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水杨酸诱导烟草黄酮类化合物合成基因表达研究

吴晓宗 ,  郭万旺 ,  朱智文 ,  李萌 ,  徐健博 ,  朱若洁 ,  刘志连 ,  耿玉珂

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吴晓宗, 郭万旺, 朱智文, 等. 水杨酸诱导烟草黄酮类化合物合成基因表达研究[J]. 轻工学报, 2025, 40(2): 80-89. doi: 10.12187/2025.02.009
引用本文: 吴晓宗, 郭万旺, 朱智文, 等. 水杨酸诱导烟草黄酮类化合物合成基因表达研究[J]. 轻工学报, 2025, 40(2): 80-89. doi: 10.12187/2025.02.009
shu
WU Xiaozong, GUO Wanwang, ZHU Zhiwen, et al. Studies on flavonoids biosynthesis genes expression induced by salicylic acid in Nicotiana tabacum L.[J]. Journal of Light Industry, 2025, 40(2): 80-89. doi: 10.12187/2025.02.009
Citation: WU Xiaozong, GUO Wanwang, ZHU Zhiwen, et al. Studies on flavonoids biosynthesis genes expression induced by salicylic acid in Nicotiana tabacum L.[J]. Journal of Light Industry, 2025, 40(2): 80-89. doi: 10.12187/2025.02.009
shu

水杨酸诱导烟草黄酮类化合物合成基因表达研究

  • 1. 郑州轻工业大学 烟草科学与工程学院, 河南 郑州 450001;

  • 2. 湖北省烟草公司十堰市公司, 湖北 十堰 442000;

  • 3. 石家庄市藁城区农业科学研究所, 河北 石家庄 052160;

  • 4. 中央民族大学 生命与环境科学学院, 北京 100081

    • 作者简介: 吴晓宗(1981—),男,山西省应县人,郑州轻工业大学副教授,博士,主要研究方向为烟草栽培。E-mail:wuxzong@126.com;
    通讯作者: 耿玉珂,gengyuke0502@163.com

  • 基金项目: 中国烟草总公司重点研发项目(110202202038)

  • 中图分类号: TS41+3;Q786

Studies on flavonoids biosynthesis genes expression induced by salicylic acid in Nicotiana tabacum L.

  • 1. College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;

  • 2. Shiyan Branch of Hubei Tobacco Company, Shiyan 442000, China;

  • 3. Shijiazhuang Gaocheng District Agricultural Science Research Institute, Shijiazhuang 052160, China;

  • 4. College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China

    • Corresponding author: GENG Yuke, gengyuke0502@163.com
  • Received Date: 2024-03-13
    Accepted Date: 2024-05-17

