JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

不同预处理方法对烟杆酶解产糖和结构特征的影响

宋丽丽 ,  张志平 ,  王光路 ,  杨旭 ,  张靖楠

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宋丽丽, 张志平, 王光路, 等. 不同预处理方法对烟杆酶解产糖和结构特征的影响[J]. 轻工学报, 2019, 34(3): 52-59. doi: 10.3969/j.issn.2096-1553.2019.03.006
引用本文: 宋丽丽, 张志平, 王光路, 等. 不同预处理方法对烟杆酶解产糖和结构特征的影响[J]. 轻工学报, 2019, 34(3): 52-59. doi: 10.3969/j.issn.2096-1553.2019.03.006
shu
SONG Lili, ZHANG Zhiping, WANG Guanglu, et al. Effect of different pretreatment methods to the enzymatic hydrolysis and structural characteristic of tobacco stalks[J]. Journal of Light Industry, 2019, 34(3): 52-59. doi: 10.3969/j.issn.2096-1553.2019.03.006
Citation: SONG Lili, ZHANG Zhiping, WANG Guanglu, et al. Effect of different pretreatment methods to the enzymatic hydrolysis and structural characteristic of tobacco stalks[J]. Journal of Light Industry, 2019, 34(3): 52-59. doi: 10.3969/j.issn.2096-1553.2019.03.006
shu

不同预处理方法对烟杆酶解产糖和结构特征的影响

  • 郑州轻工业大学 食品与生物工程学院/烟草工业生物技术重点实验室, 河南 郑州 450001

    • 作者简介: 宋丽丽(1987-),女,河南省信阳市人,郑州轻工业大学讲师,博士,主要研究方向为烟草废弃物综合利用.;

  • 基金项目: 国家自然科学基金项目（21706244）；河南省科技攻关项目（152102110104）；郑州轻工业学院博士基金项目（2013BSJJ005）

  • 中图分类号: TS49;TK6

Effect of different pretreatment methods to the enzymatic hydrolysis and structural characteristic of tobacco stalks

  • College of Food and Biological Engineering/Key Laboratory of Biotechnology in Tobacco Industry, Zhengzhou University of Light Industry, Zhengzhou 450001, China

  • Received Date: 2018-10-15

    CLC number: TS49;TK6

  • 摘要: 以烟草加工副产物烟杆为原料，将酸处理、碱处理和微波处理对烟杆酶解产糖的影响进行比较，并分析处理前后烟杆组分和微观结构的变化.结果表明，3种预处理方法中，碱处理对烟杆转化效率提升的作用较为显著，以质量分数为1%的NaOH溶液于75℃处理烟杆120 min，每g烟杆酶解液中葡萄糖质量为334.69 mg，较对照样品提高了118.37%.组分分析表明，碱处理对烟杆木质素的降解较为明显，降解率为46.45%.碱处理不仅破坏了烟杆木质素苯环骨架，还对苯环侧链基团进行了改性，造成木质素大分子结构解体，烟杆酶解抗性屏障作用降低，从而提高了烟杆的转化效率.碱处理并未改变烟杆纤维素结晶度，而微波处理和酸处理导致的结晶度升高反而会对提升烟杆酶解效率产生负面影响.
    Abstract: The effects of acid treatment, alkali treatment and microwave treatment on the enzymatic hydrolysis of tobacco stalks were studied by using tobacco processing by-product tobacco rods as raw materials, and the changes of composition and microstructure of tobacco stalks before and after treatment were analyzed. The results showed that the alkali treatment had a significant effect on the conversion efficiency of tobacco stalks in the three pretreatment methods. The tobacco stalks were treated with a mass fraction of 1% NaOH solution at 75℃ for 120 min, and the glucose content in the hydrolysate was 334.69 mg/g tobacco stalks increased by 118.37% compared to the control sample. The composition analysis showed that the degradation of tobacco lignin by alkali treatment was more obvious, and the degradation rate was 46.45%. Alkali treatment not only destroyed the tobacco lignin benzene ring skeleton, but also modified the benzene ring side chain group, resulting in the disintegration of the lignin macromolecular structure, and the tobacco stalks enzymatic resistance barrier function was reduced, thereby improving the conversion efficiency of tobacco stalks. Alkali treatment did not change the crystallinity of tobacco stalks cellulose, and the increase in crystallinity caused by microwave and acid treatment would adversely affected the efficiency of enzymatic hydrolysis of tobacco stalks.
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宋丽丽, 张志平, 王光路, 等. 不同预处理方法对烟杆酶解产糖和结构特征的影响[J]. 轻工学报, 2019, 34(3): 52-59. doi: 10.3969/j.issn.2096-1553.2019.03.006
引用本文: 宋丽丽, 张志平, 王光路, 等. 不同预处理方法对烟杆酶解产糖和结构特征的影响[J]. 轻工学报, 2019, 34(3): 52-59. doi: 10.3969/j.issn.2096-1553.2019.03.006
shu
SONG Lili, ZHANG Zhiping, WANG Guanglu, et al. Effect of different pretreatment methods to the enzymatic hydrolysis and structural characteristic of tobacco stalks[J]. Journal of Light Industry, 2019, 34(3): 52-59. doi: 10.3969/j.issn.2096-1553.2019.03.006
Citation: SONG Lili, ZHANG Zhiping, WANG Guanglu, et al. Effect of different pretreatment methods to the enzymatic hydrolysis and structural characteristic of tobacco stalks[J]. Journal of Light Industry, 2019, 34(3): 52-59. doi: 10.3969/j.issn.2096-1553.2019.03.006
shu

不同预处理方法对烟杆酶解产糖和结构特征的影响

    作者简介:宋丽丽(1987-),女,河南省信阳市人,郑州轻工业大学讲师,博士,主要研究方向为烟草废弃物综合利用.
  • 郑州轻工业大学 食品与生物工程学院/烟草工业生物技术重点实验室, 河南 郑州 450001
  • 收稿日期: 2018-10-15
基金项目:  国家自然科学基金项目（21706244）；河南省科技攻关项目（152102110104）；郑州轻工业学院博士基金项目（2013BSJJ005）

摘要: 以烟草加工副产物烟杆为原料，将酸处理、碱处理和微波处理对烟杆酶解产糖的影响进行比较，并分析处理前后烟杆组分和微观结构的变化.结果表明，3种预处理方法中，碱处理对烟杆转化效率提升的作用较为显著，以质量分数为1%的NaOH溶液于75℃处理烟杆120 min，每g烟杆酶解液中葡萄糖质量为334.69 mg，较对照样品提高了118.37%.组分分析表明，碱处理对烟杆木质素的降解较为明显，降解率为46.45%.碱处理不仅破坏了烟杆木质素苯环骨架，还对苯环侧链基团进行了改性，造成木质素大分子结构解体，烟杆酶解抗性屏障作用降低，从而提高了烟杆的转化效率.碱处理并未改变烟杆纤维素结晶度，而微波处理和酸处理导致的结晶度升高反而会对提升烟杆酶解效率产生负面影响.

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