扁枝槲寄生提取物体外抗氧化效应研究

张煜; 张晓娜; 薛桥丽; 肖蓉; 林奇; 胡永金

doi:10.3969/j.issn.2096-1553.2016.3.006

CN 41-1437/TS ISSN 2096-1553

扁枝槲寄生提取物体外抗氧化效应研究

张煜 , 张晓娜 , 薛桥丽 , 肖蓉 , 林奇 , 胡永金

张煜, 张晓娜, 薛桥丽, 等. 扁枝槲寄生提取物体外抗氧化效应研究[J]. 轻工学报, 2016, 31(3): 39-48. doi: 10.3969/j.issn.2096-1553.2016.3.006

引用本文: 张煜, 张晓娜, 薛桥丽, 等. 扁枝槲寄生提取物体外抗氧化效应研究[J]. 轻工学报, 2016, 31(3): 39-48. doi: 10.3969/j.issn.2096-1553.2016.3.006

ZHANG Yu, ZHANG Xiao-na, XUE Qiao-li, et al. Determination of antioxidant activity of Visum ardiculatum in vitro[J]. Journal of Light Industry, 2016, 31(3): 39-48. doi: 10.3969/j.issn.2096-1553.2016.3.006

Citation: ZHANG Yu, ZHANG Xiao-na, XUE Qiao-li, et al. Determination of antioxidant activity of Visum ardiculatum in vitro[J]. Journal of Light Industry, 2016, 31(3): 39-48. doi: 10.3969/j.issn.2096-1553.2016.3.006

扁枝槲寄生提取物体外抗氧化效应研究

张煜 ¹ ,
张晓娜 ² ,
薛桥丽 ³ ,
肖蓉 ¹ ,
林奇 ¹ ,
胡永金 ^{1

,}

云南农业大学食品科学技术学院, 昆明云南 650201;
河南科技学院新科学院, 河南新乡 453003;
云南农业大学图书馆, 昆明云南 650201

通讯作者: 胡永金

基金项目: 国家自然科学基金项目(31260404)
中图分类号: TS201;R285

Determination of antioxidant activity of Visum ardiculatum in vitro

Faculty of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
Xinke College, He'nan Institute of Science and Technology, Xinxiang 453003, China;
Library, Yunnan Agricultural University, Kunming 650201, China

Corresponding author: HU Yong-jin,

Received Date: 2015-12-17
Available Online: 2016-05-15
CLC number: TS201;R285

摘要: 采用水浴和超声两种提取方式,分别用水和无水乙醇提取扁枝槲寄生(俗称"螃蟹脚"),再用氯仿、乙酸乙酯和正丁醇萃取两种方式的水提取物,得到不同的萃取组分.分别测定各萃取组分的总游离氨基酸、茶多酚、茶多糖和总黄酮的含量,及其对DPPH自由基、ABTS自由基、超氧阴离子、羟基自由基的清除能力和FRAP值.结果表明,"螃蟹脚"具有良好的体外抗氧化活性,各提取-萃取组分对ABTS自由基的清除率最高均能够达到98%以上,对DPPH自由基的清除率最高均可达到88%以上.由模糊评价法得到的抗氧化综合值能够很好地反映各组分的总抗氧化能力,水浴水提取物综合值最高,为4.66,水浴水提取物的氯仿萃取层综合值最低,为0.21.其与茶多酚(P<0.05)和黄酮(P<0.01)的含量显著相关."螃蟹脚"中茶多糖的含量高达5468.08 mg/100 g.
- 扁枝槲寄生 /
- 抗氧化 /
- 相关性 /
- 模糊评价
Abstract: Visum ardiculatum was extracted by water bath and ultrasonic, and its four fractions of chloroform, ethyl acetate, n-butanol, and water were prepared. The free-amino acid, total polyphenol, tea polysaccharides and flavonoids contents Visum ardiculatum were determined. So were the scavenging effects of different parts of Visum ardiculatum on DPPH radical, ABTS radical, superoxide anion and hydroxyl radical and FRAP value. The results showed that the antioxidant activity of Visum ardiculatum in vitro was potent. The scavenging effects of all the fractions on ABTS radical reached 98% and that of DPPH radical reached 88%. The synthetical value calculated by fuzzy assessment was respective of the 5 antioxidant assays.The highest was 4.66 of water extracts by water bath, the lowest was 0.21 of chloroform fraction. It was largely due to the total polyphenol (P<0.05) and flavonoids contents (P<0.05).The content of tea polysaccharides reached 5 468.08 mg/100 g.
- Visum ardiculatum /
- antioxidant /
- correlation /
- fuzzy assessment
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张煜, 张晓娜, 薛桥丽, 等. 扁枝槲寄生提取物体外抗氧化效应研究[J]. 轻工学报, 2016, 31(3): 39-48. doi: 10.3969/j.issn.2096-1553.2016.3.006

