动物肝酯酶对西维因的抑制响应

王君; 袁亚明; 江晶晶; 许慧卿; 李松南

doi:10.12187/2022.03.002

June 2022

Article Contents

WANG Jun, YUAN Yaming, JIANG Jingjing, et al. Inhibitory response to carbaryl of animal liver esterase[J]. Journal of Light Industry, 2022, 37(3): 10-16. doi: 10.12187/2022.03.002

Citation: WANG Jun, YUAN Yaming, JIANG Jingjing, et al. Inhibitory response to carbaryl of animal liver esterase[J]. Journal of Light Industry, 2022, 37(3): 10-16. doi: 10.12187/2022.03.002 shu

Inhibitory response to carbaryl of animal liver esterase

1. School of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, China;
2. Institutes of Agricultural Science and Technology Development/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China

Received Date: 2021-08-29
Accepted Date: 2022-01-21

Abstract

Through graded salting out with ammonium sulfate and dialysis, esterases were extracted and purified from goose liver, chicken liver and pig liver respectively, then their structures were characterized, and their inhibition responses to carbaryl as a pesticide model were further investigated. Results showed that the yield of goose liver esterase was the highest (14.58%), and that of chicken liver esterase was the lowest (8.82%) with the best thermal stability, while the protein mass concentration of pig liver esterase was the highest (12.47 mg/mL); the protein molecular weights of all three kinds of animal liver esterases were 30~110 kDa with the leading one of 55 kDa, and that in chicken liver esterase was the highest (16.74%) while the lowest was in that of pig liver esterase (14.31%); these three kinds of animal liver esterases showed different sizes of protein particles with flake structures and smooth surfaces; in the carbaryl concentration range of 0.5~10.0 mg/L, the linear range (0.5~5.0 mg/L), the detection limit (0.29 mg/L) and IC50 (7.74 mg/L) of chicken liver esterase were the lowest, which could be used as a potential sensitive enzyme source for rapid detection of carbaryl.
- carbaryl,
- animal liver esterase,
- enzyme-linked immunosorbent assay,
- detection of agricultural residues
References
1. [1]
  陈斌,吴芳芳,周翔,等.果蔬食品安全问题研究及对策分析[J].食品安全导刊,2020,294(35):71-73.
2. [2]
  陈周秀,罗影,罗杨丽.农产品中农药残留检测技术研究进展[J].农产品加工,2020,497(3):70-73.
3. [3]
  周邦萌,李其美.酶联免疫吸附分析技术在食品农药残留检测中的应用探究[J].食品安全导刊,2020,277(18):87-88.
4. [4]
  朱松明,周晨楠,和劲松,等.基于酶抑制法的农药残留快速比色检测[J].农业工程学报,2014,30(6):242-248.
5. [5]
  陈子鹏,马建博,杜建华.基于酶抑制法的自助式农药残留检测平台建立[J].农业工程学报,2019,35(21):289-294.
6. [6]
  张佳佳.乙酰胆碱酯酶酶源的筛选、提取及其特性研究[D].广州:华南理工大学,2016.
7. [7]
  王琳琳,高兴明,韦海涛,等.固定化酶在食品工业中的应用研究进展[J].轻工学报,2021,36(2):25-33.
8. [8]
  徐斐,许学勤,张慧君,等.鸡肝酯酶在有机磷和氨基甲酸酯类农药检测中的应用研究[J].食品科学,2007,330(5):237-240.
9. [9]
  杨莉,王羚佳,开拓,等.猪肝酯酶农药敏感性及其与胆碱酯酶的对比研究[J].西南农业学报,2018,31(7):1536-1542.
10. [10]
  孙克江,张甜,曲梅丽,等.聚丙烯酰胺凝胶电泳法快速检测植物源性食品中的蛋白成分[J].食品安全质量检测学报,2017,8(3):895-899.
11. [11]
  许可,邱国栋,李星科,等.冻藏时间对面团水分物态变化及品质特性的影响[J].轻工学报,2021,36(1):9-16.
12. [12]
  于晓航,朱念辛,徐斐,等.分离纯化提高鸡肝酯酶对农药抑制响应的研究[J].现代食品科技,2015,31(6):230-235.
13. [13]
  朱小山,孟范平,何东海.鲅鱼脑组织AChE的分离纯化及某些生化性质[J].青岛大学学报(工程技术版),2006,21(2):35-41.
14. [14]
  叶东平.葱蝇乙酰胆碱酯酶的纯化及其生化毒理学特性研究[D].重庆:重庆师范大学,2011.
15. [15]
  BÖTTCHER D,BRUSEHABER E,DODERER K,et al.Functional expression of the γ-isoenzyme of pig liver carboxyl esterase in Escherichia coli[J].Applied Microbiology and Biotechnology,2007,73(6):1282-1289.
16. [16]
  陈珂,刘丽莉,孟圆圆,等.鸡蛋清蛋白喷雾干燥工艺优化及特性变化[J].食品与机械,2019,35(1):197-203
  ,231.
17. [17]
  李向红,王发祥,张付兰,等.冷藏过程中草鱼肌原纤维蛋白结构的变化[J].食品与发酵工业,2015,41(10):177-181.
18. [18]
  GHANGHAS N,PRABHAKAR P K,SHARMA S,et al.Microfluidization of fenugreek (Trigonella foenum graecum) seed protein concentrate:Effects on functional,rheological,thermal and microstructural properties[J].LWT-Food Science and Technology,2021,149:111830.
19. [19]
  REED Z H,PARK J W.Rheological and biochemical characterization of salmon myosin as affected by constant heating rate[J].Journal of Food Science,2011,76(2):343-349.
20. [20]
  金盛烨.酶抑制荧光探针法检测有机磷和氨基甲酸酯类农药的研究[D].大连:中国科学院研究生院(大连化学物理研究所),2004.
21. [21]
  杨莉,王羚佳,开拓,等.猪肝酯酶农药敏感性及其与胆碱酯酶的对比研究[J].西南农业学报,2018,31(7):1536-1542.
22. [22]
  何绍志,李维,王雁,等.美洲蟑螂头部乙酰胆碱酯酶酶活性测定及对5种常用农药的敏感性[J].食品科学,2013,468(23):184-188.
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沈阳化工大学材料科学与工程学院沈阳 110142

Inhibitory response to carbaryl of animal liver esterase

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