大黄鱼鱼鳔低聚肽的制备及其延缓皮肤衰老的研究

孙彦庆; 杜芬; 朱宗敏; 冯晓梅; 尚丽丽; 曹婉秀; 刘楚怡

doi:10.12187/2025.01.013

February 2025

Article Contents

SUN Yanqing, DU Fen, ZHU Zongmin, et al. Preparation of swim bladder peptide of Pseudosciaena crocea and its study on delaying skin aging[J]. Journal of Light Industry, 2025, 40(1): 107-119. doi: 10.12187/2025.01.013

Citation: SUN Yanqing, DU Fen, ZHU Zongmin, et al. Preparation of swim bladder peptide of Pseudosciaena crocea and its study on delaying skin aging[J]. Journal of Light Industry, 2025, 40(1): 107-119. doi: 10.12187/2025.01.013 shu

Preparation of swim bladder peptide of Pseudosciaena crocea and its study on delaying skin aging

SUN Yanqing ¹ ,
DU Fen ² ,
ZHU Zongmin ¹ ,
FENG Xiaomei ³ ,
SHANG Lili ¹ ,
CAO Wanxiu ³ ,
LIU Chuyi ^2,3,,

1. Qingdao Daiyoujia Biotechnology Co., Ltd., Qingdao 266001, China;
2. Marine Biomedical Research Institute of Qingdao, Qingdao 266073, China;
3. School of Medicine and Pharmacy, Ocean University of China, Qingdao 266100, China

Corresponding author: LIU Chuyi, liucy@ouc.edu.cn
Received Date: 2024-06-21
Accepted Date: 2024-08-29

Abstract

Using the superoxide anion radical scavenging rate as an indicator, single-factor experiments and response surface methodology were employed to optimize the preparation of peptides from swim bladder of Pseudosciaena crocea (HSBP). The HaCaT cell line, an immortalized human keratinocyte cell line, was employed to establish an oxidative damage model to evaluate the effects of HSBP on collagen and hyaluronic acid secretion, as well as the activities of antioxidant enzymes and β-galactosidase, to assess its oxidative damage repair activity. The 3D recombinant human epidermal barrier injury model was established to study the effects of HSBP on its tissue morphology and the expression of skin barrier related proteins, and to comprehensively evaluate its delaying aging effect on skin. The results showed that the optimal preparation conditions for HSBP were: enzymatic hydrolysis time of 4 hours, 50 ℃, pH value of 7.5, and a bromelain addition of 3400 U/g. Under these conditions, the superoxide anion radical scavenging rate was 65.6%. The molecular weight of HSBP was 480 Da, with no irritation, sensitization, or cytotoxicity. HSBP could significantly promote the secretion of hyaluronic acid, type I and type IV collagen in oxidation-damaged cells, increase the activities of superoxide dismutase and glutathione peroxidase (GSH-Px), decrease the contents of malondialdehyde and β-galactosidase, and significantly increase the expressions of LOR, FLG, TGM1 and AQP3 in barrier damaged models, effectively repair the barrier damaged skin tissue, and significantly increase the number of viable cell layers. Therefore, HSBP could effectively protect skin cells from oxidative damage and repair skin barrier injury, thereby delaying skin aging.
- swim bladder of Pseudosciaena crocea,
- peptide,
- oxidative damage,
- skin barrier damage,
- skin aging
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Preparation of swim bladder peptide of Pseudosciaena crocea and its study on delaying skin aging

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