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CN 41-1437/TS  ISSN 2096-1553

绿茶茶末多酚的提取、鉴定及其生物活性研究

汪雪莲 ,  冯慧祥 ,  薛世华 ,  谢譞 ,  刘鹏展

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汪雪莲, 冯慧祥, 薛世华, 等. 绿茶茶末多酚的提取、鉴定及其生物活性研究[J]. 轻工学报, 2022, 37(6): 58-67. doi: 10.12187/2022.06.008
引用本文: 汪雪莲, 冯慧祥, 薛世华, 等. 绿茶茶末多酚的提取、鉴定及其生物活性研究[J]. 轻工学报, 2022, 37(6): 58-67. doi: 10.12187/2022.06.008
shu
WANG Xuelian, FENG Huixiang, XUE Shihua, et al. Extraction,identification and bioactivities of polyphenols from green tea dust[J]. Journal of Light Industry, 2022, 37(6): 58-67. doi: 10.12187/2022.06.008
Citation: WANG Xuelian, FENG Huixiang, XUE Shihua, et al. Extraction,identification and bioactivities of polyphenols from green tea dust[J]. Journal of Light Industry, 2022, 37(6): 58-67. doi: 10.12187/2022.06.008
shu

绿茶茶末多酚的提取、鉴定及其生物活性研究

  • 华南理工大学 食品科学与工程学院, 广东 广州 510641

    • 作者简介: 汪雪莲(2000—),女,四川省达州市人,华南理工大学硕士研究生,主要研究方向为植物天然产物及食品加工。E-mail:wxuelian1006@163.com;

  • 基金项目: 广东省基础与应用基础研究基金项目(2021A1515012504)

  • 中图分类号: TS209

Extraction,identification and bioactivities of polyphenols from green tea dust

  • School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China

  • Received Date: 2022-05-18

    CLC number: TS209

  • 摘要: 以西湖龙井茶末为原料,以料液比1∶20(g/mL)、提取温度85 ℃、乙醇体积分数60%、提取时间60 min为提取条件对茶末中的多酚进行提取,采用4种不同极性有机溶剂萃取游离多酚,并对不同极性提取物中的总酚质量浓度、自由基清除能力、胰脂肪酶抑制率、α-淀粉酶抑制率等进行测定以研究其生物活性。结果表明:4种萃取相中,乙酸乙酯萃取相的总多酚质量浓度最高(15.66 mg/mL),且具有最强的生物活性,其对DPPH和ABTS自由基清除率的半抑制浓度(IC50)分别为 0.05 mg/mL和 0.15 mg/mL,对胰脂肪酶和α-淀粉酶抑制率的IC50分别为 0.89 mg/mL和0.39 mg/mL;液相色谱-质谱(LC-MS)定性分析共鉴定出15种多酚类化合物,且首次鉴定出根皮苷(5.44 mg/g);高效液相色谱(HPLC)对其中含量较高的化合物进行定量分析发现,表没食子儿茶素、儿茶素、绿原酸的含量较高,分别为72.03 mg/g、48.62 mg/g和41.58 mg/g。
    Abstract: Green tea dust was used as raw material in this study. The polyphenols in the green tea dust were extracted by a solid-liquid ratio of 1∶20 g/mL, extraction temperature of 85 ℃, ethanol concentration of 60% and extraction time of 60 min, and solvents with different polar were employed to extract and separate and the bioactive components in the green tea dust into 4 fractions. The mass concentration of total phenols contained in the different fractions was determined, and the bioactivates of different fractions were evaluated through free radical scavenging assay, and their inhibitory activities on pancreatic lipase and α-amylase were also studied. The results showed that the ethyl acetate extracted fraction had the highest total polyphenol content (15.66 mg/mL) and the strongest bioactivities among all the fractions. Its IC50 for the free radical scavenging rate of DPPH and ABTS were 0.05 mg/mL and 0.15 mg/mL, respectively, and inhibitory activities on pancreatic lipase and α-amylase were IC50 0.89 mg/mL and 0.39 mg/mL, respectively. The polyphenols in the ethyl acetate extracted phase were further characterized and quantified by HPLC and MS. A total of 15 polyphenolic compounds were identified by MS and was first identified of phlorizin(5.44 mg/g) in green tea dust. Quantitative analysis of the compounds with high contents by HPLC revealed that epigallocatechin, catechin and chlorogenic acid were present at high contents of 72.03 mg/g, 48.62 mg/g and 41.58 mg/g respectively.
    1. [1]

