嗜热麦芽糖转葡萄糖基酶的制备及其应用综述
Review of research advances on preparation and applications of amylomaltases from thermus scotoductu
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摘要: 针对目前国内外关于嗜热麦芽糖转葡萄糖基酶的制备及应用进展进行了综述:主要采用基因克隆至常温宿主细胞中,通过宿主细胞的过量表达来完成制备,其应用主要体现在淀粉的优化改性、大环糊精的制备以及功能成分的糖基化修饰等方面.未来研究将集中于酶的多功能催化机制,同时拓展糖基化改性功能成分的种类及转糖基类型.
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关键词:
- 嗜热麦芽糖转葡萄糖基酶 /
- 淀粉优化改性 /
- 糖基化改性
Abstract: The production of amylomaltases and its promising applications were reviewed.The preparation was carried out through gene cloning to normal temperature host cell and the host cell excessive expression and the application reflected in modification of starches,production of large-ring cyclodextrins and transglycosylation of some functional ingredients,etc.It was further pointed out that the future research will focuse on multifunctional catalytic mechanism of the enzyme and increasing the varieties of glycosylation reactions as well as their functonal products. -
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[1]
Imamura H,Fushinobu S,Jeon B S,et al.Identification of the catalytic residue of Thermococcus litoralis 4-α-glucanotransferase through mechanism-based labeling[J].Biochemistry,2001,40(41):12400.
-
[2]
Takara T,Yanase M,Takata H,et al.Cyclic glucans produced by the intramolecular transglycosylation activity of potato D-enzyme on amylopectin[J].Biochem Biophy Res Comm,1998,247:493.
-
[3]
李淑彬,陆广欣,林如妹,等.嗜热菌-工业用酶的新来源[J].中国生物工程杂志,2003,23(7):67.
-
[4]
Yoshinobu T,Kazutoshi F,Takeshi T.Thermus aquaticus ATCC 33923 amylomaltase gene cloning and expression and enzyme characterization:production of cycloamylose[J].Applied and Envir Microbiology,1999,65(3):910.
-
[5]
Yoshihisa T,Shinsuke F,Masahiro T,et al.Cloning and expression of the 4-α-glucanotransferase gene from the hyperthermophilic archaeon Pyrococcus sp.KOD1 and characterization of the enzyme[J].J of Fermentation and Bioeng,1997,83(6):540.
-
[6]
Kang H K,Jang J H,Shim J H.Efficient constitutive expression of thermostable 4-α-glucanotransferase in Bacillus subtilis using dual promoters[J].World J Microbiol Biotechnol,2010,26:1915.
-
[7]
Imamura H,Jeon B S,Wakagi T.High level expression of Thermococcus litoralis 4-α-glucanotransferase in a soluble form in Escherichia coli with a novel expression system involving minor arginine tRNAs and GroELS[J].FEBS Letters,1999,457(3):393.
-
[8]
Kim K Y,Kim C H.Expression of Thermotoga maritima 4-α-glucanotransferase gene in E.coli and characterization of the recombinant enzyme[J].Agric Chem Biotechnol,2004,47(3):133.
-
[9]
Kazutoshi F,Hirotaka M,Yoshinobu T,et al.Use of random and saturation mutageneses to improve the properties of Thermus aquaticus amylomaltase for efficient production of cycloamyloses[J].Applied and Envir Microbiology,2005,71(10):5823.
-
[10]
Lee K Y,Kim Y R,Park K H,et al.Effects of α-glucanotransferase treatment on the hermo-reversibility and freeze-thaw stability of a rice starch gel[J].Carbohydrate Polymers,2006,63:347.
-
[11]
Lee K Y,Kim Y R,Park K H.Rheological and gelation properties of rice starch modified with 4-α-glucanotransferase[J].Int J of Biological Macromolecules,2008,42(3):298.
