带鱼蛋白酶解工艺条件的响应面优化
Response surface optimization of proteolysis technological conditions of hairtail
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摘要: 以带鱼为原材料，从6种蛋白酶中筛选出碱性蛋白酶作为带鱼蛋白酶解的最佳蛋白酶，进而利用单因素试验和响应面试验优化带鱼蛋白酶解的工艺条件.结果表明：带鱼蛋白酶解的最佳工艺条件为底物质量分数5.3%，蛋白酶质量分数2.7%，酶解pH值8.3，酶解温度63℃，酶解时间168 min，在该工艺条件下，带鱼蛋白水解度达到37.07%，与响应面优化试验回归模型的预测值基本一致.Abstract: Taking hairtail as raw material,alkaline protease was selected from 6 kinds of proteases as the best protease for hairtail proteolysis,and the technological conditions of hairtail proteolysis were optimized by single factor test and response surface test.The results showed that the optimum conditions for proteolysis of hairtail were as follows:the substrate mass fraction was 5.3%,the protease mass fraction was 2.7%,the pH value of hydrolysis was 8.3,the hydrolysis temperature was 63℃,and the hydrolysis time was 168 min.Under these conditions,the hydrolysis degree of hairtail protein reached 37.07%,which was basically consistent with the predicted value of regression model of response surface optimization test.
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Key words:
- hairtail /
- proteolysis /
- alkaline protease /
- response surface optimization
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[1]
刘云国,李八方,汪东风,等.海洋生物活性肽研究进展[J].中国海洋药物,2005,24(3):52.
-
[2]
HARNEDY P A,FITZGERALD R J.Bioactive peptides from marine processingwaste and shellfish:A review[J].Journal of Functional Foods,2012,4(1):6.
-
[3]
崔欣,效梅,安立龙,等.海洋生物活性肽的生理功能及其应用研究进展[J].广东农业科学,2013,40(9):103.
-
[4]
管华诗,韩玉谦,冯晓梅.海洋活性多肽的研究进展[J].中国海洋大学学报,2004,34(5):761.
-
[5]
GILDBERG A,ARNESEN J A,CARLEHOG M M.Utilisation of cod backbone by biochemical fractionation[J].Process Biochemistry,2002,38:475.
-
[6]
邱秀宝,袁影,戴宏,等.嗜碱性芽孢杆菌碱蛋白酶的研究Ⅱ:诱变株选育及产酶条件[J].微生物学报,1990,30(2):129.
-
[7]
GBOGOURI G A,LINDER M,FANNI J,et al.Influence of hydrolysis degree on the functional properties of salmon byproducts hydrolysates[J].Journal of Food Science,2004,69(8):615.
-
[8]
张群飞,徐大伦,杨文鸽.响应面法优化碱性蛋白酶酶解虾籽的工艺条件[J].核农学报,2014,28(5):876.
-
[9]
OVISSIPOUR M,ABEDIAN K A,MOTAMEDZADEGAN A,et al.Optimization of enzymatic hydrolysis of visceral waste proteins of Yellowfin tuna (Thunnus albacares)[J].Food and Bioprocess Technology,2012,5(2):696.
-
[10]
ADLER-NISSEN J.Enzymic hydrolysis of food proteins[M].London:Elsevier Applied Science Publishers,1986:116.
-
[11]
林慧敏.带鱼下脚料酶解小肽亚铁螯合物结构鉴定及其生物活性研究[D].福州:福建农林大学,2012.
-
[12]
毋瑾超,汪依凡,方长富.鲢鱼降血压肽的酶解条件[J].湛江海洋大学学报(自然科学),2006(1):67.
-
[13]
王雪芹,邢荣娥,刘松,等.鲐鱼蛋白酶解工艺优化及其抗氧化活性测定[J].现代食品科技,2013,29(5):1023.
-
[14]
朱少娟,施用晖,乐国伟.超声波对胰蛋白酶水解酪蛋白的影响[J].食品与生物技术学报,2005,24(2):116.
-
[15]
李学鹏,刘晏玮,高郡焕.美拉德反应改良四角蛤蜊酶解液的风味[J].食品科学,2018,39(16):82.
-
[16]
魏鉴腾,刘永峰.酶解法制备鱼籽多肽的工艺研究[J].食品工业,2015,36(1):71.
-
[17]
KRAJEWSKA B.Application of chitin and chitosan-based materials for enzyme immobilizations:A review[J].Enzyme and Microbial Technology,2004,35(2):126.
-
[18]
WETTASINGHE M,VASANTHAN T,TEMELLI F,et al.Volatiles from roasted by products of the poultry processing industry[J].Journal of Agriculture and Food Chemistry,2000,48(8):3485.
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