      LIU J Q,SHAO W F,HUANG Y W,et al.Effect of fermented Pu-erh tea powder, dark tea powder and liupu tea on hepatocytic CYP2E1 expression in rats with experimental nonalcoholic fatty liver[J].Chinese Journal of Gastroenterology,2013,18(7):411-415.

    2. [2]

      CHOU O,ALI A,SUBBIAH V,et al.LC-ESI-QTOF-MS/MS characterisation of phenolics in herbal tea infusion and their antioxidant potential[J].Fermentation-Basel,2021,7(2):73.

    3. [3]

      SAFFARI Y,SADRZDEH S M H.Green tea metabolite EGCG protects membranes against oxidative damage in vitro[J].Life Sciences,2004,74(12):1513-1518.

    4. [4]

      赵雅倩.典型酚类抗氧化剂对斑马鱼的神经毒性效应研究[D].呼和浩特:内蒙古大学, 2021.

    5. [5]

      ZHANG Z C,ZHANG Y T,LI J M,et al.The neuroprotective effect of tea polyphenols on the regulation of intestinal flora[J].Molecules, 2021,26(12):3692.

    6. [6]

      PRAMILA K,JULIUS A.In vitro antioxidant effect of green tea polyphenol epigallocatechin-3-gallate (EGCG) in protecting cardiovascular diseases[J].Research Journal of Pharmacy and Technology, 2019,12(3):1265-1267.

    7. [7]

      李新.氨基酸和酚类化合物的联合抗氧化作用研究[D].长春:吉林大学, 2017.

    8. [8]

      郑城钦,张正艳,马存强,等.茶多酚在日用品及食品中的应用[J].现代食品, 2020(17):64-67.

    9. [9]

      LORENZ M,LEHMANN S,DJORDJEVIC I,et al.Vasodilation of tea polyphenols ex vivo is mediated by hydrogen peroxide under rapid compound decay[J].Antioxidants,2020,9(5):390.

    10. [10]

      李元慈.茶多酚对肝脏的保护作用研究[J].中国食物与营养, 2019,25(9):75-78.

    11. [11]

      杨宽,钱卫东,秦蓓.茶多酚对高脂血症大鼠血脂代谢和肝组织MDA、T-SOD含量的影响[J].中国油脂,2019,44(1):70-73
      ,96.

    12. [12]

      张勇,李苗苗,华田苗,等.茶多酚对慢性酒精中毒大鼠肝损伤的保护作用[J].中国应用生理学杂志,2018,34(6):481-484
      ,529.

    13. [13]

      汪雪睿,黄保嘉,李想,等.茶多酚对急性镉(Ⅱ)暴露小鼠红细胞及肝脏损伤的拮抗作用[J].中国食品学报,2020,20(4):66-72.

    14. [14]

      WANG D X,WANG T T,LI Z M,et al.Green tea polyphenols upregulate the Nrf2 signaling pathway and suppress oxidative stress and inflammation markers in D-Galactose-Induced liver aging in mice[J].Frontiers in Nutrition,2022,9:836112.

    15. [15]

      李原,赵振刚.凤眼果壳多酚的抗氧化和抗人肺癌细胞A549增殖活性[J].现代食品科技,2021,37(4):16-24.

    16. [16]

      国家市场监督管理总局,中国国家标准化管理委员会.茶叶中茶多酚和儿茶素类含量的检测方法:GB/T 8313—2018[S].北京:中国标准出版社,2018.

