脱脂米糠联产丁醇、植酸盐、米糠蛋白和米糠膳食纤维工艺的研究
Study on co-production of butanol,phytate,rice bran protein and rice bran dietary fiber with defatted rice bran
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摘要: 通过高温α-淀粉酶液化、液态发酵、酸浸、碱性蛋白酶酶解、喷雾干燥等工艺操作,以脱脂米糠(DRB)为原料联产丁醇、植酸盐、米糠蛋白和米糠膳食纤维,研究DRB综合利用过程的工艺条件和所制备产品的理化性质.结果表明:适宜DRB综合利用的工艺流程顺序为DRB高温液化发酵产丁醇、分离植酸盐、提取米糠蛋白和米糠膳食纤维.所得产物中,丁醇在发酵液中的质量浓度达14.03 g/L;植酸盐为白色粉末,提取率6.20%,P2O5含量达到39.77%,符合中国药典药用植酸盐标准;米糠蛋白提取率12.11%,氨基酸总含量达63.32%,必需氨基酸组成与鸡蛋蛋白相当,过敏性低,适合用作儿童食品的添加蛋白;米糠膳食纤维提取率25.00%,膳食纤维纯度72.00%,可作为功能食品添加剂添加到各类保健食品中.Abstract: Defatted rice bran was taken as material, by high temperature alpha amylase liquefaction, liquid fermentation, acid leaching, alkaline protease hydrolysis and spray drying process, the butanol, phytate, rice bran protein and rice bran dietary fiber were co-produced. The process conditions and physicochemical properties of the products were studied. The results showed that the extraction sequence suitable for defatted rice bran was DRB liquefaction at high temperature,butanal fermentation,phytate, extract rice bran protein and rice bran dietary fiber separation. The mass concentration of butanol in the fermentation broth was up to 14.03 g/L. The extraction rate of phytate was 6.20%, while the content of P2O5 was 39.77%, which fully met the standard of Chinese Pharmacopoeia. The extraction rate of rice bran protein was 12.11%, while the content of total amino acids was 63.32%. The composition of essential amino acids was comparable to that of egg protein, which had low allergy and was suitable for use in children's food. The extraction rate of rice bran dietary fiber was 25.00%, while the content of dietary fiber was 72.00%, which could be added to various health foods as functional food additives.
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Key words:
- defatted rice bran (DRB) /
- butanol /
- phytate /
- rice bran protein /
- rice bran dietary fiber
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