蛹虫草双向发酵组合物的抗氧化、抗皱紧致及光老化修复功效评价
Evaluation of the antioxidant,anti-wrinkle／skin-firming and photoaging repair activities of Cordyceps militaris bidirectional fermentation combinations
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摘要: 【目的】 开发蛹虫草组合发酵产品并探索珍稀药材高效利用的新路径。【方法】 采用液态发酵法结合中药-菌种双向发酵技术制备蛹虫草单菌及分别与甘草、人参茎叶、三七茎叶、铁皮石斛组合的5种发酵液,通过DPPH和ABTS+自由基清除实验和弹性蛋白酶抑制实验,结合UVB诱导的人包皮成纤维细胞(HFF)光老化模型和裸鼠皮肤光老化模型,评价发酵液体外抗氧化、抗皱紧致及光老化修复能力。【结果】 蛹虫草-甘草发酵液(Y2)的DPPH和ABTS+自由基清除率及弹性蛋白酶抑制率均显著优于其他发酵液;该发酵液可显著上调UVB损伤HFF细胞中Ⅰ型胶原蛋白(COL-Ⅰ)合成,下调基质金属蛋白酶1(MMP-1)分泌;在裸鼠皮肤光老化模型中,Y2能减轻皮肤组织病理损伤,改善胶原纤维排列。【结论】 蛹虫草-甘草发酵液兼具良好的抗氧化与光老化修复活性,可为蛹虫草功能产品开发及珍稀药材的高效利用提供新方向。Abstract: 【Objective】 To develop fermented combinatorial products of Cordyceps militaris and explore a novel strategy for the efficient utilization of rare Chinese medicinal herbs.【Methods】 Five fermentation broths were prepared by submerged liquid fermentation combined with the bidirectional fermentation technique of Chinese herbal medicines and fungal strains. These included a C.militaris monoculture broth and its combinatorial broths with Glycyrrhiza uralensis, Panax ginseng stems and leaves, Panax notoginseng stems and leaves, and Dendrobium officinale. The antioxidant, anti-wrinkle/firming, and photoaging repair capacities of these broths were evaluated using DPPH and ABTS+ free scavenging assays, an elastase inhibition assay, a UVB-induced human skin fibroblast (HFF) photoaging model, and a nude mouse skin photoaging model.【Results】 The C.militaris-G.uralensis fermentation broth (Y2) significantly outperformed the other broths in DPPH and ABTS+ free scavenging activities and elastase inhibition rate. This broth significantly upregulated the synthesis of type Ⅰ collagen (COL-Ⅰ) and downregulated the secretion of matrix metalloproteinase-1 (MMP-1) in UVB-damaged HFF cells. In the nude mouse skin photoaging model, Y2 alleviated skin tissue pathological damage and improved the arrangement of collagen fibers.【Conclusion】 The C.militaris-G.uralensis fermentation broth exhibits excellent antioxidant and photoaging repair activities, thus providing a new direction for the development of functional C.militaris products and the efficient utilization of rare medicinal herbs.
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[1]
臧运芳,李越,金锋,等.蛹虫草生物活性成分及其生理功能的研究进展[J].食品研究与开发,2025,46(13):211-217.
ZANG Y F,LI Y,JIN F,et al.Advances in research on bioactive components and physiological functions of Cordyceps militaris[J].Food Research and Development,2025,46(13):211-217. -
[2]
左锦辉,贡晓燕,董银卯,等.蛹虫草的活性成分和药理作用及其应用研究进展[J].食品科学,2018,39(21):330-339.
ZUO J H,GONG X Y,DONG Y M,et al.Research achievements in bioactive components,pharmacological effects and applications of Cordyceps militaris[J].Food Science,2018,39(21):330-339. -
[3]
童奇伟,周吉亮,罗珊珊,等.响应面法优化蛹虫草液体发酵配方及条件的研究[J].食药用菌,2024,32(1):52-59.