    17. [17]

      ZHAO Z G,YAN H F,ZHENG R,et al.Anthocyanins characterization and antioxidant activities of sugarcane (Saccharum officinarum L.) rind extracts[J].Industrial Crops and Products,2018,113:38-45.

    18. [18]

      高超.款冬叶黄酮的抗氧化活性研究[J].轻工学报,2020,35(2):17-23.

    19. [19]

      ZHENG R,SU S,ZHOU H F,et al.Antioxidant/antihyperglycemic activity of phenolics from sugarcane (Saccharum officinarum L.) bagasse and identification by UHPLC-HR-TOFMS[J].Industrial Crops and Products,2017,101:104-114.

    20. [20]

      张煜,张晓娜,薛桥丽,等.扁枝槲寄生提取物体外抗氧化效应研究[J].轻工学报,2016,31(3):39-48.

    21. [21]

      张忠.茶多酚对胰脂肪酶活性的抑制作用[J].食品工业,2013,34(8):168-167.

    22. [22]

      王文君,姚江武,陶涛.红茶和绿茶多酚对猪胰腺α-淀粉酶抑制效果的动力学研究[J].口腔医学研究,2009,25(4):442-446.

    23. [23]

      ZHENG R,SU S,LI J B,et al.Recovery of phenolics from the ethanolic extract of sugarcane (Saccharum officinarum L.) baggase and evaluation of the antioxidant and antiproliferative activities[J].Industrial Crops and Products,2017,107:360-369.

    24. [24]

      MONIRUZZAMAN M,DUTTA S D,JIN H X,et al.Polyphenol derived bioactive carbon quantum dot-incorporated multifunctional hydrogels as an oxidative stress attenuator for antiaging and in vivo wound-healing applications[J].Biomaterials Science,2022,13(10):3527-3539.

    25. [25]

      ISWANTINI D,HANIF N,RIZKI P D,et al.Active fraction as anti-obesity by in vitro toward pancreatic lipase activity[J].IOP Conference Series Materials Science and Engineering,2021,1070(1):12012.

    26. [26]

      LI S,PAN J H,HU X,et al.Kaempferol inhibits the activity of pancreatic lipase and its synergistic effect with orlistat[J].Journal of Functional Foods,2020,72:104041.

    27. [27]

      刘天囡,徐梦佳,胡冰,等.茯砖茶多酚类物质对胰脂肪酶活性的抑制作用[J].食品科学,2015,36(21):46-49.

    28. [28]

      SOARES S,MATEUS N,DE FREITAS V.Interaction of different polyphenols with bovine serum albumin (BSA) and human salivary alpha-amylase (HSA) by fluorescence quenching[J].Journal of Agricultural and Food Chemistry,2007,55(16):6726-6735.

    29. [29]

      INGLETT G,DAVID S.Alpha-amylase activity in various concentrations of the ionic liquid,1-butyl-3-methylimidazolium chloride[J].Agricultural Research Service,2008,8(3):58.

    30. [30]

      FEJERSKOV O,KIDD E.Dental caries:The disease and its clinical management[J].Community Dentistry and Oral Epidemiology,2004,32:236-237.

    31. [31]

      NARAYANAN R,CHANDRAN R,PRASUNKUMAR J M.Human salivary α-amylase trp58 situated at subsite-2 is critical for enzyme activity[J].European Journal of Biochemistry,2004,271(12):2517-2529.

    32. [32]

      王文君,姚江武,陶涛.红茶和绿茶多酚对猪胰腺α-淀粉酶抑制效果的动力学研究[J].口腔医学研究, 2009,25(4):442-446.

    33. [33]

      张丽娜.水翁花对胰脂肪酶和α-淀粉酶抑制活性及作用机制初步探讨[D].上海:华东理工大学,2003.