引用本文: 张煜, 张晓娜, 薛桥丽, 等. 扁枝槲寄生提取物体外抗氧化效应研究[J]. 轻工学报, 2016, 31(3): 39-48. doi: 10.3969/j.issn.2096-1553.2016.3.006

扁枝槲寄生提取物体外抗氧化效应研究

通讯作者: 胡永金

云南农业大学食品科学技术学院, 昆明云南 650201;
河南科技学院新科学院, 河南新乡 453003;
云南农业大学图书馆, 昆明云南 650201

收稿日期: 2015-12-17
网络出版日期: 2016-05-15

基金项目: 国家自然科学基金项目(31260404)

关键词:

摘要: 采用水浴和超声两种提取方式,分别用水和无水乙醇提取扁枝槲寄生(俗称"螃蟹脚"),再用氯仿、乙酸乙酯和正丁醇萃取两种方式的水提取物,得到不同的萃取组分.分别测定各萃取组分的总游离氨基酸、茶多酚、茶多糖和总黄酮的含量,及其对DPPH自由基、ABTS自由基、超氧阴离子、羟基自由基的清除能力和FRAP值.结果表明,"螃蟹脚"具有良好的体外抗氧化活性,各提取-萃取组分对ABTS自由基的清除率最高均能够达到98%以上,对DPPH自由基的清除率最高均可达到88%以上.由模糊评价法得到的抗氧化综合值能够很好地反映各组分的总抗氧化能力,水浴水提取物综合值最高,为4.66,水浴水提取物的氯仿萃取层综合值最低,为0.21.其与茶多酚(P<0.05)和黄酮(P<0.01)的含量显著相关."螃蟹脚"中茶多糖的含量高达5468.08 mg/100 g.

English Abstract

Determination of antioxidant activity of Visum ardiculatum in vitro

Corresponding author: HU Yong-jin,

Faculty of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
Xinke College, He'nan Institute of Science and Technology, Xinxiang 453003, China;
Library, Yunnan Agricultural University, Kunming 650201, China

Received Date: 2015-12-17
Available Online: 2016-05-15

Abstract: Visum ardiculatum was extracted by water bath and ultrasonic, and its four fractions of chloroform, ethyl acetate, n-butanol, and water were prepared. The free-amino acid, total polyphenol, tea polysaccharides and flavonoids contents Visum ardiculatum were determined. So were the scavenging effects of different parts of Visum ardiculatum on DPPH radical, ABTS radical, superoxide anion and hydroxyl radical and FRAP value. The results showed that the antioxidant activity of Visum ardiculatum in vitro was potent. The scavenging effects of all the fractions on ABTS radical reached 98% and that of DPPH radical reached 88%. The synthetical value calculated by fuzzy assessment was respective of the 5 antioxidant assays.The highest was 4.66 of water extracts by water bath, the lowest was 0.21 of chloroform fraction. It was largely due to the total polyphenol (P<0.05) and flavonoids contents (P<0.05).The content of tea polysaccharides reached 5 468.08 mg/100 g.