-
[12]
Cho K H,Auh J H,Ryu J H.Structural modification and characterization of rice starch treated by Thermus aquaticus 4-α-glucanotransferase[J].Food Hydrocolloids,2009,23:2403.
-
[13]
Mun S H,Kim Y L,Kang C G.Development of reduced-fat mayonnaise using 4-α GTase-modified rice starch and xanthan gum[J].Int J of Biological Macromolecules,2009,44(5):400.
-
[14]
Marc J E C,Isabelle C,Euverink G W,et al.A novel thermoreversible gelling product made by enzymatic modification of starch[J].Starch,2005,57:465.
-
[15]
Park J H,Park H K.Physicochemical properties of enzymatically modified maize starch using 4-α-glucanotransferase[J].Food Sci and Biotech,2007,16(6):902.
-
[16]
Oh E J,Choi S J,Lee S J.Modification of granular corn starch with 4-α-glucanotransferase from Thermotoga maritima:Effects on structural and physical properties[J].J of Food Sci,2008,73(3):C158.
-
[17]
Ueda H.Physicochemical properties and complex formation abilities of large-ring cyclodextrins[J].J of Inclusion Phenomena and Macrocyclic Chemistry,2002,44:53.
-
[18]
Kin L L.Large cyclodextrins[J].J of Inclusion Phenomena and Macrocyclic Chemistry,2002,43:1.
-
[19]
Lee C K,Le Q T,Kim Y H.Enzymatic synthesis and properties of highly branched rice starch amylose and amylopectin cluster[J].J Agric Food Chem,2008,56(1):126.
-
[20]
Kajiura H,Kakutani R,Akiyama T,et al.A novel enzymatic process for glycogen production[J].Biocatal Biotransform,2008,26:133.
-
[21]
Shim J H,Seo N S,Roh S A,et al.Improved bread-baking process using Saccharomyces cerevisiae displayed with engineered cyclodextrin glucanotransferase[J].J Agric Food Chem,2007,55:4735.
-
[22]
Seo N S,Roh S A,Auh J H,et al.Structural characterization of rice starch in rice cake modified by Thermus scotoductus 4-α-glucanotransferase (TSαGTase)[J].J of Food Sci,2007,72(6):C331.
-
[23]
Mun S,Kim Y L,Kang C G,et al.Development of reduced-fat mayonnaise using 4-α-GTase-modified rice starch and xanthan gum[J].Int J of Biological Macromolecules,2009,44:400.
-
[24]
方继前,郭亚平,谢练武.生物转化法定向结构改造难溶性药物[J].现代化工,2010,30(8):32.
-
[25]
Li D,Roh S A,Shim J H,et al.Glycosylation of genistin into soluble inclusion complex form of cyclic glucans by enzymatic modification[J].J Agric Food Chem,2005,53(16):6516.
-
[26]
Chung M J,Kang A Y,Lee K M,et al.Water-soluble genistin glycoside isoflavones up-regulate antioxidant metallothionein expression and scavenge free radicals[J].J Agric Food Chem,2006,54:3819.
-
[27]
Li D,Park S H,Shim J H,et al.In vitro enzymatic modification of puerarin to puerarin glycosides by maltogenic amylase[J].Carbohydrate Research,2004,339:2789.
-
[28]
Chung M J,Sung N J,Park C S,et al.Antioxidative and hypocholesterolemic activities of water-soluble puerarin glycosides in HepG2 cells and in C57 BL/6J mice[J].European J of Pharmacology,2008,578:159.
-
[29]
Abelyan V A,Balayan A M,Ghochikyan V T.Transglycosylation of stevioside by cyclodextrin glucanotransferases of various groups of microorganisms[J].Applied Biochemistry and Microbiology,2004,40(2):129.
-
[30]
Markosyan A A,Abelyan L A,Adamyan M O.Transglycosylation of L-ascorbic acid[J].Applied Biochemistry and Microbiology,2007,43(1):36.
-
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