代谢工程改造枯草芽孢杆菌促进L-赖氨酸高效合成研究

张晓霞; 陈胜玲; 朱枝群; 康春涛; 徐建中

doi:10.12187/2022.05.001

Volume 37 Issue 5

October 2022

Article Contents

Abstract

References

ZHANG Xiaoxia, CHEN Shengling, ZHU Zhiqun, et al. Metabolic engineering for improving the L-lysine production by Bacillus subtilis[J]. Journal of Light Industry, 2022, 37(5): 1-11. doi: 10.12187/2022.05.001

Citation: ZHANG Xiaoxia, CHEN Shengling, ZHU Zhiqun, et al. Metabolic engineering for improving the L-lysine production by Bacillus subtilis[J]. Journal of Light Industry, 2022, 37(5): 1-11. doi: 10.12187/2022.05.001 shu

Metabolic engineering for improving the L-lysine production by Bacillus subtilis

1. Jiangsu Xinghai Biotechnology Co., Ltd., YanCheng 224233, China;
2. School of Biotechnology/The Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, WuXi 214122, China

Received Date: 2022-02-13

Abstract

In order to construct a dual-functional B.subtilis with probiotic function and L-lysine production, B.subtilis ACCC11025 was systematically modified. These results indicated that the strain with replacement of thrD,zwf and gnd from B.subtilis by lysC³¹¹,zwf²³⁴ and gnd³⁶¹ from C.glutamicum (i.e.,B.subtilis XH4) was beneficial to L-lysine production, and the yield of L-lysine was (20.3±1.9) g/L. In addition, the strain with replacement of hom from B.subtilis by hom⁵⁹ from C.glutamicum(i.e.,B.subtilis XH5)produced (23.2±1.7) g/L of L-lysine without the decrease of cell growth. In addition, the yield of by-products in B.subtilis XH5 was significantly decreased. Moreover, the DapDH from C.glutamicum was introduced into the B.subtilis XH5 (i.e, B.subtilis XH6), resulting in the increase of L-lysine production because of the redirection of the carbon flux in DAP pathway. The resulted recombinant strain B.subtilis XH6 produced (25.6±2.3) g/L of L-lysine.
- Bacillus subtilis,
- L-lysine production,
- CRISPR-Cas9,
- feedback regulation,
- metabolic engineering
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