶 /
- 醇提萃取物 /
- 抑菌活性
Abstract: Using Pu'er tea as raw material,filter paper diffusion method was adopted to study the antibacterial activity of petroleum ether,chloroform,ethyl acetate,n-butyl alcohol and residues after extraction from Pu'er tea 95% alcohol extraction in vitro on S.aureus and E.coli.The antibacterial activity stability of ethyl acetate extracts was also studied.The results indicated that the above four Pu'er tea extracts had certain degree of inhibitory effect against S.aureus and E.coli.Among them,ethyl acetate extracts on S.aureus and E.coli inhibitory effect was the best,and bacteriostatic ring diameters of two kinds of subjects were (31.88±0.24) mm and (22.81±0.31) mm, MIC were 0.47 mg/mL and 0.35 mg/mL respectively.The ethyl acetate extracts stability experimental results showed that ethyl acetate extracts was very stable antibacterial activity to temperature and UV irradiation,but the acid and alkaline influenced its antimicrobial activity obviously,antibacterial activity became poor gradually with pH increasing.
- Pu'er tea /
- alcohol extraction /
- antibacterial activity
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  池哲翔 , 廖敏 , 史尚 , 李声毅 , 廖芸 , 丁冬 . 国外烟草活性成分提取及纤维材料利用现状与展望. 轻工学报, 2024, 0(0): -.
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吉杰丽, 薛桥丽, 谷大海, 等. 普洱茶醇提不同萃取物的体外抑菌活性研究[J]. 轻工学报, 2015, 30(3-4): 15-19. doi: 10.3969/j.issn.2095-476X.2015.3/4.004

引用本文: 吉杰丽, 薛桥丽, 谷大海, 等. 普洱茶醇提不同萃取物的体外抑菌活性研究[J]. 轻工学报, 2015, 30(3-4): 15-19. doi: 10.3969/j.issn.2095-476X.2015.3/4.004

普洱茶醇提不同萃取物的体外抑菌活性研究

通讯作者: 胡永金

云南农业大学食品科学技术学院, 云南昆明 650201;
云南农业大学图书馆, 云南昆明 650201;
云南农业大学人事处, 云南昆明 650201

收稿日期: 2015-02-08
网络出版日期: 2015-09-15

基金项目: 云南省自然科学基金项目(2011FZ091)国家自然科学基金项目(31060270)

关键词:

摘要: 采用滤纸片扩散法研究了普洱茶95%醇提萃取物石油醚、氯仿、乙酸乙酯、正丁醇和萃取后剩余物对金黄色葡萄球菌(S.aureus)和大肠杆菌(E.coli)的体外抑菌效果及普洱茶醇提乙酸乙酯萃取物抑菌活性的稳定性.结果表明:上述4种普洱茶醇提萃取物对S.aureus和E.coli均有一定的抑制作用,其中,乙酸乙酯萃取物的抑菌效果最好,对两种受试菌抑菌圈直径分别为(31.88±0.24) mm和(22.81±0.31) mm,最小抑菌浓度MIC分别为0.47 mg/mL和0.35 mg/mL.乙酸乙酯萃取物稳定性实验结果表明:普洱茶醇提乙酸乙酯萃取物在不同温度和紫外光照条件下均具有良好的抑菌稳定性,但酸碱性对其抑菌活性影响明显,抑菌效果随pH值的增大而逐渐变差.

English Abstract

Antibacterial activity of different extracts of Pu'er tea alcohol extraction in vitro

Corresponding author: HU Yong-jin,

College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
Library, Yunnan Agricultural University, Kunming 650201, China;
Department of Personnel, Yunnan Agricultural University, Kunming 650201, China

Received Date: 2015-02-08
Available Online: 2015-09-15

Abstract: Using Pu'er tea as raw material,filter paper diffusion method was adopted to study the antibacterial activity of petroleum ether,chloroform,ethyl acetate,n-butyl alcohol and residues after extraction from Pu'er tea 95% alcohol extraction in vitro on S.aureus and E.coli.The antibacterial activity stability of ethyl acetate extracts was also studied.The results indicated that the above four Pu'er tea extracts had certain degree of inhibitory effect against S.aureus and E.coli.Among them,ethyl acetate extracts on S.aureus and E.coli inhibitory effect was the best,and bacteriostatic ring diameters of two kinds of subjects were (31.88±0.24) mm and (22.81±0.31) mm, MIC were 0.47 mg/mL and 0.35 mg/mL respectively.The ethyl acetate extracts stability experimental results showed that ethyl acetate extracts was very stable antibacterial activity to temperature and UV irradiation,but the acid and alkaline influenced its antimicrobial activity obviously,antibacterial activity became poor gradually with pH increasing.