JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

基于离子液体的菌藻类胡萝卜素提取工艺研究

张志平 ,  宋洋洋 ,  王秋领 ,  王清福 ,  龚贵平 ,  付瑜锋 ,  陈高

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张志平, 宋洋洋, 王秋领, 等. 基于离子液体的菌藻类胡萝卜素提取工艺研究[J]. 轻工学报, 2024, 39(2): 19-27. doi: 10.12187/2024.02.003
引用本文: 张志平, 宋洋洋, 王秋领, 等. 基于离子液体的菌藻类胡萝卜素提取工艺研究[J]. 轻工学报, 2024, 39(2): 19-27. doi: 10.12187/2024.02.003
shu
ZHANG Zhiping, SONG Yangyang, WANG Qiuling, et al. Research on extraction process of carotenoids from yeasts and algae based on ionic liquid[J]. Journal of Light Industry, 2024, 39(2): 19-27. doi: 10.12187/2024.02.003
Citation: ZHANG Zhiping, SONG Yangyang, WANG Qiuling, et al. Research on extraction process of carotenoids from yeasts and algae based on ionic liquid[J]. Journal of Light Industry, 2024, 39(2): 19-27. doi: 10.12187/2024.02.003
shu

基于离子液体的菌藻类胡萝卜素提取工艺研究

  • 1. 郑州轻工业大学 食品与生物工程学院, 河南 郑州 450001;

  • 2. 河南中烟工业有限责任公司 技术中心, 河南 郑州 450003;

  • 3. 农业农村部成都沼气科学研究所, 四川 成都 610042;

  • 4. 山东省农业科学研究院, 山东 济南 250100

    • 作者简介: 张志平(1981-),男,河南省安阳县人,郑州轻工业大学副教授,博士,主要研究方向为生物质资源化利用。E-mail:zzp@zzuli.edu.cn;

  • 基金项目: 河南省科技攻关项目(212102310077)
    中原科技创新领军人才项目(224200510017)

  • 中图分类号: TS202.3

Research on extraction process of carotenoids from yeasts and algae based on ionic liquid

  • 1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;

  • 2. Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450003, China;

  • 3. Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610042, China;

  • 4. Shandong Academy of Agricultural Science, Jinan 250100, China

  • Received Date: 2023-10-24
    Accepted Date: 2024-02-24

    CLC number: TS202.3

  • 摘要: 以含有粘红酵母和小球藻的菌藻泥为原料,通过单因素试验和响应面试验对菌藻类胡萝卜素提取工艺进行优化,并考查菌藻类胡萝卜素的体外抗氧化活性。结果表明:菌藻破壁的最优离子液体为1-丁基-3-甲基咪唑氯化物(BMIMCl),菌藻类胡萝卜素的最佳提取工艺为离子液体浓度1 mol/L、预处理温度53 ℃和预处理时间57 min,在该条件下,菌藻类胡萝卜素的提取量为1.311 mg/g;当质量浓度为30 μg/mL时,菌藻类胡萝卜素对DPPH自由基、·OH自由基和ABTS+自由基的清除率分别为82.3 %、37.6 %、和86.8 %,还原力为0.289,表现出较好的抗氧化活性。因此,经BMIMCl破壁处理后,从菌藻泥中提取的类胡萝卜素含量更高,抗氧化活性也较好。
    Abstract: Using Rhodotorula glutinis and Chlorella vularis sludge as raw material, the extraction process of carotenoids from yeast and algae was optimized through single factor and response experiments, and the in vitro antioxidant activity of carotenoids from yeast and algae was investigated. The results showed that the optimal ionic liquid for yeast and algal wall breaking was 1-butyl-3-methylimidazole chloride (BMIMCl), and the optimal extraction process for carotene from yeast and algal sludge was 1 mol/L ionic liquid concentration, pre-treatment temperature of 53 ℃, and pre-treatment time of 57 min. Under this condition, the extraction amount of carotene from yeast and algae was 1.311 mg/g. When the mass concentration was 30 μg/mL, the scavenging rates of DPPH free radical, ·OH free radical and ABTS+ free radical were 82.3 %, 37.6 % and 86.8 %, respectively, and the reducing power was 0.289, showing good antioxidant activity. The yeast and algal sludge treated with BMIMCl had a higher content of extracted carotenoids and better antioxidant activity.
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张志平, 宋洋洋, 王秋领, 等. 基于离子液体的菌藻类胡萝卜素提取工艺研究[J]. 轻工学报, 2024, 39(2): 19-27. doi: 10.12187/2024.02.003
引用本文: 张志平, 宋洋洋, 王秋领, 等. 基于离子液体的菌藻类胡萝卜素提取工艺研究[J]. 轻工学报, 2024, 39(2): 19-27. doi: 10.12187/2024.02.003
shu
ZHANG Zhiping, SONG Yangyang, WANG Qiuling, et al. Research on extraction process of carotenoids from yeasts and algae based on ionic liquid[J]. Journal of Light Industry, 2024, 39(2): 19-27. doi: 10.12187/2024.02.003
Citation: ZHANG Zhiping, SONG Yangyang, WANG Qiuling, et al. Research on extraction process of carotenoids from yeasts and algae based on ionic liquid[J]. Journal of Light Industry, 2024, 39(2): 19-27. doi: 10.12187/2024.02.003
shu

基于离子液体的菌藻类胡萝卜素提取工艺研究

    作者简介:张志平(1981-),男,河南省安阳县人,郑州轻工业大学副教授,博士,主要研究方向为生物质资源化利用。E-mail:zzp@zzuli.edu.cn
  • 1. 郑州轻工业大学 食品与生物工程学院, 河南 郑州 450001;
  • 2. 河南中烟工业有限责任公司 技术中心, 河南 郑州 450003;
  • 3. 农业农村部成都沼气科学研究所, 四川 成都 610042;
  • 4. 山东省农业科学研究院, 山东 济南 250100
  • 收稿日期: 2023-10-24
  • 录用日期: 2024-02-24
基金项目:  河南省科技攻关项目(212102310077)中原科技创新领军人才项目(224200510017)

摘要: 以含有粘红酵母和小球藻的菌藻泥为原料,通过单因素试验和响应面试验对菌藻类胡萝卜素提取工艺进行优化,并考查菌藻类胡萝卜素的体外抗氧化活性。结果表明:菌藻破壁的最优离子液体为1-丁基-3-甲基咪唑氯化物(BMIMCl),菌藻类胡萝卜素的最佳提取工艺为离子液体浓度1 mol/L、预处理温度53 ℃和预处理时间57 min,在该条件下,菌藻类胡萝卜素的提取量为1.311 mg/g;当质量浓度为30 μg/mL时,菌藻类胡萝卜素对DPPH自由基、·OH自由基和ABTS+自由基的清除率分别为82.3 %、37.6 %、和86.8 %,还原力为0.289,表现出较好的抗氧化活性。因此,经BMIMCl破壁处理后,从菌藻泥中提取的类胡萝卜素含量更高,抗氧化活性也较好。

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