JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 40 Issue 5
October 2025
Article Contents
HOU Qiangchuan, ZHANG Tian, WANG Junlin, et al. Study on the flavor quality and bacterial diversity of high-temperature Daqu with different colors produced by Longzhongdui winery[J]. Journal of Light Industry, 2025, 40(5): 11-19. doi: 10.12187/2025.05.002
Citation: HOU Qiangchuan, ZHANG Tian, WANG Junlin, et al. Study on the flavor quality and bacterial diversity of high-temperature Daqu with different colors produced by Longzhongdui winery[J]. Journal of Light Industry, 2025, 40(5): 11-19. doi: 10.12187/2025.05.002 shu

Study on the flavor quality and bacterial diversity of high-temperature Daqu with different colors produced by Longzhongdui winery

  • Corresponding author: GUO Zhuang, guozhuang1984@163.com
  • Received Date: 2024-11-08
    Accepted Date: 2025-03-11
  • Electronic sensing technology and MiSeq high-throughput sequencing technology were used to study the flavor quality and bacterial diversity of black high-temperature Daqu (black-HTD), white high-temperature Daqu (white-HTD), and yellow high-temperature Daqu (yellow-HTD) produced by Longzhongdui winery. The results showed that in terms of flavor, white-HTD had relatively high contents of aromatic compounds, alkanes, and lipids, black-HTD had relatively high contents of sulfur compounds, alcohols, aldehydes, and ketones, and the contents of aroma components in yellow-HTD were between those of black-HTD and white-HTD. In terms of taste, black-HTD had relatively unique taste characteristics and was significantly better than white-HTD and yellow-HTD in terms of acidity, bitterness, astringency, richness (umami aftertaste), aftertaste A (astringent aftertaste), and aftertaste B (bitter aftertaste) (P< 0.05). In terms of microbial composition, there were no significant differences in bacterial diversity. However, the bacterial richness in white-HTD was significantly higher than that in black-HTD (P< 0.05). The dominant bacterial genera in the three types of high-temperature Daqu mainly included Saccharopolyspora (28.95%), Thermoactinomyces (21.07%), Kroppenstedtia (10.06%), and Bacillus (8.94%). There was a significant correlation between the microbial community structure and flavor quality of the three types of high-temperature Daqu. Specifically, Kroppenstedtia showed a significant positive correlation with the response values of W1C (sensitive to aromatic hydrocarbon compounds) and W3C (sensitive to ammonia and aromatic components) (P< 0.05). Thermoactinomyces and Weissella showed significant positive correlations with umami indicators but significant negative correlations with sourness, astringency, aftertaste A, and aftertaste B (P< 0.05).
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    1. [1]

      WANG B W,WU Q,XU Y,et al.Specific volumetric weight-driven shift in microbiota compositions with saccharifying activity change in starter for Chinese Baijiu fermentation[J].Frontiers in Microbiology,2018,9:2349.

    2. [2]

      ZHENG X W,TABRIZI M R,ROBERT NOUT M J,et al.Daqu-A traditional Chinese liquor fermentation starter[J].Journal of the Institute of Brewing,2011,117(1):82-90.

    3. [3]

      YANG L,FAN W L,XU Y.Qu-omics elucidates the formation and spatio-temporal differentiation mechanism underlying the microecology of high temperature Daqu[J].Food Chemistry,2024,438(4):137988.

    4. [4]

      GUAN T W,YANG H,OU M Y,et al.Storage period affecting dynamic succession of microbiota and quality changes of strong-flavor Baijiu Daqu[J].LWT-Food Science and Technology,2021,139:110544.

    5. [5]

      CAI W C,WANG Y R,NI H,et al.Diversity of microbiota,microbial functions,and flavor in different types of low-temperature Daqu[J].Food Research International,2021,150:110734.

    6. [6]

      XIAO C,LU Z M,ZHANG X J,et al.Bio-heat is a key environmental driver shaping the microbial community of medium-temperature Daqu[J].Applied and Environmental Microbiology,2017,83(23):e01550-17.

    7. [7]

      SHI W,CHAI L J,FANG G Y,et al.Spatial heterogeneity of the microbiome and metabolome profiles of high-temperature Daqu in the same workshop[J].Food Research International,2022,156:111298.

    8. [8]

      ZHU Q,CHEN L Q,PU X X,et al.The differences in the composition of Maillard components between three kinds of sauce-flavor Daqu[J].Fermentation,2023,9(9):860.

    9. [9]

      ZHU Q,CHEN L Q,PENG Z,et al.Analysis of environmental driving factors on Core Functional Community during Daqu fermentation[J].Food Research International,2022,157:111286.

    10. [10]

      CAI W C,WANG Y R,LIU Z J,et al.Depth-depended quality comparison of light-flavor fermented grains from two fermentation rounds[J].Food Research International,2022,159:111587.

    11. [11]

      毛凤娇,黄钧,周荣清,等.高效定向驯化人工窖泥功能菌群及机制的研究[J].食品与发酵工业,2024,50(8):211-218.

    12. [12]

      WANG Y R,CAI W C,WANG W P,et al.Analysis of microbial diversity and functional differences in different types of high-temperature Daqu[J].Food Science & Nutrition,2020,9(2):1003-1016.

    13. [13]

      侯强川,王玉荣,王文平,等.茅台和尧治河高温大曲细菌群落结构差异及功能预测[J].食品与发酵工业,2022,48(1):36-44.

