款冬叶黄酮的抗氧化活性研究
Research on the antioxidant activity of coltsfoot leaf flavonoid
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摘要: 采用超声波辅助提取款冬叶黄酮,利用吸附树脂对其进行纯化,进而探讨款冬叶黄酮体外抗氧化活性(DPPH+·清除力和Fe3+还原力)及其对亚急性衰老模型小鼠体内抗氧化活性(T-AOC,SOD活性,GSH-PX活性和MDA含量)的影响.结果表明:款冬叶黄酮具有良好的抗氧化活性,可提高DPPH+·清除力和Fe3+还原力,并可显著提高亚急性衰老模型小鼠体内的T-AOC,SOD活性和GSH-PX活性,降低MDA含量.款冬叶黄酮良好的抗氧化活性可应用于抗衰老产品的研究,为我国中草药活性成分的提取与应用提供参考.Abstract: Using ultrasonic auxiliary to extract coltsfoot leaf flavonoid,and to purify it by adsorption resin,coltsfoot leaf flavonoid in vitro antioxidant activity (DPPH+·scavenging force and Fe3+ reducing force) and its influence on antioxidant activity of subacute aging model mice (T-AOC,SOD activity,GSH-PX activity and MDA content) were explored.The results showed that the coltsfoot leaf flavonoid had good antioxidant activity,and they could improve the Fe3+ reducing force and DPPH+·scavenging force,and obviously improve the T-AOC,SOD and GSH-PX activity of the subacute aging model mice and reduce MDA content.The good antioxidant activity of coltsfoot leaf flavonoid could be applied to promote anti-aging research and provide a reference for the extraction and application of active ingredient in Chinese herbal medicine.
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Key words:
- coltsfoot leaf /
- flavonoid /
- aging model mice /
- antioxidant activity
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