包包曲与平板曲理化指标及细菌群落结构研究

刘延波; 王一菲; 赵志军; 韩素娜; 王贤; 孙西玉; 潘春梅

doi:10.12187/2021.06.002

December 2021

Article Contents

LIU Yanbo, WANG Yifei, ZHAO Zhijun, et al. Study on the physicochemical indexes and bacterial community structure of bag Daqu and brick Daqu[J]. Journal of Light Industry, 2021, 36(6): 9-20. doi: 10.12187/2021.06.002

Citation: LIU Yanbo, WANG Yifei, ZHAO Zhijun, et al. Study on the physicochemical indexes and bacterial community structure of bag Daqu and brick Daqu[J]. Journal of Light Industry, 2021, 36(6): 9-20. doi: 10.12187/2021.06.002 shu

Study on the physicochemical indexes and bacterial community structure of bag Daqu and brick Daqu

LIU Yanbo ^1,2 ,
WANG Yifei ¹ ,
ZHAO Zhijun ¹ ,
HAN Suna ² ,
WANG Xian ³ ,
SUN Xiyu ^1,4 ,
PAN Chunmei ¹

1. College of Food and Biological Engineering(Liquor College)/He'nan Liquor Style Engineering Technology Research Center/Zhengzhou Key Laboratory of Liquor Brewing Microbial Technology, He'nan University of Animal Husbandry and Economy, Zhengzhou 450046, China;
2. Postdoctoral Programme, He'nan Yangshao Liquor Co., Ltd., Mianchi 472400, China;
3. SheDianLaoJiu Co., Ltd., Sheqi 473300, China;
4. He'nan ZhangGongLaoJiu Wine Co., Ltd., Ningling 476733, China

