斑马鱼胚胎行为学在化妆品原料舒缓功效评价中的应用
Application of zebrafish embryos behavior in soothing efficacy evaluation of cosmetic ingredients
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摘要: 基于斑马鱼胚胎行为学建立斑马鱼刺激模型,分别采用该模型和传统斑马鱼炎症模型对甘草酸二钾和黄芩素的舒缓功效进行验证对比;利用斑马鱼刺激模型筛选评价α-红没药醇、丹皮酚、积雪草苷、原花青素和4-叔丁基环己醇5种化妆品原料的舒缓效果。结果表明:与4-丁基间苯二酚相比,十二烷基硫酸钠(SDS)是稳定性更好的造模剂;以SDS浓度500 μmol/L、对斑马鱼胚胎作用时间15 min构建的斑马鱼刺激模型评价甘草酸二钾和黄芩素的舒缓功效,结果与斑马鱼炎症模型基本一致;在高浓度下,α-红没药醇、丹皮酚、积雪草苷、原花青素在斑马鱼刺激模型中表现出较好的舒缓效果。Abstract: In this research, a zebrafish stimulation model was established based on zebrafish embryonic behavior. The soothing effects of dipotassium glycyrrhizinate and baicalein were verified and compared with the traditional zebrafish inflammation model. Then, the soothing efficacy of five cosmetic raw materials including α-red myrrh alcohol, paeonol, asiaticoside, proantho cyanidins and 4-tert-butylcyclohexanol was selectively evaluated by this model. The results showed that compared with 4-butyl-resorcinol, sodium dodecyl sulfate was a more stable modeling agent. The zebrafish stimulation model constructed by SDS concentration of 500 μmol/L and 15 min on zebrafish embryos was used to evaluate the soothing effects of dipotassium glycyrrhizinate and baicalein, and the results were basically consistent with the zebrafish inflammation model. At high concentrations, α-red myrrh alcohol, paeonol, asiaticoside and proantho cyanidins showed significantly soothing efficacy in the zebrafish stimulation model.
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Key words:
- zebrafish embryo /
- behavior /
- cosmetic ingredient /
- efficacy evaluation
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[1]
国家药品监督管理局.国家药监局关于发布《化妆品分类规则和分类目录》的公告(2021年第49号)[EB/OL].(2021-04-08
)[2021-12-18].https://www.nmpa.gov.cn/xxgk/ggtg/qtggtg/20210409160151122.html. -
[2]
王欢,盘瑶.化妆品功效评价(Ⅴ):舒缓功效宣称的科学支持[J].日用化学工业,2018,48(5):247-254.
-
[3]
张晓娥,李潇,卢永波."LPS——巨噬细胞"典型炎症模型评价化妆品抗炎舒敏功效的研究[C]//中国毒理学会.2017(第三届)毒性测试替代方法与转化毒理学(国际)学术研讨会会议论文集.2017:193-194.
-
[4]
孔雪,赵华,唐颖.皮肤模型在化妆品功效评价中的应用研究进展[J].日用化学工业,2017,47(4):228-231
,236. -
[5]
夏高辉,孔秋婵,陈庆生,等.19种舒敏特色植物提取物的筛选、组方及其在化妆品中的应用[J].广东轻工职业技术学院学报,2021,20(3):1-8.
-
[6]
ROEDING J,MAURIN V.4-叔丁基环己醇:敏感肌肤的"舒缓调节器"[J].陆婕,梅鹤祥,译.中国化妆品(行业),2010(5):79-81.
-
[7]
王奇,延在昊,何泉泉.斑马鱼模型在化妆品研究中的应用[J].日用化学品科学,2014,37(12):29-33.
-
[8]
MCGRATH P,LI C Q.Zebrafish:A predictive model for assessing drug-induced toxicity[J].Drug Discovery Today,2008,13(9/10):394-401.
