JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 38 Issue 2
April 2023
Article Contents
LI Ke, WANG Linmeng, GAO Huijian, et al. Physicochemical properties of ultrasound-assisted alkali extraction treated PSE-like chicken protein isolate-EGCG composite system[J]. Journal of Light Industry, 2023, 38(2): 14-23,62. doi: 10.12187/2023.02.002
Citation: LI Ke, WANG Linmeng, GAO Huijian, et al. Physicochemical properties of ultrasound-assisted alkali extraction treated PSE-like chicken protein isolate-EGCG composite system[J]. Journal of Light Industry, 2023, 38(2): 14-23,62. doi: 10.12187/2023.02.002 shu

Physicochemical properties of ultrasound-assisted alkali extraction treated PSE-like chicken protein isolate-EGCG composite system

  • Received Date: 2022-10-09
  • In order to improve the utilization of PSE (Pale, Soft and Exudative)-like chicken protein isolate and to prevent the oxidative degradation of epigallocatechin-3-gallate (EGCG), the effects of alkali solubilization andextraction followed by acid depositionprecipitation method and ultrasound (20 kHz, 450 W, 10 min) assisted alkali methodextraction (UAE) on the structure of PSE-like chicken protein isolate and their protection on EGCG were analyzed. The results showed that the average particle size and turbidity of PSE-like chicken protein isolate obtained by UAE decreased and the absolute value of the potential increased compared to the alkali extraction followed by acid precipitation treatment, the average particle size of the composite system was significantly decreased after the addition of EGCG (Pα-helix and β-sheet of PSE-like chicken protein isolate by UAE-treated were decreased to 12.84% and 11.96%, respectively, while the relative content of β-turn was increased to 63.17% (Pα-helix decreased significantly to 9.07% and the relative content of β-sheet increased significantly to 19.08% after the addition of EGCG (P<0.05). The PSE-like chicken protein isolate obtained by UAE had the stronger binding ability to EGCG, with a larger binding site (1.265), and better protective effect on EGCG. UAE altered the structure of PSE-like chicken protein isolate, enhanced the interaction of PSE chicken protein isolate with EGCG, and slowed down the oxidative degradation of EGCG.
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