1株沙门氏菌噬菌体的分离鉴定及其抑菌效果研究

周军; 黄俊清; 杜婷婷; 秦金梦; 张思威; 孙世彪; 孙新城

doi:10.12187/2026.01.006

1株沙门氏菌噬菌体的分离鉴定及其抑菌效果研究

1. 郑州轻工业大学材料与化学工程学院, 河南郑州 450001;
2. 郑州轻工业大学食品与生物工程学院, 河南郑州 450001;
3. 中原食品实验室郑州轻工业大学, 河南漯河 462300;
4. 食品生产与安全河南省协同创新中心, 河南郑州 450001;
5. 河南省冷链食品质量安全控制重点实验室, 河南郑州 450001

作者简介: 周军(1976—),男,湖南省邵阳市人,郑州轻工业大学讲师,博士,主要研究方向为环境工程。E-mail:2005006@zzuli.edu.cn;

通讯作者: 孙新城,biosxc@126.com

基金项目: 河南省科技攻关项目(242102111045)
中图分类号: TS201.3;Q93

Isolation, identification and antibacterial effect of a Salmonella phage

1. College of Materials and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
2. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
3. Food Laboratory of Zhongyuan, Zhengzhou University of Light Industry, Luohe 462300, China;
4. Henan Collaborative Innovation Centre of Food Production Safety, Zhengzhou 450001, China;
5. Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou 450001, China

Corresponding author: SUN Xincheng, biosxc@126.com

Received Date: 2025-03-26
Accepted Date: 2025-09-03

CLC number: TS201.3;Q93

摘要: 【目的】探索噬菌体抑制食品中沙门氏菌的效果。【方法】采用双层琼脂平板法,以鼠伤寒沙门氏菌(Salmonella typhimurium)为宿主菌,从采集自郑州某污水厂的污水样品中分离纯化噬菌体,研究其生物学特性、基因组特征及对人工污染牛奶和蛋清中鼠伤寒沙门氏菌的抑菌效果。【结果】分离纯化获得的噬菌体ST-1的最佳感染复数(MOI)为0.1,最高效价为10.45 lg PFU/mL,潜伏期为20 min,裂解期为70 min,裂解量约为106.5 PFU/cell;宿主谱较窄,仅能裂解鼠伤寒沙门氏菌和肺炎链球菌;稳定性较好,在温度30~60 ℃和pH值5~10范围内均能保持活性,同时对氯仿不敏感;属于尾噬菌体目,基因组大小为84 672 bp,GC含量为38.82%,含有142个开放阅读框(ORF),不含抗性基因和毒力基因,能显著抑制人工污染牛奶和蛋清中鼠伤寒沙门氏菌的生长。【结论】噬菌体ST-1的裂解效率较高,热稳定性、酸碱耐受性、安全性和防控效果均较好,可为靶向沙门氏菌生物防治剂的研制提供参考。
- 沙门氏菌 /
- 噬菌体 /
- 生物学特性 /
- 基因组特征 /
- 抑菌效果
Abstract: 【Objective】 This study aims to explore the antibacterial effect of phages on Salmonella in food. 【Methods】 Phages were isolated and purified from wastewater samples collected from a wastewater treatment plant in Zhengzhou, with Salmonella typhimurium as the host bacterium, by the double-layer agar plate method. Their biological characteristics, genomic features, and antibacterial effects on S.typhimurium in artificially contaminated milk and egg white were investigated. 【Results】 The optimal multiplicity of infection (MOI) of the isolated and purified phage ST-1 was 0.1, with a maximum titer of 10.45 lg PFU/mL. Its latent period was 20 min, the lysis period was 70 min, and the burst size was approximately 106.5 PFU/cell. It had a narrow host range, being able to lyse only S.typhimurium and Streptococcus pneumoniae. It exhibited good stability, maintaining activity within a temperature range of 30~60 ℃ and a pH range of 5~10, while also being insensitive to chloroform. Phage ST-1 belongs to the order Caudovirales. Its genome size is 84 672 bp, with a GC content of 38.82%, and it contains 142 open reading frames (ORFs). It does not carry resistance genes or virulence genes and can significantly inhibit the growth of S.typhimurium in artificially contaminated milk and egg white. 【Conclusion】 Phage ST-1 has high lysis efficiency, good thermal stability, acid-base tolerance, safety, and control effects, which can provide a reference for the development of targeted biocontrol agents against Salmonella.
- Salmonella /
- phage /
- biological characteristic /
- genomic feature /
- antibacterial effect
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1株沙门氏菌噬菌体的分离鉴定及其抑菌效果研究

作者简介: 周军(1976—),男,湖南省邵阳市人,郑州轻工业大学讲师,博士,主要研究方向为环境工程。E-mail:2005006@zzuli.edu.cn;

通讯作者: 孙新城,biosxc@126.com

Isolation, identification and antibacterial effect of a Salmonella phage

Corresponding author: SUN Xincheng, biosxc@126.com

计量

通讯作者: 陈斌, bchen63@163.com

1株沙门氏菌噬菌体的分离鉴定及其抑菌效果研究

作者简介:周军(1976—),男,湖南省邵阳市人,郑州轻工业大学讲师,博士,主要研究方向为环境工程。E-mail:2005006@zzuli.edu.cn

通讯作者: 孙新城, biosxc@126.com

English Abstract

Isolation, identification and antibacterial effect of a Salmonella phage

Corresponding author: SUN Xincheng, biosxc@126.com

目录

1株沙门氏菌噬菌体的分离鉴定及其抑菌效果研究

作者简介: 周军(1976—),男,湖南省邵阳市人,郑州轻工业大学讲师,博士,主要研究方向为环境工程。E-mail:2005006@zzuli.edu.cn;

通讯作者: 孙新城,biosxc@126.com

Isolation, identification and antibacterial effect of a Salmonella phage

Corresponding author: SUN Xincheng, biosxc@126.com

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