JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

农田土壤中病原真菌DNA提取方法的研究

王楠 ,  王伟

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王楠, 王伟. 农田土壤中病原真菌DNA提取方法的研究[J]. 轻工学报, 2013, 28(2): 54-58,63. doi: 10.3969/j.issn.2095-476X.2013.02.013
引用本文: 王楠, 王伟. 农田土壤中病原真菌DNA提取方法的研究[J]. 轻工学报, 2013, 28(2): 54-58,63. doi: 10.3969/j.issn.2095-476X.2013.02.013
shu
WANG Nan and WANG Wei. Study on an extraction method of pathogenic fungal DNA from farmland soil[J]. Journal of Light Industry, 2013, 28(2): 54-58,63. doi: 10.3969/j.issn.2095-476X.2013.02.013
Citation: WANG Nan and WANG Wei. Study on an extraction method of pathogenic fungal DNA from farmland soil[J]. Journal of Light Industry, 2013, 28(2): 54-58,63. doi: 10.3969/j.issn.2095-476X.2013.02.013
shu

农田土壤中病原真菌DNA提取方法的研究

  • 郑州轻工业学院 食品与生物工程学院, 河南 郑州 450001;

  • 华东理工大学 生物反应器工程国家重点实验室, 上海 200237

    • 通讯作者: 王伟

  • 基金项目: 国家863计划项目(2012AA101401)
    国家公益性行业(农业)专项(201003029)

  • 中图分类号: S154.3

Study on an extraction method of pathogenic fungal DNA from farmland soil

  • College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;

  • State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

    • Corresponding author: WANG Wei,
  • Received Date: 2013-03-05
    Available Online: 2013-03-15

    CLC number: S154.3

  • 摘要: 通过比较不同的研磨、洗涤、裂解和沉淀方法,成功获得一种适于土壤真菌DNA提取的方法.结果表明,采用石英砂研磨、TENP和PBS联合洗涤、SDS高盐裂解、异丙醇沉淀等一系列步骤,所获取的土壤微生物DNA质量最佳.该试验方法可以直接从土壤中提取微生物基因组,尤其适用于土壤病原真菌的提取,所得DNA完全可以直接用于酶切和PCR扩增.该方法高效、价廉、操作简单,对改进土壤病原菌生态的研究方法具有重要价值.
    Abstract: By comparing grinding method, washing method, cleavage and precipitation method, a method was gotten suitable for farmland fungi DNA extraction. The results showed that the quality of soil microorganism DNA is of the best which is obtained by grinding with quartz sand, washing with TENP and PBS, lysing with SDS in high-salt buffer and precipitating with isopropanol and so on. The pathogenic fungal DNA can be directly extracted from soil by using the method, which is particularly suitable for the soil pathogenic fungal extraction. The DNA obtained can be directly used for restriction enzyme digestion and PCR amplification. The method is efficient, inexpensive, easy to operate, which is of important value for the soil pathogens ecological research.
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  • 通讯作者:  王伟,
  • 收稿日期:  2013-03-05
  • 刊出日期:  2013-03-15
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王楠, 王伟. 农田土壤中病原真菌DNA提取方法的研究[J]. 轻工学报, 2013, 28(2): 54-58,63. doi: 10.3969/j.issn.2095-476X.2013.02.013
引用本文: 王楠, 王伟. 农田土壤中病原真菌DNA提取方法的研究[J]. 轻工学报, 2013, 28(2): 54-58,63. doi: 10.3969/j.issn.2095-476X.2013.02.013
shu
WANG Nan and WANG Wei. Study on an extraction method of pathogenic fungal DNA from farmland soil[J]. Journal of Light Industry, 2013, 28(2): 54-58,63. doi: 10.3969/j.issn.2095-476X.2013.02.013
Citation: WANG Nan and WANG Wei. Study on an extraction method of pathogenic fungal DNA from farmland soil[J]. Journal of Light Industry, 2013, 28(2): 54-58,63. doi: 10.3969/j.issn.2095-476X.2013.02.013
shu

农田土壤中病原真菌DNA提取方法的研究

    通讯作者: 王伟
  • 郑州轻工业学院 食品与生物工程学院, 河南 郑州 450001;
  • 华东理工大学 生物反应器工程国家重点实验室, 上海 200237
  • 收稿日期: 2013-03-05
  • 网络出版日期: 2013-03-15
基金项目:  国家863计划项目(2012AA101401)国家公益性行业(农业)专项(201003029)

摘要: 通过比较不同的研磨、洗涤、裂解和沉淀方法,成功获得一种适于土壤真菌DNA提取的方法.结果表明,采用石英砂研磨、TENP和PBS联合洗涤、SDS高盐裂解、异丙醇沉淀等一系列步骤,所获取的土壤微生物DNA质量最佳.该试验方法可以直接从土壤中提取微生物基因组,尤其适用于土壤病原真菌的提取,所得DNA完全可以直接用于酶切和PCR扩增.该方法高效、价廉、操作简单,对改进土壤病原菌生态的研究方法具有重要价值.

English Abstract

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