果胶酶粗酶液与漆酶粗酶液复配酶解膨胀梗丝的工艺优化

齐汉如; 欧阳少丰; 李玉; 杨雪鹏; 赵建国

doi:10.12187/2025.04.011

August 2025

Article Contents

QI Hanru, OUYANG Shaofeng, LI Yu, et al. Optimization of enzymatic hydrolysis process for expanded tobacco cut stem using combined crude enzymes of pectinase and laccase[J]. Journal of Light Industry, 2025, 40(4): 96-107. doi: 10.12187/2025.04.011

Citation: QI Hanru, OUYANG Shaofeng, LI Yu, et al. Optimization of enzymatic hydrolysis process for expanded tobacco cut stem using combined crude enzymes of pectinase and laccase[J]. Journal of Light Industry, 2025, 40(4): 96-107. doi: 10.12187/2025.04.011 shu

Optimization of enzymatic hydrolysis process for expanded tobacco cut stem using combined crude enzymes of pectinase and laccase

1. College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
2. Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Corresponding author: ZHAO Jianguo, 2016020@zzuli.edu.cn
Received Date: 2024-11-01
Accepted Date: 2024-12-24

Abstract

Pectinase-producing strain TS-1 and laccase-producing strain TS-2 were isolated from tobacco field soil and decayed tobacco stems. Morphological observation and molecular biological identification confirmed their taxonomic status as Aspergillus niger and Phanerodontia chrysosporium, respectively. Enzyme production conditions were optimized via single-factor experiments: for TS-1 pectinase production, the optimal parameters were tobacco stem powder concentration 50 g/L, fermentation temperature 30 ℃, and pH value 6.0; for TS-2 laccase production, the optimal conditions were tobacco stem powder concentration 50 g/L, (NH₄)₂SO₄ concentration 0.6 g/L, and fermentation temperature 30 ℃. The hydrolysis conditions for pectin in expanded tobacco cut stems were optimized as crude pectinase dosage 240 U/g, hydrolysis time 4.0 h, liquid-to-solid ratio 1∶2, at 30 ℃; for lignin hydrolysis, the optimal conditions were crude laccase dosage 2.0 U/g, hydrolysis time 3.5 h, liquid-to-solid ratio 1∶2, at 30 ℃. The co-application of crude enzymes achieved optimal hydrolysis at pectinase dosage 170 U/g, laccase dosage 1.5 U/g, and hydrolysis time 3.0 h. Post-hydrolysis, the sensory evaluation score of expanded cut stems increased by 8.2 points compared to untreated samples, accompanied by a significant elevation in aroma constituent content.
- tobacco stem,
- pectinase,
- laccase,
- enzymatic hydrolysis process,
- expanded tobacco cut stem,
- sensory quality,
- aroma constituent
References
1. [1]
  FAN X,ZI W H,AO J C,et al.Analysis and application evaluation of the flavour-precursor and volatile-aroma-component differences between waste tobacco stems[J].Heliyon,2022,8(9):e10658.
2. [2]
  南哲洙,仝智强,姚思愚,等.超声洗梗工艺对烟梗理化特性及卷烟感官质量的影响[J].烟草科技,2024,57(4):78-84
  ,90.
3. [3]
  孔浩辉,李秀丽,黄翼飞,等.氢氧化钠/尿素法和硫酸法测定烟草木质素的对比分析[J].烟草科技,2015,48(1):66-71.
4. [4]
  陈志浩,丁丽,李栋,等.溶剂热法降低烟梗中木质素含量及提高发烟剂负载量的初步研究[J].中国烟草学报,2022,28(2):27-32.
5. [5]
  余其昌,黄菲,易聪华,等.甘油碱法脱除烟梗中的木质素[J].中国烟草学报,2018,24(4):1-6.
6. [6]
  李强,吴晓青,张新建.微生物降解秸秆木质素的研究进展[J].微生物学报,2023,63(11):4118-4132.
7. [7]
  苏玉龙.高效降解烟秆菌株筛选及其特性研究[D].北京:中国农业科学院,2016.
8. [8]
  王文婷,宋凯,杨晨,等.基于宏基因组学的霍氏肠杆菌发酵解析及其烟叶品质提升机制研究[J].轻工学报,2023,38(1):79-89.
9. [9]
  李林玮,毛亚浩,余君,等.巨大芽孢杆菌对雪茄烟叶品质及细菌群落结构的影响[J].湖南农业科学,2023(11):70-78.
10. [10]
  吴新惠,杜金杰,刘晓纯,等.六堡茶真菌固态发酵优势单菌的筛选[J].食品科学,2024,45(6):64-71.
11. [11]
  全铭沁,董惠忠,沙云菲,等.黑曲霉发酵液改善上部烟叶风味品质的研究[J].中国酿造,2019,38(3):170-176.
12. [12]
  宋丽丽,张永良,张志平,等.白腐菌液体发酵降解烟梗木质素的研究[J].轻工学报,2019,34(1):36-42.
13. [13]
  ZHAO J G,OUYANG S F,QI H R,et al.Metabolomics and transcriptomics uncover the pectin hydrolysis during tobacco stem fermentation by Aspergillus niger[J].Journal of Cleaner Production,2024,442:141005.
14. [14]
  陈建军,刘梁涛,曹香林.高效木质素降解菌的筛选及产漆酶条件的研究[J].甘肃农业大学学报,2018,53(4):130-136.
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
  罗雯,徐珂盼,王怡,等.果胶酶生产菌株的筛选、发酵优化及其酶学性质研究[J].食品科技,2022,47(4):6-13.
20. [20]
  陈勇强.黑曲霉液态发酵橘子皮产果胶酶工艺条件优化[J].现代农业科技,2021(24):194-196,204.
21. [21]
  郑耀通,李文燕.黄孢原毛平革菌抗营养阻遏产漆酶同工酶动态分析[J].微生物学杂志,2020,40(6):13-20.
22. [22]
  张晓瑞,刘晓晖,付博,等.烟草中淀粉降解菌的筛选、鉴定及发酵工艺优化[J].食品与机械,2021,37(2):34-41.
23. [23]
  ZHU B B,AN H Y,LI L,et al.Characterization of flavor profiles of cigar tobacco leaves grown in China via headspace-gas chromatography-ion mobility spectrometry coupled with multivariate analysis and sensory evaluation[J].ACS Omega,2024,9(14):15996-16005.
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沈阳化工大学材料科学与工程学院沈阳 110142

Optimization of enzymatic hydrolysis process for expanded tobacco cut stem using combined crude enzymes of pectinase and laccase

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