Structural prediction,cloning expression,and functional verification of NtASAT2 from Nicotiana tabacum L.

Abstract

Keywords:
• Nicotiana tabacum L.,
• acylsugar acyltransferase,
• sucrose ester,
• cloning,
• expression and purification

References

1. [1]

   BAKER I J A,FURLONG D N,GRIESER F,et al.Sugar fatty acid ester surfactants:Base-catalyzed hydrolysis[J].Journal of Surfactants and Detergents,2000,3(1):29-32.

2. [2]

   DAUDÉ D,REMAUD-SIMÉON M,ANDRÉ I.Sucrose analogs:An attractive(bio) source for glycodiversification[J].Natural Product Reports,2012,29(9):945-960.

3. [3]

   FARRÁN A,CAI C,SANDOVAL M,et al.Green solvents in carbohydrate chemistry:From raw materials to fine chemicals[J].Chemical Reviews,2015,115(14):6811-6853.

4. [4]

   马亚茹.糖酯的酶促合成及其功能特性的研究[D].广州:暨南大学,2018.
   

5. [5]

   SCHILMILLER A L,MOGHE G D,FAN P X,et al.Functionally divergent alleles and duplicated loci encoding an acyltransferase contribute to acylsugar metabolite diversity in Solanum trichomes[J].The Plant Cell,2015,27(4):1002-1017.

6. [6]

   SCHILMILLER A L,CHARBONNEAU A L,LAST R L.Identification of a BAHD acetyltransferase that produces protective acyl sugars in tomato trichomes[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(40):16377-16382.

7. [7]

   FAN P X,MILLER A M,SCHILMILLER A L,et al.In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network[J].Proceedings of the National Academy of Sciences of the United States of America,2016,113(2):E239-E248.

8. [8]

   NADAKUDUTI S S,UEBLER J B,LIU X X,et al.Characterization of trichome-expressed BAHD-acyltrans-ferases in Petunia axillaris reveals distinct acylsugar assembly mechanisms within the Solanaceae[J].Plant Physiology,2017,175(1):36-50.

9. [9]

   SLOCOMBE S P,SCHAUVINHOLD I,MCQUINN R P,et al.Transcriptomic and reverse genetic analyses of branched-chain fatty acid and acyl sugar production in Solanum pennellii and Nicotiana benthamiana[J].Plant Physiology,2008,148(4):1830-1846.

10. [10]

    CHANG A X,HU Z Y,CHEN B,et al.Characterization of trichome-specific BAHD acyltransferases involved in acylsugar biosynthesis in Nicotiana tabacum[J].Journal of Experimental Botany,2022,73(12):3913-3928.

11. [11]

    SCHENCK C A,ANTHONY T M,JACOBS M,et al.Natural variation meets synthetic biology:Promiscuous trichome-expressed acyltransferases from Nicotiana[J].Plant Physiology,2022,190(1):146-164.

12. [12]

    ZHENG Z Y,QUALLEY A,FAN B F,et al.An important role of a BAHD acyl transferase-like protein in plant innate immunity[J].Plant Journal,2009,57(6):1040-1053.

13. [13]

    GHOSH B,WESTBROOK T C,JONES A D.Comparative structural profiling of trichome specialized metabolites in tomato(Solanumlycopersicum) and S.habrochaites:Acylsugar profiles revealed by UHPLC/MS and NMR[J].Metabolomics,2014,10(3):496-507.

14. [14]

    SEVERSON R F,ARRENDALE R F,CHORTYK O T,et al.Isolation and characterization of the sucrose esters of the cuticular waxes of green tobacco leaf[J].Journal of Agricultural and Food Chemistry,1985,33(5):870-875.

15. [15]

    黄申,闫茗熠,陈梦月,等.基于转录组测序和RT-qPCR技术的烟草糖酯合成基因挖掘[J].轻工学报,2023,38(6):78-84.
    

16. [16]

    周烨,刘哲益,王方军.结构蛋白质组学研究进展[J].色谱,2019,37(8):788-797.
    

17. [17]

    熊强,丁立新,姜晓燕,等.X射线测定蛋白质结构的技术进展与研究现状[J].癌变·畸变·突变,2019,31(1):82-85.
    

18. [18]

    韩丽,王丽娇,肖成志,等.烟草NtASAT1基因的原核表达、纯化及功能验证[J].中国生物工程杂志,2023,43(4):30-40.
    

19. [19]

    DHAKAL S,SAPKOTA K,HUANG F Q,et al.Cloning,expression and purification of the low-complexity region of RanBP9 protein[J].Protein Expression and Purification,2020,172:105630.

20. [20]

    PUIG O,CASPARY F,RIGAUT G,et al.The tandem affinity purification(TAP) method:A general procedure of protein complex purification[J].Methods,2001,24(3):218-229.

21. [21]

    罗莉,李坤,王保成,等.包涵体变复性技术研究进展[J].中国医药生物技术,2012,7(4):290-293.
    

22. [22]

    KURIATA A,IGLESIAS V,PUJOLS J,et al.Aggrescan3D(A3D)2.0:Prediction and engineering of protein solubility[J].Nucleic Acids Research,2019,47(W1):W300-W307.

23. [23]

    苗朝悦,杜乐,王佳琦,等.重组蛋白质在大肠杆菌体系中的可溶性表达策略[J].中国生物工程杂志,2023,43(9):33-45.
    

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