基于高通量测序技术的中温大曲中微生物群落多样性解析

胡晓龙; 王康丽; 牛广杰; 乔亚娟; 张玉; 何培新

doi:10.3969/j.issn.2096-1553.2019.04.004

CN 41-1437/TS ISSN 2096-1553

胡晓龙 , 王康丽 , 牛广杰 , 乔亚娟 , 张玉 , 何培新

胡晓龙, 王康丽, 牛广杰, 等. 基于高通量测序技术的中温大曲中微生物群落多样性解析[J]. 轻工学报, 2019, 34(4): 21-29. doi: 10.3969/j.issn.2096-1553.2019.04.004

引用本文: 胡晓龙, 王康丽, 牛广杰, 等. 基于高通量测序技术的中温大曲中微生物群落多样性解析[J]. 轻工学报, 2019, 34(4): 21-29. doi: 10.3969/j.issn.2096-1553.2019.04.004

HU Xiaolong, WANG Kangli, NIU Guangjie, et al. Analysis of microbial community diversity in medium temperature Daqu based on high-throughput sequencing technology[J]. Journal of Light Industry, 2019, 34(4): 21-29. doi: 10.3969/j.issn.2096-1553.2019.04.004

Citation: HU Xiaolong, WANG Kangli, NIU Guangjie, et al. Analysis of microbial community diversity in medium temperature Daqu based on high-throughput sequencing technology[J]. Journal of Light Industry, 2019, 34(4): 21-29. doi: 10.3969/j.issn.2096-1553.2019.04.004

基于高通量测序技术的中温大曲中微生物群落多样性解析

1. 郑州轻工业大学食品与生物工程学院/食品生产与安全河南省协同创新中心, 河南郑州 450001;
2. 河南省寿酒集团有限公司, 河南新乡 453000

作者简介: 胡晓龙(1984-),男,河南省开封市人,郑州轻工业大学讲师,博士,主要研究方向为白酒酿造.;

基金项目: 国家自然科学基金项目（31801535）；河南省重大科技专项项目（181100211400）；中国轻工业浓型白酒固态发酵重点实验室开放基金项目（2018JJ017）；郑州轻工业学院博士科研启动基金项目（2016BSJJ018）；河南省自然科学基金项目（182300410070）
中图分类号: TS261.1

Analysis of microbial community diversity in medium temperature Daqu based on high-throughput sequencing technology

1. School of Food and Bioengineering/Collaborative Innovation Center of Production and Safety, He'nan Province, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
2. He'nan Shoujiu Group Co., Ltd., Xinxiang 453000, China
Received Date: 2019-04-10

CLC number: TS261.1

摘要: 采用高通量测序技术对河南省某酒企中温大曲曲皮和曲心两个部位的真菌群落和细菌群落的多样性及其组成进行了研究.结果表明：1）中温大曲曲皮样品中真菌群落多样性和丰度均高于曲心样品；子囊菌门是大曲曲皮和曲心样品中的唯一优势菌门；大曲样品中共检测到15种真菌，其中扣囊复膜孢酵母、雪黄散囊菌和毕赤酵母sp1为曲皮和曲心样品中的优势真菌，扣囊复膜孢酵母在曲皮和曲心样品中的含量基本一致，而雪黄散囊菌和毕赤酵母sp1在曲皮和曲心样品中的含量差异较大.2）中温大曲曲心样品中细菌群落多样性高于曲皮样品，但其丰度低于曲皮样品；在大曲样品中共检测到28个属，其中肠杆菌属、乳杆菌属、乳球菌属、布氏杆菌属、肠球菌属和欧文氏菌属为大曲样品中的优势细菌属，除布氏杆菌属外，其他优势细菌属在曲皮和曲心样品中的含量差异较大.
- 白酒 /
- 中温大曲 /
- 微生物群落 /
- 高通量测序技术
Abstract: The diversity and structure of fungal and bacterial communities in Qupi and Quxin of medium temperature Daqu from one wine corporation located in He'nan were analyzed based on the high-throughput sequencing technology.The results showed that:1) The diversity and abundance of fungal community in Qupi were higher than those in Quxin samples,and Ascomycetes was the only dominant fungi detected both in Qupi and Quxin.A total of 15 species of fungi was detected in Daqu,of which Saccharomycopsis fibuligera,Eurotium niveoglaucum,Pichia sp1 were the dominant fungi in Qupi and Quxin samples.Among them,the content of Saccharomvcopsis fibuligera in Qupi was similar to that in Quxin,however,the contents of Eurotium niveoglaucum and Pichia sp1 in Qupi and Quxin samples were quite different.2) The diversity of bacterial community in Quxin of medium temperature Daqu was higher than that of Qupi,but its abundance was lower than that of Qupi.A total of 28 genera was detected in the Qupi and Quxin samples,of which Enterobacter,Lactobacillus,Lactococcus,Brucella,Enterococcus and Erwinia were dominant bacteria of Daqu samples.Expect for Brucella,the content of other dominant bacteria had a large difference in Qupi and Quxin.
- Chinese liquor /
- medium temperature Daqu /
- microbial community /
- high-throughput sequencing technology
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沈阳化工大学材料科学与工程学院沈阳 110142

