基于芽孢杆菌固态发酵技术提高广西河池C4F烟叶品质的研究

薛云; 宁振兴; 苏赞; 刘启斌; 王敏; 毛多斌; 龙章德; 魏涛

doi:10.12187/2022.05.009

October 2022

Article Contents

XUE Yun, NING Zhenxing, SU Zan, et al. Study on improving the quality of Hechi C4F tobacco leaves in Guangxi by using the solid-state fermentation technology with Bacillus sp.[J]. Journal of Light Industry, 2022, 37(5): 76-84. doi: 10.12187/2022.05.009

Citation: XUE Yun, NING Zhenxing, SU Zan, et al. Study on improving the quality of Hechi C4F tobacco leaves in Guangxi by using the solid-state fermentation technology with Bacillus sp.[J]. Journal of Light Industry, 2022, 37(5): 76-84. doi: 10.12187/2022.05.009 shu

Study on improving the quality of Hechi C4F tobacco leaves in Guangxi by using the solid-state fermentation technology with Bacillus sp.

XUE Yun ¹ ,
NING Zhenxing ¹ ,
SU Zan ¹ ,
LIU Qibin ¹ ,
WANG Min ² ,
MAO Duobin ² ,
LONG Zhangde ¹ ,
WEI Tao ²

1. Technology Center, China Tobacco Guangxi Industrial Co., Ltd., Nanning 530004, China;
2. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Received Date: 2021-08-16

Abstract

C4F tobacco leaves in Hechi area, Guangxi were treated by solid-state fermentation with Bacillus sp. ZZ-8. Single factor test and response surface methodology experiment were used to obtain the best processing conditions of Guangxi tobacco leaves treated by Bacillus sp. ZZ-8. The smoking quality of treated tobacco leaves was evaluated by analyzing the content changes of conventional chemical components and important aroma components, combined with sensory evaluation technology. Research results demonstrated that the optimum extracting condition was as follow: inoculum 3 mL, fermentation temperature 28 ℃, fermentation time 36 h, and tobacco leaf relative humidity 60%. Under this condition, the highest content of petroleum ether extract was 9.01%.The total sugar content of tobacco leaves decreased from 23.10% to 22.20%, the reducing sugar content increased from 22.19% to 22.40%, and the sugar alkali ratio decreased from 10.23 to 9.45. Benzaldehyde and β-cyclocitral were produced in tobacco leaves after fermentation, the overall content of aroma components in C4F tobacco increased from 170.444 μg/g to 186.267 μg/g in which the content of aroma substances such as damascene, solanone, megastigmatrienone, dihydrodamascone, geranyl acetone and dihydroactinidide changed greatly. After sensory evaluation, the aroma quality of treated tobacco leaves increased, the irritation and miscellaneous gas decreased, the flue gas was fine and soft, the aftertaste was comfortable, and the smoking quality was improved.
- C4F tobacco leaves,
- Bacillus sp.ZZ-8,
- conventional chemical components,
- aroma components,
- solid-state fermentation
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Study on improving the quality of Hechi C4F tobacco leaves in Guangxi by using the solid-state fermentation technology with Bacillus sp.

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