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《轻工学报》严正声明

灵芝复合酶液关键酶特性分析及其在烟梗增香提质中的应用

张志平 ,  张翅 ,  苏怡馨 ,  张荣亚 ,  马亚萍 ,  张悦 ,  刘民昌 ,  文武

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张志平, 张翅, 苏怡馨, 等. 灵芝复合酶液关键酶特性分析及其在烟梗增香提质中的应用[J]. 轻工学报, 2026, 41(3): 120-131. doi: 10.12187/2026.03.012
引用本文: 张志平, 张翅, 苏怡馨, 等. 灵芝复合酶液关键酶特性分析及其在烟梗增香提质中的应用[J]. 轻工学报, 2026, 41(3): 120-131. doi: 10.12187/2026.03.012
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ZHANG Zhiping, ZHANG Chi, SU Yixin, et al. Characteristics of the key laccase in Ganoderma lucidum complex Enzyme liquid and its application in aroma enhancement and quality improvement of tobacco stems[J]. Journal of Light Industry, 2026, 41(3): 120-131. doi: 10.12187/2026.03.012
Citation: ZHANG Zhiping, ZHANG Chi, SU Yixin, et al. Characteristics of the key laccase in Ganoderma lucidum complex Enzyme liquid and its application in aroma enhancement and quality improvement of tobacco stems[J]. Journal of Light Industry, 2026, 41(3): 120-131. doi: 10.12187/2026.03.012
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灵芝复合酶液关键酶特性分析及其在烟梗增香提质中的应用

  • 1. 郑州轻工业大学 食品与生物工程学院, 河南 郑州 450001;

  • 2. 四川中烟工业有限责任公司 技术中心, 四川 成都 610066

    • 作者简介: 张志平(1981—),男,河南省安阳市人,郑州轻工业大学副教授,博士,主要研究方向为生物质资源化利用。E-mail:zzp@zzuli.edu.cn;
    通讯作者: 刘民昌,liumc@sctobacco.com

  • 基金项目: 河南省重点研发专项项目(241111110400)
    四川中烟工业有限责任公司科技项目(10202313BA510)

  • 中图分类号: TS49

Characteristics of the key laccase in Ganoderma lucidum complex Enzyme liquid and its application in aroma enhancement and quality improvement of tobacco stems

  • 1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;

  • 2. Technology Center, China Tobacco Sichuan Industrial Co., Ltd., Chengdu 610066, China

    • Corresponding author: LIU Minchang, liumc@sctobacco.com
  • Received Date: 2024-06-18
    Accepted Date: 2025-07-31

