噬菌体及其衍生物抗食源性致病菌生物被膜的研究进展

孙新城; 杜月霞; 周军; 杜婷婷; 秦金梦; 张思威; 张晓根

doi:10.12187/2025.05.005

噬菌体及其衍生物抗食源性致病菌生物被膜的研究进展

1. 郑州轻工业大学食品与生物工程学院, 河南郑州 450001;
2. 食品生产与安全河南省协同创新中心, 河南郑州 450001;
3. 中原食品实验室郑州轻工业大学, 河南漯河 462300;
4. 冷链食品加工与安全控制教育部重点实验室(培育), 河南郑州 450001

作者简介: 孙新城(1976—),男,山东省巨野县人,郑州轻工业大学教授,博士,主要研究方向为食品微生物。E-mail:biosxc@126.com;

基金项目: 河南省科技攻关项目(242102111045,222102520041,222103810020)
中图分类号: TS201.3;Q93

Research progress of bacteriophages and their derivatives against foodborne pathogens biofilms

1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
2. Collaborative Innovation Center of Food Production and Safety, Henan Province, Zhengzhou 450001, China;
3. Zhengzhou University of Light Industry, Food Laboratory of Zhongyuan, Luohe 462300, China;
4. Key Laboratory of Cold Chain Food Processing and Safety Control, Ministry of Education(Cultivation), Zhengzhou 450001, China
Received Date: 2024-11-04
Accepted Date: 2025-03-12

CLC number: TS201.3;Q93

摘要: 生物被膜(Biofilm,BF)是食源性致病菌在食品加工过程中的主要存在形式,其结构复杂,常规消杀手段难以根除,其防控策略正逐步成为食品领域的研究重点。基于食源性致病菌防控面临的严峻形势及噬菌体作为生物防治剂的潜力,对生物被膜的形成过程及主要调控机制,以及噬菌体及其衍生物、噬菌体与抗生素联用抗食源性致病菌生物被膜的研究进展进行综述。生物被膜的形成过程大致包括可逆粘附阶段、不可逆粘附阶段、早期形成阶段、成熟阶段和分散阶段,群体感应是调控生物被膜形成的主要因素;噬菌体可特异性地靶向食源性致病菌,其衍生物裂解酶和穿孔素能降解细菌的肽聚糖,使细菌细胞裂解并释放子代噬菌体;噬菌体与抗生素联用可产生协同效应,提高食源性致病菌生物被膜的防控效果。未来研究将结合大健康(One Health)理念,从医学、食品、环境等领域解析生物被膜在“人-动物-环境”中的交叉传播机制,优化噬菌体-抗生素联用条件,提升多策略协同防控效果,以更好地保障食品质量与安全。
- 食源性致病菌 /
- 生物被膜 /
- 噬菌体 /
- 生物防治剂 /
- 抗生素
Abstract: Biofilm (BF) is the predominant form of foodborne pathogenic bacteria during food processing. It has a complex structure, making it difficult for conventional disinfection and sterilization methods to eradicate it. Prevention and control strategies for biofilms are gradually becoming a research focus in the food field. Based on the severe situation in the prevention and control of foodborne pathogenic bacteria and the potential of bacteriophages as biological control agents, this review summarizes the formation process and main regulatory mechanisms of biofilms, as well as the research progress on bacteriophages and their derivatives, and the combination of bacteriophages and antibiotics in targeting biofilms of foodborne pathogenic bacteria. The formation process of biofilm generally includes the reversible adhesion stage, irreversible adhesion stage, early formation stage, maturation stage, and dispersion stage. Quorum sensing is the main factor that regulates biofilm formation. Bacteriophages can specifically target foodborne pathogenic bacteria. Their derivatives, lysin and holin, can degrade the peptidoglycan of bacteria, leading to bacterial cell lysis and the release of progeny bacteriophages. The combination of bacteriophages and antibiotics can produce a synergistic effect and improve the effect of prevention and control of biofilms formed by foodborne pathogenic bacteria. Future research will integrate the One Health concept, investigate the cross-transmission mechanism of biofilms in the “human-animal-environment” system from the fields of medicine, food, and environment, optimize the combination conditions of bacteriophages and antibiotics, and enhance the effect of multi-strategy synergistic prevention and control to better ensure food quality and safety.
- foodborne pathogenic bacteria /
- biofilm /
- phage /
- biological control agent /
- antibiotic
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沈阳化工大学材料科学与工程学院沈阳 110142

噬菌体及其衍生物抗食源性致病菌生物被膜的研究进展

作者简介: 孙新城(1976—),男,山东省巨野县人,郑州轻工业大学教授,博士,主要研究方向为食品微生物。E-mail:biosxc@126.com;

Research progress of bacteriophages and their derivatives against foodborne pathogens biofilms

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通讯作者: 陈斌, bchen63@163.com

噬菌体及其衍生物抗食源性致病菌生物被膜的研究进展

作者简介:孙新城(1976—),男,山东省巨野县人,郑州轻工业大学教授,博士,主要研究方向为食品微生物。E-mail:biosxc@126.com

English Abstract

Research progress of bacteriophages and their derivatives against foodborne pathogens biofilms

目录

噬菌体及其衍生物抗食源性致病菌生物被膜的研究进展

作者简介: 孙新城(1976—),男,山东省巨野县人,郑州轻工业大学教授,博士,主要研究方向为食品微生物。E-mail:biosxc@126.com;

Research progress of bacteriophages and their derivatives against foodborne pathogens biofilms

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通讯作者: 陈斌, bchen63@163.com

噬菌体及其衍生物抗食源性致病菌生物被膜的研究进展

作者简介:孙新城(1976—),男,山东省巨野县人,郑州轻工业大学教授,博士,主要研究方向为食品微生物。E-mail:biosxc@126.com

English Abstract

Research progress of bacteriophages and their derivatives against foodborne pathogens biofilms

目录