烟叶中西柏三烯-4,6-二醇降解产香菌的分离与鉴定
Isolation and identification of a cembratriene-4,6-diol degradation and aroma producing strain from tobacco leaf
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摘要: 采取稀释涂布平板法和平板划线法,从调制陈化后的烟叶中分离得到一株高效降解西柏三烯-4,6-二醇的菌株YI1-3.以西柏三烯-4,6-二醇为唯一碳源,该菌株降解产物主要为金合欢醛(8.57%).根据形态特征和16S rDNA系统进化树分析,初步鉴定菌株YI1-3为新鞘氨醇杆菌(Novosphingobium panipatense).该菌株在含有西柏三烯-4,6-二醇发酵培养基中培养66 h后,底物降解率达到75.58%.
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关键词:
- 西柏三烯-4,6-二醇 /
- 分离鉴定 /
- 降解率 /
- 金合欢醛 /
- 新鞘氨醇杆菌
Abstract: A high efficient cembratriene-4,6-diol degradation strain YI1-3 was isolated from tobacco leaf with spread plate and streak plate methods. The cembratriene-4,6-diol was the only carbon source, and the major degradation products were farnesal (8.57%). This strain YI1-3 was identified preliminarily as Novosphingobium panipatense through the analysis of classical morphology and 16S rDNA sequence of phylogenetic tree. The strain was cultured in the fementation medium containing cembratriene-4,6-diol, the degradation rate of substrates reached 74.58% after 66 h. -
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