-
[9]
南京新环检测科技有限公司.一种评价化妆品对皮肤炎症舒缓或修复功效的方法:CN202010874695.1[P].2020-12-11.
-
[10]
SAINT A L,DRAPEAU P.Time course of the development of motor behaviors in the zebrafish embryo[J].Journal of Neurobiology,1998,37(4):622-632.
-
[11]
郭樱子,栾亚楠,周玉玲,等.低剂量氯氟氰菊酯对斑马鱼胚胎运动行为的影响[J].温州医科大学学报,2016,46(7):476-481.
-
[12]
杨景峰,刘建宇,近藤麻子,等.多氯联苯引起斑马鱼胚胎自旋运动增加及基理[J].医学动物防制,2010,26(2):106-109.
-
[13]
SELDERSLAGHS I W,HOOYBERGHS J,DECOEN W,et al.Locomotor activity in zebrafish embryos:A new method to assess developmental neurotoxicity[J].Neurotoxicology and Teratology,2010,32(4):460-471.
-
[14]
KIMMEL C B,BALLARD W W,KIMMEL S R,et al.Stages of embryonic development of the zebrafish[J].Developmental Dynamics,1995,203(3):253-310.
-
[15]
赵丹,苏宁,张佳婵,等.十二烷基硫酸钠对角质细胞基因表达的影响及作用通路研究[J].日用化学工业,2016,46(6):334-338.
-
[16]
LAPEERE H,KEYSER E D.Allergic contact dermatitis caused by 4-n-butylresorcinol present in a night cream for skin hyperpigmentation[J].Contact Dermatitis,2020,83(2):134-135.
-
[17]
郑晓霞,谢晓芳.甘草酸二钾的抗炎作用研究及其在牙膏中的应用[J].口腔护理用品工业,2017,27(5):18-21.
-
[18]
区梓聪,何秋星,李馨恩,等.黄芩素在化妆品中多功效研究及安全性评价[J].天然产物研究与开发,2016,28(12):2006-2010
,1958. -
[19]
施米丽,金家瑞,郭美仙,等.甘草酸二钾护理液皮肤修复作用的研究[J].井冈山大学学报(自然科学版),2018,39(5):81-86.
-
[20]
许哲,李慧,黄勇,等.高安全性富勒烯化妆品原料及其美白、抗炎、修复功效[J].精细化工,2021,38(9):1881-1887.
-
[21]
郑岚,马耀宏,孟庆军,等.黑蒜多糖的斑马鱼体内抗氧化、抗炎症活性分析[J].中国食品学报,2020,20(8):65-75.
-
[22]
李馨恩.中药黄芩在化妆品中的多功效研究及其安全性评价[D].广州:广东药科大学,2016.
-
[23]
MAURYA A K,SINGH M,DUBEY V,et al.α-(-)-bisabolol reduces pro-inflammatory cytokine production and ameliorates skin inflammation[J].Current Pharmaceutical Biotechno-logy,2014,15(2):173-181.
-
[24]
刘宇超,白彦萍,王磊.丹皮酚药理作用及应用于皮肤病相关研究进展[J].新中医,2021,53(23):18-22.
-
[25]
温伟红,崔龙生,湛彩艳,等.一种积雪草脂质体在化妆品的研究应用[J].广东化工,2019,46(2):68-70.
-
[26]
张妍,吴秀香.原花青素研究进展[J].中药药理与临床,2011,27(6):112-116.
-
[27]
SULZBERGER M,WORTHMANN A C,HOLTZMANN U,et al.Effective treatment for sensitive skin:4-t-butylcyclohexanol and licochalcone A[J].Journal of the European Academy of Dermatology and Venereology,2016,30(1):9-17.
-
[28]
HALL A C,GRIFFITH T N,TSIKOLIA M,et al.Cyclohexanol analogues are positive modulators of GABA (A) receptor currents and act as general anaesthetics in vivo[J].European Journal of Pharmacology,2011,667(1):175-181.
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