胡晓龙, 王康丽, 牛广杰, 等. 基于高通量测序技术的中温大曲中微生物群落多样性解析[J]. 轻工学报, 2019, 34(4): 21-29. doi: 10.3969/j.issn.2096-1553.2019.04.004

基于高通量测序技术的中温大曲中微生物群落多样性解析

作者简介:胡晓龙(1984-),男,河南省开封市人,郑州轻工业大学讲师,博士,主要研究方向为白酒酿造.

1. 郑州轻工业大学食品与生物工程学院/食品生产与安全河南省协同创新中心, 河南郑州 450001;
2. 河南省寿酒集团有限公司, 河南新乡 453000

收稿日期: 2019-04-10

基金项目: 国家自然科学基金项目（31801535）；河南省重大科技专项项目（181100211400）；中国轻工业浓型白酒固态发酵重点实验室开放基金项目（2018JJ017）；郑州轻工业学院博士科研启动基金项目（2016BSJJ018）；河南省自然科学基金项目（182300410070）

关键词:

摘要: 采用高通量测序技术对河南省某酒企中温大曲曲皮和曲心两个部位的真菌群落和细菌群落的多样性及其组成进行了研究.结果表明：1）中温大曲曲皮样品中真菌群落多样性和丰度均高于曲心样品；子囊菌门是大曲曲皮和曲心样品中的唯一优势菌门；大曲样品中共检测到15种真菌，其中扣囊复膜孢酵母、雪黄散囊菌和毕赤酵母sp1为曲皮和曲心样品中的优势真菌，扣囊复膜孢酵母在曲皮和曲心样品中的含量基本一致，而雪黄散囊菌和毕赤酵母sp1在曲皮和曲心样品中的含量差异较大.2）中温大曲曲心样品中细菌群落多样性高于曲皮样品，但其丰度低于曲皮样品；在大曲样品中共检测到28个属，其中肠杆菌属、乳杆菌属、乳球菌属、布氏杆菌属、肠球菌属和欧文氏菌属为大曲样品中的优势细菌属，除布氏杆菌属外，其他优势细菌属在曲皮和曲心样品中的含量差异较大.

English Abstract

Analysis of microbial community diversity in medium temperature Daqu based on high-throughput sequencing technology

1. School of Food and Bioengineering/Collaborative Innovation Center of Production and Safety, He'nan Province, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
2. He'nan Shoujiu Group Co., Ltd., Xinxiang 453000, China

Received Date: 2019-04-10

Abstract: The diversity and structure of fungal and bacterial communities in Qupi and Quxin of medium temperature Daqu from one wine corporation located in He'nan were analyzed based on the high-throughput sequencing technology.The results showed that:1) The diversity and abundance of fungal community in Qupi were higher than those in Quxin samples,and Ascomycetes was the only dominant fungi detected both in Qupi and Quxin.A total of 15 species of fungi was detected in Daqu,of which Saccharomycopsis fibuligera,Eurotium niveoglaucum,Pichia sp1 were the dominant fungi in Qupi and Quxin samples.Among them,the content of Saccharomvcopsis fibuligera in Qupi was similar to that in Quxin,however,the contents of Eurotium niveoglaucum and Pichia sp1 in Qupi and Quxin samples were quite different.2) The diversity of bacterial community in Quxin of medium temperature Daqu was higher than that of Qupi,but its abundance was lower than that of Qupi.A total of 28 genera was detected in the Qupi and Quxin samples,of which Enterobacter,Lactobacillus,Lactococcus,Brucella,Enterococcus and Erwinia were dominant bacteria of Daqu samples.Expect for Brucella,the content of other dominant bacteria had a large difference in Qupi and Quxin.