    CLC number: TS41+3;Q786

  • 摘要: 为探索水杨酸(Salicylic Acid,SA)对烟草总黄酮含量及相关基因表达的影响,采用SA溶液对烟草幼苗进行4个时间点(0 h、1 h、4 h和12 h)的灌根处理,检测烟草全株总黄酮含量并进行转录组测序分析,再利用荧光定量PCR对测序分析结果进行验证。结果表明:与0 h处理样品(SA0)相比,1 h处理样品(SA1)的总黄酮含量显著上调,且高于4 h处理样品(SA4)和12 h处理样品(SA12);差异表达基因(DEGs)中,SA诱导SA1、SA4、SA12显著上调的基因重叠数目为1272个,GO功能注释和KEGG代谢通路富集显示,在SA1中显著上调的基因大多编码苯丙素代谢和异黄酮代谢途径相关的催化酶,这些酶能够参与木质素及其衍生物、黄素类、花青素等黄酮类化合物的生物合成;荧光定量PCR中DEGs的相对表达量变化与转录组测序分析结果中相应的表达趋势一致。该研究结果可为挖掘烟草应答SA诱导的黄酮类化合物合成基因、阐明SA与黄酮类化合物生物合成途径之间的调控网络提供依据。
    Abstract: To explore the effects of salicylic acid (SA) on the total flavonoid content of tobacco (Nicotiana tabacumL.) and the expression of flavonoid synthesis-related genes, SA was applied to the one-month tobacco seedlings for 0 h, 1 h, 4 h and 12 h, and the total flavonoid content of the whole tobacco plant was detected and transcriptomic analysis was performed. The results of transcriptome analysis were verified by fluorescence quantitative PCR. The experimental results showed as follows: Compared with 0 h treated sample (SA0), the total flavonoid content of 1 h treated sample (SA1) was significantly up-regulated, and higher than that of 4 h treated sample (SA4) and 12 h treated sample (SA12). Analysis of differentially expressed genes (DEGs) showed that 1272 overlapping genes among SA1, SA4 and SA12 were significantly upregulated by SA treatment, GO functional annotation and KEGG metabolic pathway enrichment dispalyed that most of the genes significantly upregulated in SA1 encoded catalytic enzymes related to phenylpropanoid metabolism and isoflavone metabolism pathway, which could promote the biosynthesis of flavonoids such as lignin and its derivatives, flavins and anthocyanins. Fluorescence quantitative PCR analysis showed that the relative expression changes of differential genes were consistent with the corresponding differential gene expression trends in transcriptome analysis. This study provides clues and basis for seeking the genes involved in SA-induced flavonoids synthesis in tobacco and elucidating the regulatory network between SA and flavonoid biosynthetic pathway.
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  • 通讯作者:  耿玉珂, gengyuke0502@163.com
  • 收稿日期:  2024-03-13
  • 修回日期:  2024-05-17
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吴晓宗, 郭万旺, 朱智文, 等. 水杨酸诱导烟草黄酮类化合物合成基因表达研究[J]. 轻工学报, 2025, 40(2): 80-89. doi: 10.12187/2025.02.009
引用本文: 吴晓宗, 郭万旺, 朱智文, 等. 水杨酸诱导烟草黄酮类化合物合成基因表达研究[J]. 轻工学报, 2025, 40(2): 80-89. doi: 10.12187/2025.02.009
shu
WU Xiaozong, GUO Wanwang, ZHU Zhiwen, et al. Studies on flavonoids biosynthesis genes expression induced by salicylic acid in Nicotiana tabacum L.[J]. Journal of Light Industry, 2025, 40(2): 80-89. doi: 10.12187/2025.02.009
Citation: WU Xiaozong, GUO Wanwang, ZHU Zhiwen, et al. Studies on flavonoids biosynthesis genes expression induced by salicylic acid in Nicotiana tabacum L.[J]. Journal of Light Industry, 2025, 40(2): 80-89. doi: 10.12187/2025.02.009
shu

水杨酸诱导烟草黄酮类化合物合成基因表达研究

    作者简介:吴晓宗(1981—),男,山西省应县人,郑州轻工业大学副教授,博士,主要研究方向为烟草栽培。E-mail:wuxzong@126.com
    通讯作者: 耿玉珂, gengyuke0502@163.com
  • 1. 郑州轻工业大学 烟草科学与工程学院, 河南 郑州 450001;
  • 2. 湖北省烟草公司十堰市公司, 湖北 十堰 442000;
  • 3. 石家庄市藁城区农业科学研究所, 河北 石家庄 052160;
  • 4. 中央民族大学 生命与环境科学学院, 北京 100081
  • 收稿日期: 2024-03-13
  • 录用日期: 2024-05-17
基金项目:  中国烟草总公司重点研发项目(110202202038)

摘要: 为探索水杨酸(Salicylic Acid,SA)对烟草总黄酮含量及相关基因表达的影响,采用SA溶液对烟草幼苗进行4个时间点(0 h、1 h、4 h和12 h)的灌根处理,检测烟草全株总黄酮含量并进行转录组测序分析,再利用荧光定量PCR对测序分析结果进行验证。结果表明:与0 h处理样品(SA0)相比,1 h处理样品(SA1)的总黄酮含量显著上调,且高于4 h处理样品(SA4)和12 h处理样品(SA12);差异表达基因(DEGs)中,SA诱导SA1、SA4、SA12显著上调的基因重叠数目为1272个,GO功能注释和KEGG代谢通路富集显示,在SA1中显著上调的基因大多编码苯丙素代谢和异黄酮代谢途径相关的催化酶,这些酶能够参与木质素及其衍生物、黄素类、花青素等黄酮类化合物的生物合成;荧光定量PCR中DEGs的相对表达量变化与转录组测序分析结果中相应的表达趋势一致。该研究结果可为挖掘烟草应答SA诱导的黄酮类化合物合成基因、阐明SA与黄酮类化合物生物合成途径之间的调控网络提供依据。

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