    34. [34]

      FANG N B,YU S G,PRIOR R L.LC/MS/MS characterization of phenolic constituents in dried plums[J].Agricultural and Food Chemistry, 2002,50(12):3579-3585.

    35. [35]

      SHOKO T,MAHARAJ V J,NAIDOO D,et al.Anti-aging potential of extracts from sclerocarya birrea (A.Rich.) hochst and its chemical profiling by UPLC-Q-TOF-MS[J].BMC Complementary and Alternative Medicine,2018,18:54.

    36. [36]

      DANIIL O,CHIRIKOVA N K,KASHCHENKO N I,et al.Bioactive phenolics of the genus artemisia (Asteraceae):HPLC-DAD-ESI-TQ-MS/MS profile of the siberian species and their inhibitory potential against α-Amylase and α-Glucosidase[J].Frontiers in Pharmacology,2018,9:756.

    37. [37]

      金钰,赵明明,周有祥,等.龙井茶中主要茶多酚的溶出行为研究[J].湖北农业科学,2015,54(23):6007-6011.

    38. [38]

      LIU P Z,YANG B R,KALLIO H.Characterization of phenolic compounds in chinese hawthorn (crataegus pinnatifida bge.var.major) fruit by high performance liquid chromatography-electrospray ionization mass spectrometry[J].Food Chemistry,2010,121(4):1188-1197.

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汪雪莲, 冯慧祥, 薛世华, 等. 绿茶茶末多酚的提取、鉴定及其生物活性研究[J]. 轻工学报, 2022, 37(6): 58-67. doi: 10.12187/2022.06.008
引用本文: 汪雪莲, 冯慧祥, 薛世华, 等. 绿茶茶末多酚的提取、鉴定及其生物活性研究[J]. 轻工学报, 2022, 37(6): 58-67. doi: 10.12187/2022.06.008
shu
WANG Xuelian, FENG Huixiang, XUE Shihua, et al. Extraction,identification and bioactivities of polyphenols from green tea dust[J]. Journal of Light Industry, 2022, 37(6): 58-67. doi: 10.12187/2022.06.008
Citation: WANG Xuelian, FENG Huixiang, XUE Shihua, et al. Extraction,identification and bioactivities of polyphenols from green tea dust[J]. Journal of Light Industry, 2022, 37(6): 58-67. doi: 10.12187/2022.06.008
shu

绿茶茶末多酚的提取、鉴定及其生物活性研究

    作者简介:汪雪莲(2000—),女,四川省达州市人,华南理工大学硕士研究生,主要研究方向为植物天然产物及食品加工。E-mail:wxuelian1006@163.com
  • 华南理工大学 食品科学与工程学院, 广东 广州 510641
  • 收稿日期: 2022-05-18
基金项目:  广东省基础与应用基础研究基金项目(2021A1515012504)

摘要: 以西湖龙井茶末为原料,以料液比1∶20(g/mL)、提取温度85 ℃、乙醇体积分数60%、提取时间60 min为提取条件对茶末中的多酚进行提取,采用4种不同极性有机溶剂萃取游离多酚,并对不同极性提取物中的总酚质量浓度、自由基清除能力、胰脂肪酶抑制率、α-淀粉酶抑制率等进行测定以研究其生物活性。结果表明:4种萃取相中,乙酸乙酯萃取相的总多酚质量浓度最高(15.66 mg/mL),且具有最强的生物活性,其对DPPH和ABTS自由基清除率的半抑制浓度(IC50)分别为 0.05 mg/mL和 0.15 mg/mL,对胰脂肪酶和α-淀粉酶抑制率的IC50分别为 0.89 mg/mL和0.39 mg/mL;液相色谱-质谱(LC-MS)定性分析共鉴定出15种多酚类化合物,且首次鉴定出根皮苷(5.44 mg/g);高效液相色谱(HPLC)对其中含量较高的化合物进行定量分析发现,表没食子儿茶素、儿茶素、绿原酸的含量较高,分别为72.03 mg/g、48.62 mg/g和41.58 mg/g。

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