杜仲叶提取物绿色合成纳米铂颗粒及其美白作用
Green synthesis of platinum nanoparticles by Eucommia ulmoides Oliver leaf extract and its whitening effect
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摘要: 以杜仲叶提取物为还原剂和稳定剂,采用一步法绿色合成纳米铂颗粒(PtNPs),通过单因素试验分析反应条件对PtNPs颜色和浓度的影响,并研究PtNPs对酪氨酸酶活性的影响。结果表明:在一定范围内改变反应条件可调控合成PtNPs的颜色和浓度,适宜反应条件为反应时间60 min、反应温度90 ℃、氯铂酸浓度3.5 mmol/L、杜仲叶提取物质量浓度10.0 mg/mL,在该条件下制备的PtNPs粒径平均尺寸为(2.27±0.65) nm,具有面心立方结构,分布较均匀;PtNPs对体外蘑菇酪氨酸酶活性有较强的抑制作用,IC50为(6.98±0.76) μg/mL,显著降低了A375细胞内酪氨酸酶活性和UVB诱导的黑色素含量,可达到美白皮肤的效果。Abstract: Platinum nanoparticles (PtNPs) were synthesized by one-step green method using Eucommia ulmoides Oliver leaf extract as reducer and stabilizer. The effects of reaction conditions on the color and concentration of PtNPs were analyzed by single factor test, and the effect of PtNPs on tyrosinase activity was studied. The results showed that the color and yield of PtNPs could be controlled by changing the reaction conditions in a certain range. The suitable reaction conditions of each factor were reaction time of 60 min, reaction temperature of 90 ℃, chloroplatinic acid concentration of 3.5 mmol/L, Eucommia ulmoides Oliver leaf extract concentration of 10.0 mg/mL. Under these conditions, the average size of prepared PtNPs was (2.27±0.65)nm, with face-centered cubic structure and dispersed distribution. PtNPs had a strong inhibitory effect on tyrosinase activity in vitro, IC50 value was (6.98±0.76) μg/mL, which significantly reduced tyrosinase activity and melanin content induced by UVB in A375 cells, and could achieve the effect of whitening skin.
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