白腐菌液体发酵降解烟梗木质素的研究
Study on the degradation of lignin in tobacco stem by liquid fermentation with white rot fungi
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摘要: 以白腐菌P. chrysosporium为对象,采用连续传代驯化的方法提升菌体对烟碱的耐受度.通过比较人工陈化与液体发酵对烟梗中木质素的降解效率,并对其发酵条件进行优化.结果表明,驯化后的白腐菌P. chrysosporium可有效定植于烟梗上,在34℃,接种量6%的最适发酵条件下液体发酵24 h,烟梗中木质素含量为1.93%,降解了23.11%,与人工陈化3个月烟梗木质素降解情况相当,总糖和葡萄糖含量分别增加10.84%和4.86%,在一定程度上消除了蛋白质燃烧产生的不良气味,改善了烟梗燃烧时产生的木质气.Abstract: The white rot fungus (P.Chrysosporium) was subjected to continuous subcultivation and domestication to improve the nicotine tolerance of the fungus. The degradation efficiency of lignin in tobacco stem was compared between artificial aging and liquid fermentation to obtain the optimal fermentation conditions. The results showed that the domesticated white rot fungi was effectively planted on tobacco stems. The content of lignin in tobacco stems reached 1.93%, decreasing by 23.11% after 24 h of liquid fermentation under the optimum fermentation conditions of 34 ℃ and 6% inoculation, which was comparable to the degradation of lignin in tobacco stems after three months of artificial aging.The content of total sugar and glucose increased 10.84% and 4.86% respectively, which eliminated the unpleasant odor of protein burning to certain extent and improved the wood gas in tobacco stem burning.
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