硒代蛋氨酸对珍珠龙胆石斑鱼肌原纤维蛋白体外消化特性的影响

徐愉聪; 何圣琪; 栾宏伟; 步营; 朱文慧; 励建荣; 李学鹏

doi:10.12187/2025.04.003

August 2025

Article Contents

XU Yucong, HE Shengqi, LUAN Hongwei, et al. Effects of selenomethionine on the in vitro digestibility of myofibrillar proteins in pearl gentian grouper[J]. Journal of Light Industry, 2025, 40(4): 20-29. doi: 10.12187/2025.04.003

Citation: XU Yucong, HE Shengqi, LUAN Hongwei, et al. Effects of selenomethionine on the in vitro digestibility of myofibrillar proteins in pearl gentian grouper[J]. Journal of Light Industry, 2025, 40(4): 20-29. doi: 10.12187/2025.04.003 shu

Effects of selenomethionine on the in vitro digestibility of myofibrillar proteins in pearl gentian grouper

XU Yucong ¹ ,
HE Shengqi ¹ ,
LUAN Hongwei ¹ ,
BU Ying ^1,, ,
ZHU Wenhui ¹ ,
LI Jianrong ^1,2 ,
LI Xuepeng ^1,3

1. College of Food Science and Technology/National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Bohai University, Jinzhou 121013, China;
2. Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China;
3. Institute of Ocean Research, Bohai University, Jinzhou 121013, China

Corresponding author: BU Ying, buying130@126.com
Received Date: 2024-08-17
Accepted Date: 2024-12-19

Abstract

In this study, the effects of selenomethionine on the in vitro digestibility of myofibrillar protein (MP) in pearl gentian grouper were investigated using a static in vitro digestion model to simulate gastricointestinal digestion. The results showed that after simulated gastricointestinal digestion, the selenium content in the high-level selenomethionine group (Y2 group) was 0.130 mg/kg (gastric) and 0.072 mg/kg (intestinal), significantly higher than that in the control group (C group) and low-level selenomethionine group (Y1 group) (P<0.05). Specifically, the carbonyl content (2.02 nmol/mg and 2.59 nmol/mg), dityrosine content (3962 a.u. and 4062 a.u.), thiobarbituric acid(TBA) value (0.31 mg/kg and 0.32 mg/kg), and particle size (365 nm and 357 nm) in the Y2 group were significantly lower than those in the C and Y1 group (P<0.05). Additionally, the Y2 group exhibited higher UV absorption peak intensities and endogenous fluorescence intensities, indicating that high-level selenomethionine delayed MP oxidation and denaturation,thereby better maintaining the structural stability of MP during in vitro digestion.The in vitro digestibility (62.19% and 67.25%) and degree of hydrolysis (0.31 mmol/g and 0.34 mmol/g) in the Y1 group were significantly higher than those in the C and Y2 group (P<0.05). In conclusion, supplementation with selenomethionine not only enhanced the antioxidant capacity of MP but also improved their in vitro digestibility, suggesting that selenomethionine has potential applications in enhancing the nutritional quality and stability of fish proteins.
- pearl gentian grouper,
- selenomethionine,
- myofibrillar protein,
- in vitro digestibility,
- simulated gastricointestinal digestion
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Effects of selenomethionine on the in vitro digestibility of myofibrillar proteins in pearl gentian grouper

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