    CLC number: TS49

  • 摘要: 【目的】 明确灵芝复合酶液的关键生物酶及其酶学特性,以及发酵烟梗的增香提质机制。【方法】 通过非变性聚丙烯酰胺凝胶电泳和酶活力测试确定灵芝复合酶液的关键生物酶,采用酶分离纯化技术和分子结构模拟研究关键酶的酶学特性和结构特征,进一步通过扫描电子显微镜和GC-MS分析明确灵芝复合酶液处理烟梗的增香提质效果。【结果】 灵芝复合酶液具有多种酶活力,其中漆酶活力最高,达35 466.67 U/L,并含3种漆酶同工酶;关键漆酶Lac3为三域结构漆酶,相对分子质量为58.4 kDa,含500个氨基酸,最适温度、pH值分别为55 ℃和3.0,1 mmol/L Cu2+对其活力具有促进作用,而Fe3+的抑制作用最大;经灵芝复合酶液酶解后,烟梗致密结构被破坏,木质素降解率达22.56%;卷烟加香感官评价得分明显提升,梗丝中醛类、酮类、酚类等共计17种香气成分总量提升,实现烟梗增香提质目的。【结论】 灵芝复合酶液以漆酶为关键酶,可降解梗丝木质素、提升香气,助力资源化利用。
    Abstract: 【Objective】 This study aimed to identify the key enzyme in Ganoderma lucidum complex enzyme liquid and its enzymatic characteristics, and to elucidate the mechanism of aroma enhancement and quality improvement of tobacco stems by fermentation. 【Methods】 The key enzyme in Ganoderma lucidum complex enzyme liquid was identified by native polyacrylamide gel electrophoresis (native-PAGE) and enzymatic activity assays. Enzyme separation and purification combined with molecular structure modeling were employed to investigate the enzymatic properties and structural features of the key enzyme. Scanning electron microscopy (SEM) and GC-MS analysis were then used to evaluate the aroma-enhancing and quality-improving effects of the complex enzyme liquid on tobacco stems. 【Results】 The Ganoderma lucidum complex enzyme liquid exhibited multiple enzymatic activities, among which laccase activity was the highest at 35 466.67 U/L, and three laccase isoenzymes were identified. The key laccase, Lac3, was a three-domain laccase with a molecular weight of 58.4 kDa and 500 amino acids. Its optimal temperature and pH were 55 ℃ and 3.0, respectively. Cu2+ at 1 mmol/L promoted its activity, while Fe3+ exerted the strongest inhibitory effect. After enzymatic treatment with the complex enzyme liquid, the compact structure of tobacco stem shreds was disrupted, and the lignin degradation rate reached 22.56%. Sensory evaluation scores were significantly improved. Compared with the control, the total content of 17 aroma compounds, including aldehydes, ketones, and phenols, in the stem shreds increased, thus achieving the goal of aroma enhancement and quality improvement of tobacco stems. 【Conclusion】 Ganoderma lucidum complex enzyme liquid, with laccase as the key enzyme, can degrade lignin in tobacco stem shreds and enhance aroma, thereby promoting resource utilization.
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  • 通讯作者:  刘民昌, liumc@sctobacco.com
  • 收稿日期:  2024-06-18
  • 修回日期:  2025-07-31
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张志平, 张翅, 苏怡馨, 等. 灵芝复合酶液关键酶特性分析及其在烟梗增香提质中的应用[J]. 轻工学报, 2026, 41(3): 120-131. doi: 10.12187/2026.03.012
引用本文: 张志平, 张翅, 苏怡馨, 等. 灵芝复合酶液关键酶特性分析及其在烟梗增香提质中的应用[J]. 轻工学报, 2026, 41(3): 120-131. doi: 10.12187/2026.03.012
shu
ZHANG Zhiping, ZHANG Chi, SU Yixin, et al. Characteristics of the key laccase in Ganoderma lucidum complex Enzyme liquid and its application in aroma enhancement and quality improvement of tobacco stems[J]. Journal of Light Industry, 2026, 41(3): 120-131. doi: 10.12187/2026.03.012
Citation: ZHANG Zhiping, ZHANG Chi, SU Yixin, et al. Characteristics of the key laccase in Ganoderma lucidum complex Enzyme liquid and its application in aroma enhancement and quality improvement of tobacco stems[J]. Journal of Light Industry, 2026, 41(3): 120-131. doi: 10.12187/2026.03.012
shu

灵芝复合酶液关键酶特性分析及其在烟梗增香提质中的应用

    作者简介:张志平(1981—),男,河南省安阳市人,郑州轻工业大学副教授,博士,主要研究方向为生物质资源化利用。E-mail:zzp@zzuli.edu.cn
    通讯作者: 刘民昌, liumc@sctobacco.com
  • 1. 郑州轻工业大学 食品与生物工程学院, 河南 郑州 450001;
  • 2. 四川中烟工业有限责任公司 技术中心, 四川 成都 610066
  • 收稿日期: 2024-06-18
  • 录用日期: 2025-07-31
基金项目:  河南省重点研发专项项目(241111110400)四川中烟工业有限责任公司科技项目(10202313BA510)

摘要: 【目的】 明确灵芝复合酶液的关键生物酶及其酶学特性,以及发酵烟梗的增香提质机制。【方法】 通过非变性聚丙烯酰胺凝胶电泳和酶活力测试确定灵芝复合酶液的关键生物酶,采用酶分离纯化技术和分子结构模拟研究关键酶的酶学特性和结构特征,进一步通过扫描电子显微镜和GC-MS分析明确灵芝复合酶液处理烟梗的增香提质效果。【结果】 灵芝复合酶液具有多种酶活力,其中漆酶活力最高,达35 466.67 U/L,并含3种漆酶同工酶;关键漆酶Lac3为三域结构漆酶,相对分子质量为58.4 kDa,含500个氨基酸,最适温度、pH值分别为55 ℃和3.0,1 mmol/L Cu2+对其活力具有促进作用,而Fe3+的抑制作用最大;经灵芝复合酶液酶解后,烟梗致密结构被破坏,木质素降解率达22.56%;卷烟加香感官评价得分明显提升,梗丝中醛类、酮类、酚类等共计17种香气成分总量提升,实现烟梗增香提质目的。【结论】 灵芝复合酶液以漆酶为关键酶,可降解梗丝木质素、提升香气,助力资源化利用。

English Abstract

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