JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 35 Issue 2
April 2020
Article Contents
HUANG Shen, MA Ning, WANG Qiongbo, et al. Screening, identification and fermentation optimization of flavor-enhancing microbial from reconstituted tobacco concentrate[J]. Journal of Light Industry, 2020, 35(2): 33-41. doi: 10.12187/2020.02.005
Citation: HUANG Shen, MA Ning, WANG Qiongbo, et al. Screening, identification and fermentation optimization of flavor-enhancing microbial from reconstituted tobacco concentrate[J]. Journal of Light Industry, 2020, 35(2): 33-41. doi: 10.12187/2020.02.005 shu

Screening, identification and fermentation optimization of flavor-enhancing microbial from reconstituted tobacco concentrate

  • Received Date: 2019-10-16
  • A flavor-enhancing strain HS-1, was screened and isolated from the reconstituted tobacco concentrate by dilute coating plate method.The strain was identified and the culture conditions for fermentation of the strain were optimized. The neutral aroma components of the reconstituted tobacco concentrate fermented under the optimal culture conditions were analyzed. The results showed that HS-1 was Planococcus sp.,the optimal culture conditions for fermentation were fermentation temperature of 30℃, inoculation amount of 5%, and fermentation time of 36 h. Under this culture condition, the total content of neutral aroma components in the reconstituted tobacco concentrate fermented by HS-1 was increased from 56.845% before fermentation to 72.527%, and the types and contents of neutral aroma components had increased. 5-methylfurfural and β-ionone were new ingredients after fermentation, the components such as benzyl alcohol, phenylacetaldehyde, 2-acetylpyrrole, phenethyl alcohol, solanone,β-damascenone, dihydroactinidiolide and megastigmatrienone had increased in varying degrees after fermentation.
  • 加载中
    1. [1]

      吴宇航,李思东.造纸法烟草薄片的研究进展[J].广东化工,2012,39(5):90.

    2. [2]

      戴路,陶丰,袁凯龙,等.造纸法再造烟叶的研究进展[J].中国造纸学报,2013,28(1):65.

    3. [3]

      李丹,刘熙.生物技术应用于造纸法再造烟叶生产的研究进展[J].现代食品科技,2013,29(6):1463.

    4. [4]

      闫克玉.烟草化学[M].郑州:郑州大学出版社,2002.

    5. [5]

      韩文佳,赵传山.造纸法烟草薄片发展现状[J].黑龙江造纸,2007(4):47.

    6. [6]

      程昌合,吴继忠,廖付,等.浓缩液醇化处理对烟草薄片致香成分及感官质量的影响[J].安徽农学通报,2011,17(1):142.

    7. [7]

      WANG W S,WANG Y,YANG L J,et al.Studies on thermal behavior of reconstituted tobacco sheet[J].Thermochimica Acta,2005,437(1/2):7.

    8. [8]

      LIU H G,HE H L,CHENG C H,et al.Diversity analysis of the bacterial community in tobacco waste extract during reconstituted tobacco process[J].Applied Microbiology and Biotechnology,2015,99(1):469.

    9. [9]

      周瑾,李雪梅,许传坤,等.利用微生物发酵改良烤烟碎片品质的研究[J].烟草科技,2002(6):3.

    10. [10]

      郑勤安.造纸法再造烟叶生产过程中微生物增质剂的应用研究[J].浙江工业大学学报,2004,32(4):442.

    11. [11]

    12. [12]

      张晨,许赣荣,严新龙.利用酿酒酵母改进烟草萃取液的香气特性[J].食品工业科技,2012,33(20):137.

    13. [13]

      贾蓓蕾,魏涛,黄申,等.α-胡萝卜素降解产香菌株的分离、鉴定及发酵条件优化[J].食品与发酵工业,2015,41(1):34.

    14. [14]

      吴丽君,段佳,李春子,等.同时蒸馏萃取-气相色谱/质谱法分析烟草中挥发性成分[J].分析科学学报,2012,28(6):807.

    15. [15]

      李炎强,冼可法.同时蒸馏萃取法与水蒸气蒸馏法分离分析烟草挥发性、半挥发性中性成分的比较[J].烟草科技,2000(2):18.

    16. [16]

      许甜甜.苯酚降解菌的分离鉴定及苯酚降解的相关研究[D].上海:上海师范大学,2012.

    17. [17]

      赖炜扬,林凯,鹿洪亮,等.再造烟叶正交优化提取及其化学成分和致香成分分析[J].厦门大学学报(自然科学版),2016,55(1):144.

    18. [18]

      ZHU X L,SU Q D,CAI J B,et al.Optimization of microwave-assisted solvent extraction for volatile organic acids in tobacco and its comparison with conventional extraction methods[J].Analytica Chimica Acta,2006,579(1):88.

Article Metrics

Article views(3310) PDF downloads(20) Cited by()

Ralated
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return