发酵多肽Asp-Asp-Asp-Tyr和Asp-Tyr-Asp-Asp的稳定性研究

梁楚容; 王琴; 马路凯; 肖更生; 刘袆帆

doi:10.12187/2023.02.006

发酵多肽Asp-Asp-Asp-Tyr和Asp-Tyr-Asp-Asp的稳定性研究

1. 仲恺农业工程学院轻工食品学院, 广东广州 510225;
2. 广东省岭南特色食品科学与技术重点实验室, 广东广州 510225;
3. 西藏自治区农牧业科学院农产品开发与食品科学研究所, 西藏拉萨 850000

作者简介: 梁楚容(1997-),女,广东省肇庆市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:1027529290@qq.com;

基金项目: 国家自然科学基金项目(32001622)；广东省自然科学基金项目(2019A1515011283)；广州市基础研究计划基础与应用研究基础项目(202002030383)；江门市基础与应用基础研究重点项目(2021030103330007214)
中图分类号: TS225.1

Study on the stability of fermented polypeptides Asp-Asp-Asp-Tyr and Asp-Tyr-Asp-Asp

1. College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
2. Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou 510225, China;
3. Institute of Agricultural Product Development and Food Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China
Received Date: 2022-06-14

CLC number: TS225.1

摘要: 以DPPH和ABTS自由基清除率为考查指标,探究不同储存时间、pH值、金属离子、储存温度及人工模拟消化系统对兜唇石斛发酵多肽Asp-Asp-Asp-Tyr(DDDY)和Asp-Tyr-Asp-Asp(DYDD)抗氧化活性的影响,并进一步研究2种发酵多肽的稳定性。结果表明:随着储存时间的延长,DDDY和DYDD的DPPH和ABTS清除率均明显下降,DPPH清除率在储存16 h后分别下降了约73.43%和73.22%;DDDY和DYDD在酸性或弱酸性环境下的稳定性较好,当pH值为2.0~12.0时,其ABTS清除率分别下降了约66.00%和70.81%,而对DPPH清除率的影响不大;金属离子会明显提高2种发酵多肽的ABTS清除率,其中受Na⁺、K⁺和Fe³⁺这3种金属离子影响较大;过高和过低的储存温度均会降低2种发酵多肽的DPPH和ABTS清除率,其中DYDD的ABTS清除率受储存温度影响较大;DDDY和DYDD的抗氧化活性在人工模拟消化系统中随着消化作用时间的延长均明显降低。2种发酵多肽的稳定性受环境影响较大,未来可通过包埋或基团连接等方式深入研究提高其稳定性的路径。
- 兜唇石斛 /
- 发酵多肽 /
- 稳定性 /
- 抗氧化活性
Abstract: The effects of different storage time, pH value, metal ions, storage temperature and artificial simulation of digestive system on the antioxidant activities of fermented peptides Asp-Asp-Asp-Tyr (DDDY) and Asp-Tyr-Asp-Asp (DYDD) of Dendrobium aphyllum were investigated using DPPH and ABTS free radical scavenging rate as indexes. The stability of the two fermented peptides was further studied. The results showed that the DPPH and ABTS clearance rates of DDDY and DYDD decreased significantly with the increase of storage time, and the DPPH clearance rate decreased by about 73.43% and 73.22%, respectively, after 16 hours of storage. The stability of DDDY and DYDD was better in acidic or weakly acidic environment, and the ABTS clearance rate of DDDY and DYDD decreased by about 66.00% and 70.81% when the pH value was 2.0~12.0, respectively. Metal ions could significantly improve the ABTS clearance rate of the two fermented peptides, which was greatly affected by three metal ions: Na⁺, K⁺ and Fe³⁺. Too high and too low storage temperature decreased the DPPH and ABTS clearance rates of the two fermented peptides, and the ABTS clearance rate of DYDD was greatly affected by storage temperature. The antioxidant activity of DDDY and DYDD decreased significantly with the prolongation of digestion time in the artificial simulated digestive system. The stability of the two fermented peptides was greatly affected by the environments, and the path to improve their stability could be further studied through embedding or group linking in the future.
- Dendrobium aphyllum /
- fermented polypeptide /
- stability /
- antioxidant activity
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沈阳化工大学材料科学与工程学院沈阳 110142

发酵多肽Asp-Asp-Asp-Tyr和Asp-Tyr-Asp-Asp的稳定性研究

作者简介: 梁楚容(1997-),女,广东省肇庆市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:1027529290@qq.com;

Study on the stability of fermented polypeptides Asp-Asp-Asp-Tyr and Asp-Tyr-Asp-Asp

计量

通讯作者: 陈斌, bchen63@163.com

发酵多肽Asp-Asp-Asp-Tyr和Asp-Tyr-Asp-Asp的稳定性研究

作者简介:梁楚容(1997-),女,广东省肇庆市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:1027529290@qq.com

English Abstract

Study on the stability of fermented polypeptides Asp-Asp-Asp-Tyr and Asp-Tyr-Asp-Asp

目录

发酵多肽Asp-Asp-Asp-Tyr和Asp-Tyr-Asp-Asp的稳定性研究

作者简介: 梁楚容(1997-),女,广东省肇庆市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:1027529290@qq.com;

Study on the stability of fermented polypeptides Asp-Asp-Asp-Tyr and Asp-Tyr-Asp-Asp

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通讯作者: 陈斌, bchen63@163.com

发酵多肽Asp-Asp-Asp-Tyr和Asp-Tyr-Asp-Asp的稳定性研究

作者简介:梁楚容(1997-),女,广东省肇庆市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:1027529290@qq.com

English Abstract

Study on the stability of fermented polypeptides Asp-Asp-Asp-Tyr and Asp-Tyr-Asp-Asp

目录