红景天提取液生化与细胞水平的美白功效研究
Study on whitening efficacy of rhodiola crenulata extract on the biochemistry and cell level
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摘要: 采用超声提取的方法制备红景天提取液，所得生药的质量浓度为0.05 g/mL.通过清除1，1-二苯基-2-三硝基苯肼（DPPH）自由基实验、抑制酪氨酸酶实验、小鼠B16黑色素瘤细胞实验，评估红景天提取液生化与细胞水平的美白功效.结果显示，在1.00 wt%的受试浓度下，红景天提取液对DPPH自由基的清除率为92.2%，对酪氨酸酶的抑制率为79.1%，黑色素细胞相对细胞活率高于75%，对小鼠黑色素细胞合成黑色素的抑制率为39.0%.说明所制备的红景天提取液具有良好的清除DPPH自由基活性和抑制酪氨酸酶活性的作用，可有效降低小鼠黑色素细胞分泌黑色素的能力，在生化与细胞水平具有良好的美白功效，在美白化妆品中具有良好的应用潜力.Abstract: The rhodiola crenulata extract was prepared by the method of ultrasonic extraction, and the quality concentration of the extract was 0.05 g/mL.The experiments of clearance rate of DPPH free radical and inhibition rate of tyrosinase were conducted to evaluate the whitening efficacy of biochemistry level of the rhodiola crenulata extract, and the test of mouse B16 melanoma cell was applied to evaluate the whitening efficacy of cell level.The results showed that under the concentration of 1.0 wt%, the clearance rate of DPPH was 92.2%, the inhibition rate of tyrosinase was 79.1%,melanoma cell comparative survival rate was up to 75%, and the inhibition rate of melanin synthesis to mouse B16 melanoma cell was 39.0%.It declared that the prepared rhodiola crenulata extract had good function of clearing DPPH free radical and inhibiting tyrosinase.Therefore, the rhodiola crenulata extract has excellent whitening efficacy on the biochemistry and cell level, which had a good potential to apply into the whitening cosmetics.
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Key words:
- rhodiola crenulata extract /
- antioxidant /
- whitening efficacy /
- cosmetics
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