烟草糖苷降解菌筛选及糖苷酶挖掘

冯颖杰; 齐文渊; 刘文召; 张婷婷; 杨金初; 杨宗灿; 朱丽; 黄申

doi:10.12187/2026.03.011

烟草糖苷降解菌筛选及糖苷酶挖掘

冯颖杰 ¹ ,
齐文渊 ² ,
刘文召 ¹ ,
张婷婷 ¹ ,
杨金初 ¹ ,
杨宗灿 ¹ ,
朱丽 ² ,
黄申 ^{2

,

,}

1. 河南中烟工业有限责任公司技术中心, 河南郑州 450000;
2. 郑州轻工业大学烟草科学与工程学院, 河南郑州 450001

作者简介: 冯颖杰(1990—),男,安徽省萧县人,河南中烟工业有限责任公司高级工程师,主要研究方向为烟草生物技术。E-mail:fyjwin123@163.com;

通讯作者: 黄申,huangshen@zzuli.edu.cn

基金项目: 中国烟草总公司重点科技项目(110202202006)
河南中烟工业有限责任公司科技项目(AW2022017)
中图分类号: TS41.4

Screening of tobacco glycoside-degrading bacteria and mining of glycosidases

1. Technology Center, China Tobacco He'nan Industrial Co., Ltd., Zhengzhou 450000, China;
2. College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Corresponding author: HUANG Shen, huangshen@zzuli.edu.cn

Received Date: 2024-09-19
Accepted Date: 2025-03-21

CLC number: TS41.4

摘要: 【目的】开发降解烟草糖苷类化合物并生成香味成分的生物酶。【方法】以3-氧代-α-紫罗兰醇-β-D-吡喃葡萄糖苷为唯一碳源筛选降解烟草糖苷的菌株,对该菌株进行基因组分析,分离纯化降解菌糖苷酶,考查糖苷酶的酶学特性,鉴定并挖掘烟草糖苷酶。【结果】菌株HZW-15可降解3-氧代-α-紫罗兰醇-β-D-吡喃葡萄糖苷,其粗酶处理烟草粗糖苷后,3-氧代-α-紫罗兰醇、苯甲醇、苯乙醇等香味成分显著增加;酶液处理再造烟叶浓缩液后甜感突出、余味干净、香气质改善;当酶解温度40 ℃,转速150 r/min,pH值7,钾离子浓度10 mmol/L时,3-氧代-α-紫罗兰醇含量增加了2.3倍;HZW-15基因组共有5 554 921个碱基,编码76个糖苷水解酶基因。【结论】利用质谱鉴定纯化后的酶组分,推测6-磷酸-β-葡萄糖苷酶、β-葡萄糖糖苷酶和1,4-α-葡聚糖分支酶为功能酶,可为烟用糖苷酶的开发提供参考。
- 烟草糖苷 /
- 降解菌 /
- 糖苷酶 /
- 酶学特性
Abstract: 【Objective】 To develop enzymes that degrade tobacco glycosides and generate aroma components. 【Methods】 A strain capable of degrading 3-oxo-α-ionol-β-D-glucopyranoside was screened using 3-oxo-α-ionol-β-D-glucopyranoside as the sole carbon source. The genome of this strain was analyzed, and the glycosidase from it was isolated and purified. The enzymatic properties of the glycosidase were investigated, and tobacco glycosidases were identified and mined. 【Results】 Strain HZW-15 was capable of degrading 3-oxo-α-ionol-β-D-glucopyranoside. After treating crude tobacco glycosides with its crude enzyme, the contents of aroma components such as 3-oxo-α-ionol, benzyl alcohol, and phenylethanol increased significantly. Treatment of reconstituted tobacco leaf concentrate with the enzyme solution resulted in prominent sweetness, a clean aftertaste, and improved aroma quality. Under the conditions of enzymatic hydrolysis temperature 40 ℃, shaking speed 150 r/min, pH value 7.0, and K⁺ concentration 10 mmol/L, the content of 3-oxo-α-ionol increased by 2.3 times. The genome of HZW-15 consists of 5 554 921 bp, encoding 76 glycoside hydrolase genes. 【Conclusion】 We identified the purified enzyme components by mass spectrometry, which indicated that 6-phospho-β-glucosidase, β-glucosidase, and 1,4-α-glucan branching enzyme are functional enzymes, providing a reference for the development of tobacco-used glycosidases.
- tobacco glycoside /
- glycoside-degrading bacteria /
- glycosidase /
- enzymatic property
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沈阳化工大学材料科学与工程学院沈阳 110142

烟草糖苷降解菌筛选及糖苷酶挖掘

作者简介: 冯颖杰(1990—),男,安徽省萧县人,河南中烟工业有限责任公司高级工程师,主要研究方向为烟草生物技术。E-mail:fyjwin123@163.com;

通讯作者: 黄申,huangshen@zzuli.edu.cn

Screening of tobacco glycoside-degrading bacteria and mining of glycosidases

Corresponding author: HUANG Shen, huangshen@zzuli.edu.cn

计量

通讯作者: 陈斌, bchen63@163.com

烟草糖苷降解菌筛选及糖苷酶挖掘

作者简介:冯颖杰(1990—),男,安徽省萧县人,河南中烟工业有限责任公司高级工程师,主要研究方向为烟草生物技术。E-mail:fyjwin123@163.com

通讯作者: 黄申, huangshen@zzuli.edu.cn

English Abstract

Screening of tobacco glycoside-degrading bacteria and mining of glycosidases

Corresponding author: HUANG Shen, huangshen@zzuli.edu.cn

目录

烟草糖苷降解菌筛选及糖苷酶挖掘

作者简介: 冯颖杰(1990—),男,安徽省萧县人,河南中烟工业有限责任公司高级工程师,主要研究方向为烟草生物技术。E-mail:fyjwin123@163.com;

通讯作者: 黄申,huangshen@zzuli.edu.cn

Screening of tobacco glycoside-degrading bacteria and mining of glycosidases

Corresponding author: HUANG Shen, huangshen@zzuli.edu.cn

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

烟草糖苷降解菌筛选及糖苷酶挖掘

作者简介:冯颖杰(1990—),男,安徽省萧县人,河南中烟工业有限责任公司高级工程师,主要研究方向为烟草生物技术。E-mail:fyjwin123@163.com

通讯作者: 黄申, huangshen@zzuli.edu.cn

English Abstract

Screening of tobacco glycoside-degrading bacteria and mining of glycosidases

Corresponding author: HUANG Shen, huangshen@zzuli.edu.cn

目录