石斛多糖超高压提取工艺条件的优化
Technique condition optimization for ultra high pressure extraction of dendrobium polysaccharide
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摘要: 采用超高压方法提取铁皮石斛中的多糖成分.在单因素实验的基础上,选取压力、时间、粉碎度和固液比(g:mL)4个影响因素,以石斛多糖为指标,通过正交试验优化超高压方法提取石斛多糖的工艺条件.结果表明,将石斛粉碎到80目后,按固液比1:20,采用300 MPa的压力提取6min后,石斛多糖的得率达到19.27%.这表明超高压方法是一有效的提取石斛多糖的方法.Abstract: Dendrobium candidum polysaccharide was extracted by ultra high pressure extraction (UHPE) method.Based on single factor experiments,orthogonal test was used to optimize the extraction conditions.Pressure,holding time,size and ratio of solid to liquid (g:mL) were as 4 factors and the yield of dendrobium polysaccharide (DP) was as target.The optimum parameters for extracting DP were as follows:pressure 300 MPa,holding time 6 min,size 80 mesh and ratio of solid to liquid (g:mL) 1:20.The yield of DP was 19.27%.So UHPE was a suitable method for extraction of DP.
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[1]
刘莉,萧凤回.石斛属药用植物多糖研究进展[J].现代中药研究与实践,2009,23(1):77.
-
[2]
罗傲霜,淳泽,葛绍荣,等.迭鞘石斛多糖降血糖作用研究[J].应用与环境生物学报,2006,12(3):334.
-
[3]
罗傲雪,宋关斌,淳泽,等.迭鞘石斛抗肿瘤作用研究[J].应用与环境生物学报,2007,13(2):184.
-
[4]
郝杰,查学强,鲍素华,等.霍山石斛不同分子量多糖体外抗氧化研究[J].食品科学,2009,30(15):94.
-
[5]
邓银华,徐康平,谭桂山.石斛属植物化学成分与药理活性研究进展[J].中药材,2002,25(9):677.
-
[6]
范益军,何兴金,何涛,等.正交设计优选金钗石斛多糖提取工艺研究[J].安徽农业科学,2009,37(23):10999.
-
[7]
周振鸣,来平凡.铁皮石斛多糖的超声提取工艺研究[J].中外健康文摘,2008,5(5):100.
-
[8]
Zhang S Q,Zhu J J,Wang C Z.Novel high pressure extraction technology[J].Int J of Pharmaceutics,2004,278:471.
-
[9]
钟先锋,黄桂东,邓泽元,等.荷叶多糖提取工艺的研究[J].食品与机械,2007,23(1):87.
-
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