    14. [14]

      GUO Z,XIANG F S,HOU Q C,et al.Analysis of bacterial community structure and taste quality of different colored high-temperature Daqu in the Xiangyang region,China[J].LWT-Food Science and Technology,2023,188:115411.

    15. [15]

      CAI W C,XUE Y A,WANG Y R,et al.The fungal communities and flavor profiles in different types of high-temperature Daqu as revealed by high-throughput sequencing and electronic senses[J].Frontiers in Microbiology,2021,12:784651.

    16. [16]

      KARAKAYA D,ULUCAN O,TURKAN M.Electronic nose and its applications:A survey[J].International Journal of Automation and Computing,2020,17(2):179-209.

    17. [17]

      ROSS C F.Considerations of the use of the electronic tongue in sensory science[J].Current Opinion in Food Science,2021,40:87-93.

    18. [18]

      HUANG M Z,LI T T,HARDIE W J,et al.Comparative characterization and sensory significance of volatile compounds in Rosa roxburghii Tratt fruit from five geographic locations in Guizhou,China[J].Flavour and Fragrance Journal,2022,37(3):163-180.

    19. [19]

      孙文佳,方毅斐,汪廷彩,等.基于电子舌技术的豉香型白酒分类识别研究[J].中国酿造,2020,39(9):182-185.

    20. [20]

      郭壮,葛东颖,尚雪娇,等.退化和正常窖泥微生物多样性的比较分析[J].食品工业科技,2018,39(22):93-98.

    21. [21]

      WEI Z G,ZHANG X D,CAO M,et al.Comparison of methods for picking the operational taxonomic units from amplicon sequences[J].Frontiers in Microbiology,2021,12:644012.

    22. [22]

      POLLOCK J,GLENDINNING L,WISEDCHANWET T,et al.The madness of microbiome:Attempting to find consensus “best practice”for 16S microbiome studies[J].Applied and Environmental Microbiology,2018,84(7):e02627-17.

    23. [23]

      BRANDT M I,TROUCHE B,QUINTRIC L,et al.Bioinformatic pipelines combining denoising and clustering tools allow for more comprehensive prokaryotic and eukaryotic metabarcoding[J].Molecular Ecology Resources,2021,21(6):1904-1921.

    24. [24]

      LUO S,ZHANG Q L,YANG F,et al.Analysis of the formation of sauce-flavored Daqu using non-targeted metabolomics[J].Frontiers in Microbiology,2022,13:857966.

    25. [25]

      VAN BOEKEL M A J S.Formation of flavour compounds in the Maillard reaction[J].Biotechnology Advances,2006,24(2):230-233.

    26. [26]

      XI X,YANG F,CHEN L,et al.Dynamic changes of bacteria communities in Moutai-flavour Daqu during storage analysed by next generation sequencing[J].Letters in Applied Microbiology,2022,75(6):1486-1496.

    27. [27]

      XIA Y,ZHU M,DU Y K,et al.Metaproteomics reveals protein composition of multiple saccharifying enzymes in Nongxiangxing Daqu and Jiangxiangxing Daqu under different thermophilic temperatures[J].International Journal of Food Science & Technology,2022,57(8):5102.

    28. [28]

      QUAN S K,WANG Y,RAN M F,et al.Contrasting the microbial community and non-volatile metabolites involved in ester synthesis between Qing-flavor Daqu and Nong-flavor Daqu[J].Journal of Bioscience and Bioengineering,2023,136(3):213-222.

    29. [29]

      CHEN G M,YUAN Y J,TANG S W,et al.Comparative analysis of microbial communities and volatile flavor components in the brewing of Hongqu rice wines fermented with different starters[J].Current Research in Food Science,2023,7:100628.

    30. [30]

      DOYLE C J,GLEESON D,O'TOOLE P W,et al.Impacts of seasonal housing and teat preparation on raw milk microbiota:A high-throughput sequencing study[J].Applied and Environmental Microbiology,2016,83(2):e02694-16.

    31. [31]

      YUE S J,HUANG P,LI S,et al.Enhanced production of 2-hydroxyphenazine from glycerol by a two-stage fermentation strategy in Pseudomonas chlororaphis GP72AN[J].Journal of Agricultural and Food Chemistry,2020,68(2):561-566.

    32. [32]

      ZHUANSUN W W,XU J,LI Z Q,et al.Dynamic changes in the microbial community,flavour components in Jiupei of a novel Maotai-Luzhou-flavoured liquor under various daqu blending modes and their correlation analysis[J].LWT-Food Science and Technology,2022,172:114167.

    33. [33]

      WANG Y,QUAN S K,XIA Y,et al.Exploring the regulated effects of solid-state fortified Jiuqu and liquid-state fortified agent on Chinese Baijiu brewing[J].Food Research International,2024,179:114024.

    34. [34]

      ZENG Y,WANG Y,CHEN Q,et al.Dynamics of microbial community structure and enzyme activities during the solid-state fermentation of Forgood Daqu:A starter of Chinese strong flavour Baijiu[J].Archives of Microbiology,2022,204(9):577.

    35. [35]

      ZHU C T,CHENG Y X,SHI Q L,et al.Metagenomic analyses reveal microbial communities and functional differences between Daqu from seven provinces[J].Food Research International,2023,172:113076.

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