固相萃取-超高效液相色谱-多级质谱联用法测定烟叶中5种类胡萝卜素的含量

李山; 张瑾洁; 王志才; 刘光伟; 李星亮; 白冰; 毛多斌; 贾春晓

doi:10.12187/2026.02.011

April 2026

Article Contents

LI Shan, ZHANG Jinjie, WANG Zhicai, et al. Simultaneous determination of five carotenoids in tobacco leaves by solid-phase extraction combined with ultra-high performance liquid chromatography- multistage mass spectrometry[J]. Journal of Light Industry, 2026, 41(2): 116-125. doi: 10.12187/2026.02.011

Citation: LI Shan, ZHANG Jinjie, WANG Zhicai, et al. Simultaneous determination of five carotenoids in tobacco leaves by solid-phase extraction combined with ultra-high performance liquid chromatography- multistage mass spectrometry[J]. Journal of Light Industry, 2026, 41(2): 116-125. doi: 10.12187/2026.02.011 shu

Simultaneous determination of five carotenoids in tobacco leaves by solid-phase extraction combined with ultra-high performance liquid chromatography- multistage mass spectrometry

1. Technology R&D Center, Gansu Tobacco Industrial Co., Ltd., Lanzhou 730050, China;
2. No. 1 Junior High School of Pingyu, Zhumadian 463400, China;
3. College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Corresponding author: JIA Chunxiao, jiachx@zzuli.edu.cn
Received Date: 2024-12-31
Accepted Date: 2025-03-10

Abstract

【Objective】 To develop a qualitative and quantitative analytical method for simultaneously determining five carotenoids in tobacco leaves. 【Methods】 Tobacco leaves were extracted and purified by ultrasonic extraction combined with solid-phase extraction, and the carotenoids were quantitatively analyzed by ultra-high performance liquid chromatography-multistage mass spectrometry (UHPLC-MSⁿ). 【Results】 The optimal extraction conditions were as follows: acetone was used as the ultrasonic extraction solvent, with a solid-to-liquid ratio of 1∶40, ultrasonic power of 120 W, and ultrasonic time of 50 min; while the optimal purification conditions were: HF Bond Elut C18 column, acetonitrile as the leaching solution, and dichloromethane as the eluent. Under the selected reaction monitoring (SRM) mode, the standard curves of the five carotenoids showed a good linear relationship with correlation coefficients (r) all greater than 0.999 5. The limits of detection (LOD) of the method were 66.00~224.00 μg/kg, the limits of quantification (LOQ) were 18.20~67.20 μg/kg, the average recoveries were 88.3%~93.3%, and the relative standard deviations (RSDs) were all less than 5%. 【Conclusion】 This method is simple, highly sensitive and precise, which is suitable for the qualitative and quantitative analysis of the main carotenoids in tobacco leaves. It provides a reference for the research on the metabolic mechanisms of tobacco aroma precursors and the targeted improvement of tobacco leaf flavor quality.
- tobacco leaves,
- solid-phase extraction,
- UHPLC-MSⁿ,
- carotenoids
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Simultaneous determination of five carotenoids in tobacco leaves by solid-phase extraction combined with ultra-high performance liquid chromatography- multistage mass spectrometry

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