JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 35 Issue 6
December 2020
Article Contents
WANG Guanglu, ZHANG Fan, ZHOU Yifei, et al. Effect of site-specific mutagenesis of glycerol kinase coding gene on the glycerol utilization of Bacillus subtilis[J]. Journal of Light Industry, 2020, 35(6): 1-8. doi: 10.12187/2020.06.001
Citation: WANG Guanglu, ZHANG Fan, ZHOU Yifei, et al. Effect of site-specific mutagenesis of glycerol kinase coding gene on the glycerol utilization of Bacillus subtilis[J]. Journal of Light Industry, 2020, 35(6): 1-8. doi: 10.12187/2020.06.001 shu

Effect of site-specific mutagenesis of glycerol kinase coding gene on the glycerol utilization of Bacillus subtilis

  • Received Date: 2020-06-06
  • By employing a site-specific mutagenesis strategy for glycerol kinase (encoding by glpK), the active-site residue Met270 was replaced with Ile in the Bacillus subtilis genome. A mutant strain Bacillus subtilis M270I was constructed successfully by using the markerless gene replacement system. The results of growth analysis indicated that the mutant strain got a higher level of glycerol utilization. Compared with the control strain Bacillus subtilis 168 Δupp, the mutant strain exhibited higher specific growth rate (increased by 11%) and higher biomass yield (improved by 16%) with a shorten lag phase (shorten 2~4 h) in the M9 glycerol minimal medium.
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