基于氧化香菇多糖金纳米簇复合物的电化学发光在腐胺检测中的应用

刘星洁; 侯娟; 田数; 贾学伟; 李天笑; 许春平

doi:10.12187/2023.05.005

October 2023

Article Contents

LIU Xingjie, HOU Juan, TIAN Shu, et al. Application of electrochemiluminescence based on oxidized lentinan Au nanocluster composites in the detection of putrescine[J]. Journal of Light Industry, 2023, 38(5): 36-41,67. doi: 10.12187/2023.05.005

Citation: LIU Xingjie, HOU Juan, TIAN Shu, et al. Application of electrochemiluminescence based on oxidized lentinan Au nanocluster composites in the detection of putrescine[J]. Journal of Light Industry, 2023, 38(5): 36-41,67. doi: 10.12187/2023.05.005 shu

Application of electrochemiluminescence based on oxidized lentinan Au nanocluster composites in the detection of putrescine

LIU Xingjie ^1,2 ,
HOU Juan ^1,2 ,
TIAN Shu ³ ,
JIA Xuewei ⁴ ,
LI Tianxiao ⁴ ,
XU Chunping ⁴

1. College Research Center for Energy Saving and Emissions Reduction of Electric Power of Henan Province, Zhengzhou 450000, China;
2. Zhengzhou Electric Power College, Zhengzhou 450000, China;
3. Inner Mongolia Kunming Cigarettes Co., Ltd., Hohhot 010020, China;
4. College of Food Tobacco Science and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Received Date: 2022-07-16
Accepted Date: 2023-03-21

Abstract

Lentinan (LNT) was pre-oxidation and polysaccharide/Au nanoclusters (OLNT-AuNCs) were prepared using oxidized polysaccharides (OLNT) via microwave-assisted synthesis. Based on the quenching effect of putrescine on the electrochemiluminescence (ECL) of OLNT-AuNCs, a putrescine sensor was established and used to detect the mass concentration of putrescine in three commercially-available dry red wines. The results showed that OLNT had a good dispersion effect on AuNCs, the average particle size of AuNCs was 2.8 nm, and the spacing of lattice stripes was 0.23 nm. Under UV irradiation, AuNCs emitted red fluorescence with a peak of 612 nm; OLNT-AuNCs were excellent ECL emitters in the co-reaction reagents K₂S₂O₈ system, putrescine had stable and specific quenching effect on this ECL system, and the decreased ECL intensity was linear with the logarithm of concentrations of putrescine in the range of 0.03~18.6 mg/L (R²=0.997 5), and the limit of detection was 9 μg/L (S/N=3). Putrescine was detected in three commercially-available dry red wines, the RSD of the determined value was less than 2%, and the recovery were 91.2%～109.3%, indicating that the sensor had good repeatability and high recovery, which could be used for the detection of putrescine in food or biological samples.
- Au nanocluster,
- oxidized lentinan,
- electrochemiluminescence,
- putrescine
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Application of electrochemiluminescence based on oxidized lentinan Au nanocluster composites in the detection of putrescine

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