JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

基于核酸适体传感器的黄曲霉毒素B1检测研究进展

姜利英 ,  刘帅 ,  张培 ,  任林娇 ,  闫艳霞 ,  郑晓婉 ,  陈青华 ,  姜素霞

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姜利英, 刘帅, 张培, 等. 基于核酸适体传感器的黄曲霉毒素B1检测研究进展[J]. 轻工学报, 2017, 32(2): 40-47. doi: 10.3969/j.issn.2096-1553.2017.2.007
引用本文: 姜利英, 刘帅, 张培, 等. 基于核酸适体传感器的黄曲霉毒素B1检测研究进展[J]. 轻工学报, 2017, 32(2): 40-47. doi: 10.3969/j.issn.2096-1553.2017.2.007
shu
JIANG Li-ying, LIU Shuai, ZHANG Pei, et al. Research progress of aflatoxin B1 detection based on nucleic acid aptasensor[J]. Journal of Light Industry, 2017, 32(2): 40-47. doi: 10.3969/j.issn.2096-1553.2017.2.007
Citation: JIANG Li-ying, LIU Shuai, ZHANG Pei, et al. Research progress of aflatoxin B1 detection based on nucleic acid aptasensor[J]. Journal of Light Industry, 2017, 32(2): 40-47. doi: 10.3969/j.issn.2096-1553.2017.2.007
shu

基于核酸适体传感器的黄曲霉毒素B1检测研究进展

  • 郑州轻工业学院 电气信息工程学院, 河南 郑州 450002

  • 基金项目: 国家自然科学基金项目（61002007）；河南省科技创新人才计划项目（124100510001）

  • 中图分类号: TP212.3;Q524

Research progress of aflatoxin B1 detection based on nucleic acid aptasensor

  • College of Electric Information Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China

  • Received Date: 2016-09-01
    Available Online: 2017-03-15

    CLC number: TP212.3;Q524

  • 摘要: 食物中存在毒性和致癌性较强的黄曲霉毒素B1（AFB1），目前常规的检测方法有薄层色谱法、高效液相色谱法、液相色谱-串联质谱法和免疫分析法，但都存在一定局限性.基于核酸适体传感器的AFB1检测研究取得了一定成果，基于电化学核酸适体传感器、光学核酸适体传感器和PCR核酸适体传感器的3种新型检测技术各有利弊，将血糖仪作为检测仪器、与核酸适体传感器相结合检测AFB1的思路有待实验验证.未来基于生物识别元件和磁性材料的磁亲和固相萃取法与核酸适体传感器相结合进行AFB1检测，有望获得突破.
    Abstract: There is a high toxicity and carcinogenicity of aflatoxin B1(AFB1) in food. Current detection of aflatoxin B1 is performed by a variety of methods including thin layer chromatography, high performance liquid chromatography, liquid chromatography-tandem mass spectrometry and immunoassay, but they all have some limitations. AFB1 detection research had obtained certain achievements based on nucleic acid aptasensor.The three new detection technology based on the electrochemical nucleic acid aptasensor,optical nucleic acid aptasensor and PCR nucleic acid aptasensor had advantages and disadvantages.A new idea in which glucose meter was used as a detection instrument and combined with nucleic acid aptasensor for AFB1 qualitative analysis were to be experimental veried.Combining nucleic acid aptasensor with magnetic nanoparticle united with bio-recognition elements as the adsorbent for solid phase extraction (SPE) was expected to be a breakthrough in the future.
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  • 收稿日期:  2016-09-01
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姜利英, 刘帅, 张培, 等. 基于核酸适体传感器的黄曲霉毒素B1检测研究进展[J]. 轻工学报, 2017, 32(2): 40-47. doi: 10.3969/j.issn.2096-1553.2017.2.007
引用本文: 姜利英, 刘帅, 张培, 等. 基于核酸适体传感器的黄曲霉毒素B1检测研究进展[J]. 轻工学报, 2017, 32(2): 40-47. doi: 10.3969/j.issn.2096-1553.2017.2.007
shu
JIANG Li-ying, LIU Shuai, ZHANG Pei, et al. Research progress of aflatoxin B1 detection based on nucleic acid aptasensor[J]. Journal of Light Industry, 2017, 32(2): 40-47. doi: 10.3969/j.issn.2096-1553.2017.2.007
Citation: JIANG Li-ying, LIU Shuai, ZHANG Pei, et al. Research progress of aflatoxin B1 detection based on nucleic acid aptasensor[J]. Journal of Light Industry, 2017, 32(2): 40-47. doi: 10.3969/j.issn.2096-1553.2017.2.007
shu

基于核酸适体传感器的黄曲霉毒素B1检测研究进展

  • 郑州轻工业学院 电气信息工程学院, 河南 郑州 450002
  • 收稿日期: 2016-09-01
  • 网络出版日期: 2017-03-15
基金项目:  国家自然科学基金项目（61002007）；河南省科技创新人才计划项目（124100510001）

摘要: 食物中存在毒性和致癌性较强的黄曲霉毒素B1（AFB1），目前常规的检测方法有薄层色谱法、高效液相色谱法、液相色谱-串联质谱法和免疫分析法，但都存在一定局限性.基于核酸适体传感器的AFB1检测研究取得了一定成果，基于电化学核酸适体传感器、光学核酸适体传感器和PCR核酸适体传感器的3种新型检测技术各有利弊，将血糖仪作为检测仪器、与核酸适体传感器相结合检测AFB1的思路有待实验验证.未来基于生物识别元件和磁性材料的磁亲和固相萃取法与核酸适体传感器相结合进行AFB1检测，有望获得突破.

English Abstract

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