Received Date: 2021-03-29

Abstract

The physicochemical indexes of bag Daqu and brick Daqu were studied. With high-throughput sequencing technology the bacterial community structure of bag Daqu and brick Daqu samples were analyzed. The results showed that the moisture content, acidity, starch content and other physicochemical indexes of brick Daqu and bag Daqu were within the standard range of finished Daqu. Compared with bag Daqu, the moisture content and fermentation power of brick Daqu were higher and the difference was significant;the saccharification power, liquefaction power, esterification power and fermentation power brick Daqu were higher, the acidity and starch content of brick Daqu were lower and the difference was very significant;the starch content was not much different. With the average relative abundance of 0. 05% as the threshold, the dominant bacteria of the bag Daqu and brick Daqu were Proteobacteria, Firmicutes and Actinobacteria. There was one more Bacteroidetes in the dominant bacteria of he brick Daqu than in bag Daqu. With the average relative abundance of 1. 00% as the threshold, the predominant bacterial genus of bag Daqu were Pseudomonas, Pantoea, Acinetobacter, Leuconostoc, Lactobacillus, and the predominant bacterial genus of brick Daqu were Lactobacillus, Leuconostoc, Pantoea, Klebsiella, Pseudomonas, Staphylococcus, Lactococcus. In this study, Barnesiella was discovered from the brick Daqu for the first time.
- bag Daqu,
- brick Daqu,
- physicochemical indexes,
- high-throughput sequencing,
- bacterial community structure
References
1. [1]
  李斌,闫志鹏,李慧星,等. 基于高通量测序技术的浓香型和芝麻香型白酒酒曲细菌群落结构分析[J]. 中国酿造,2018,37(8):148.
2. [2]
  LIU J J,CHEN J Y,FAN Y,et al. Biochemical characterisation and dominance of different hydrolases in different types of Daqu:a Chinese industrial fermentation starter[J]. Journal of the Science of Food and Agriculture,2018,98(1):113.
3. [3]
  施思,彭智辅,乔宗伟,等. 浓香型大曲贮藏过程中糖化力发酵力变化及真菌多样性分析[J]. 食品与发酵工业,2017,43(5):76.
4. [4]
  LI H,LIAN B,DING Y H,et al. Bacterial diversity in the central black component of Maotai Daqu and its flavor analysis[J]. Annals of microbiology,2014,64(4):1659.
5. [5]
  马加军,吕月明. 包包曲生产工艺条件控制[J]. 酿酒科技,2011(11):92.
6. [6]
  吕云怀,王莉,汪地强,等. 不同香型白酒大曲风味物质与其产品风格特征关系的分析[J]. 酿酒科技,2012(7):72.
7. [7]
  何宏魁,李兰,汤有宏,等. 古井贡酒大曲感官评价与其微生物、理化指标的关系研究[J]. 酿酒,2017,44(1):58.
8. [8]
  沈才洪,张良,应鸿,等. 大曲质量标准体系设置的探讨[J]. 酿酒科技,2005(11):8.
9. [9]
  谭崇尧,徐军,王令. 对枝江大曲不同工艺曲的质量研究[J]. 酿酒科技,2009(2):58.
10. [10]
  吕亚楠,沙均响. 不同培养时期对中高温大曲理化指标影响的研究[J]. 酿酒, 2017, 44(6):67.
11. [11]
  LIU P H,ZHANG L H,DU X W,et al. Dynamic analysis of physicochemical and biochemical indices and microbial communities of lightflavor Daqu during storage[J]. Journal of the American Society of Brewing Chemists,2019,77(4):287.
12. [12]
  WANG C L,SHI D J,GONG G L. Microorganisms in Daqu:a starter culture of Chinese Maotai-flavor liquor[J]. World Journal of Microbiology and Biotechnology,2008,24(10):2183.
13. [13]
  FAN G S,SUN B G,FU Z L,et al. Analysis of physicochemical indices, volatile flavor components, and microbial community of a light-flavor Daqu[J]. Journal of the American Society of Brewing Chemists, 2018,76(3):209.
14. [14]
  ZHANG H M,HE H K,YU X J,et al. Employment of near full-length ribosome gene TAcloning and primer-blast to detect multiple species in a natural complex microbial community using species-specific primers designed with their genome sequences[J]. Molecular Biotechnology,2016,58(11):729.
15. [15]
  张双燕,廖永红,纪南,等. 基于高通量测序技术分析北京清香型大曲微生物多样性[J]. 中国酿造,2016,35(11):49.
16. [16]
  王勇,周森,魏金旺. 基于高通量测序技术分析牛栏山大曲微生物多样性[J]. 中国酿造, 2019,38(2):58.
17. [17]
  WANG X D,BAN S D,HU B D,et al. Bacterial diversity of Moutai-flavour Daqu based on highthroughput sequencing method[J]. Journal of the Institute of Brewing, 2017,123(1):138.
18. [18]
  王洪,罗惠波,周平,等. 热风干燥大曲在贮存期细菌群落结构的变化[J]. 现代食品科技, 2017,33(11):89.
19. [19]
  戴奕杰,李宗军,田志强. 酱香型白酒大曲和糟醅的细菌多样性分析[J]. 食品科学,2019, 40(4):152.
20. [20]
  马茹菲,马玉帛,佟世生,等. 不同清香型大曲理化生化指标和挥发性成分分析[J]. 酿酒科技,2019(3):70.
21. [21]
  中华人民共和国工业和信息化部. 酿酒大曲通用分析方法:QB/T 4257-2011[S]. 北京:中国标准出版社,2011.
22. [22]
  中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会. 白酒分析方法:GB/T 10345-2007[S]. 北京:中国标准出版社,2007.
23. [23]
  张丽,卢红梅,陈莉,等. 酱香型大曲贮存过程中发酵性能变化的研究[J]. 现代食品科技, 2017,33(9):231.
24. [24]
  李超,穆琳,王建耀,等. 汾型大曲的理化指标和微生物指标分析[J]. 中国酿造,2009(1):140.
25. [25]
  李媛媛,罗碧霞,张兰兰,等. 不同粮食曲的理化特性及其微生物群落结构研究[J]. 食品与发酵工业,2020,46(10):74.
26. [26]
  涂荣坤,钱志伟,秦辉,等. 大曲检测指标研究进展[J]. 酿酒科技,2016(1):110.
27. [27]
  CHAO A,JOST L,HSIEH T C,et al. Expected Shannon entropy and Shannon differentiation between subpopulations for neutral genes under the finite island model[J]. PLOS ONE, 2015, 10(6):1.
28. [28]
  ERCOLINI D. High-throughput sequencing and metagenomics:moving forward in the cultureindependent analysis of food microbial ecology[J]. Applied and Environmental Microbiology, 2013, 79(10):3148.
29. [29]
  陶寅亮. 北方中高温包包曲制曲过程关键控制点分析[J]. 现代食品,2016(3):120.
30. [30]
  雷学俊,张霞,刘多涛,等. 包包曲及制曲环境中可培养霉菌的分离与鉴定[J]. 酿酒科技, 2019(12):17.
31. [31]
  陈玲,曾丽云,袁玉菊,等. 浓香型白酒大曲与入窖糟醅细菌多样性的对比分析[J]. 酿酒科技,2015(10):39.
32. [32]
  姚粟,葛媛媛,李辉,等. 利用非培养技术研究芝麻香型白酒高温大曲的细菌群落多样性[J]. 食品与发酵工业,2012,38(6):1.
33. [33]
  吴树坤,谢军,卫春会,等. 四川不同地区浓香型大曲微生物群落结构比较[J]. 食品科学, 2019,40(14):144.
34. [34]
  梁晨,杜海,徐岩. 大曲贮存过程中原核微生物群落结构及风味成分演替规律[J]. 微生物学通报,2017,44(2):384.
35. [35]
  康承霞,张宿义,马蓉,等. 浓香型白酒大曲微生物研究进展[J]. 酿酒科技,2017(5):88.
36. [36]
  LI B O, XIN G, NA L, et al. Fermentation process of mulberry juice-whey based Tibetan kefir beverage production[J]. Czech Journal of Food Sciences, 2019, 36(6):494.
37. [37]
  翟磊,信春晖,许玲,等. 芝麻香型白酒高温大曲产脂肪酶菌株的筛选与鉴定[J]. 食品与发酵工业,2015,41(12):36.
38. [38]
  黄丹,张强,严芳,等. 浓香型大曲中酯化酶细菌的分离鉴定及产酶条件研究[J]. 中国酿造,2009(8):57.
39. [39]
  孟天毅,李长文,山其木格,等. 酱香强化大曲发酵过程中微生物的变化[J]. 酿酒科技, 2015(5):65.
40. [40]
  陈可丹,吴晓江,陈延儒,等. 顶温对特香型大曲理化指标及菌群演替的影响[J]. 食品与发酵工业,2020,46(5):52.
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Study on the physicochemical indexes and bacterial community structure of bag Daqu and brick Daqu

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