节杆菌Z3粗酶液降解白肋烟烟碱条件优化
Conditions optimization of Arthrobacter Z3 crude enzyme solution degradate nicotine in the Burley tobacco
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摘要: 以节杆菌Z3(Arthrobacter Z3)为产酶菌株,通过正交实验,对烟碱脱氢酶降解白肋烟烟碱条件进行了研究.实验结果表明,最佳发酵条件为:酶液添加量50%,发酵温度33℃,发酵时间9 h,烟丝含水率70%.此条件下烟碱降解率为49.32%,使白肋烟烟碱含量达到了烤烟水平.这表明,粗酶液能显著降低白肋烟中的烟碱.Abstract: Using the Arthrobacter Z3 as enzyme production strains,the conditions of nicotine dehydrogenase degradation Burley nicotine was studied by orthogonal experiment.The results showed that the best fermentation conditions were:50% of the added amount of the enzyme solution, fermentation temperature 33℃, fermentation time 9 h, tobacco shreds moisture content 70%.Under these conditions nicotine degradation rate was 49.32% and Burley nicotine content reached the same level of flue-cured tobacco.The crude enzyme fluid can significantly reduce nicotine in Burley tobacco.
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Key words:
- Burley tobacco /
- nicotine degradation /
- fermentation condition /
- Arthrobacter Z3
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[1]
Gutierrez R.Nicotine degradation by bacteria Enterobacter cloacae as nicotine Degrader (Spain)[J].Anales de Instituto Nacional Investigaciones Agrarias Serie Agricola,1983,22:85.
-
[2]
Tashiro A,Yuji K,Yonemura C,et al.Pure isolation of nicotine-degradin microbes[J].Kyushu Kyoritsu Daigaku Kenkyu Hokoku Kogakubu,1996,20:147.
-
[3]
Mesnard F, Girard S, fliniaux O,et al.Chiral specificity of the degradation of nicotine by nicotiana plumbaginifolia cell suspension cultres[J].Plant Science,2001,161:1011.
-
[4]
王革,王颖琦,李松,等.微生物发酵烟叶降解尼古丁、蛋白质[J].烟草科学研究,2001(2):66.
-
[5]
马林,曾晓鹰,张峻松,等.烟碱脱氢酶基因的原核表达及酶学特性研究[J].中国烟草学报,2007,13(4):60.
-
[6]
马林,张峻松,张文龙,等.产烟碱脱氢酶菌株节杆菌Z3的发酵条件研究[J].烟草科技,2007,51(10):60.
-
[7]
闫克玉. 烟草化学[M].郑州:郑州大学出版社,2002:114.
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