TONG Q W,ZHOU J L,LUO S S,et al.Optimization of formula and conditions for liquid fermentation of Cordyceps militaris by response surface method[J].Edible and Medicinal Mushrooms,2024,32(1):52-59. -
[4]
李丽.珍稀中草药铁皮石斛和蛹虫草提取物护肤品的开发研究[D].杭州:浙江理工大学,2023. Li L.Development research of skincare products with extracts from rare Chinese medicinal herbs Dendrobium officinale and Cordyceps militaris[D].Hangzhou:Zhejiang Sci-Tech University,2023.
-
[5]
PAPACCIO F,D'ARINO A,CAPUTO S,et al.Focus on the contribution of oxidative stress in skin aging[J].Antioxidants,2022,11(6):1121.
-
[6]
GAO F,SUN Y J,GAN H H.Atractylodin mitigates UVB radiation-induced oxidative stress and photoaging responses by enhancing NrF2 signaling in human epidermal keratinocytes[J].Archives of Dermatological Research,2024,317(1):160.
-
[7]
LEI X X.Mechanisms and therapeutic roles of medicinal plants in skin photoaging[J].Clinical,Cosmetic and Investigational Dermatology,2025,18:1709-1726.
-
[8]
KALTCHENKO M V,CHIEN A L.Photoaging:current concepts on molecular mechanisms,prevention,and treatment[J].American Journal of Clinical Dermatology,2025,26(3):321-344.
-
[9]
LEPHART E D.Skin aging and oxidative stress:equol’s anti-aging effects via biochemical and molecular mechanisms[J].Ageing Research Reviews,2016,31:36-54.
-
[10]
吴瑞麒,窦博,马小来,等.发酵技术在化妆品原料生产中的应用及研究进展[J].发酵科技通讯,2025,54(3):142-146.
WU R Q,DOU B,MA X L,et al.Research progress of fermentation technology applied to plant raw materials in cosmetics[J].Bulletin of Fermentation Science and Technology,2025,54(3):142-146. -
[11]
徐晨,苏娟.生物技术在中药制药工程中的应用研究[J].产业创新研究,2025(18):87-89. XU C,SU J.Application of biotechnology in pharmaceutical engineering of traditional Chinese medicine[J].Industrial Innovation,2025
(18):87-89. -
[12]
薛燕,李丽,刘光荣,等.铁皮石斛多糖液态发酵工艺及其抗氧化活性研究[J].食品工业科技,2017,38(9):155-159.
XUE Y,LI L,LIU G R,et al.Study on polysaccharides submerged fermentation from Dendrobium officinale and its antioxidant activity[J].Science and Technology of Food Industry,2017,38(9):155-159. -
[13]
秦令祥,丁昱婵,赵俊芳,等.蛹虫草多糖提取工艺优化及其抗菌、抗氧化活性[J].食品研究与开发,2023,44(11):159-165.
QIN L X,DING Y C,ZHAO J F,et al.Optimization of extraction process of Cordyceps militaris polysaccharides and its antibacterial and antioxidant activities[J].Food Research and Development,2023,44(11):159-165. -
[14]
CHEN J J,LIU Y,ZHAO Z,et al.Oxidative stress in the skin:Impact and related protection[J].International Journal of Cosmetic Science,2021,43(5):495-509.
-
[15]
KIM E,TOLLENAERE M,SENNELIER B,et al.Analysis of active components and transcriptome of Freesia refracta callus extract and its effects against oxidative stress and wrinkles in skin[J].International Journal of Molecular Sciences,2024,25(15):8150.
-
[16]
WANG G X,FEI W C,ZHI L L,et al.Fermented tea leave extract against oxidative stress and ageing of skin in vitro and in vivo[J].International Journal of Cosmetic Science,2025,47(1):1-17.
-
[17]
KUCHARSKA E,ZAGÓRSKA-DZIOK M,BILEWICZ P,et al.Use of Syzygium aromaticum L.fermented plant extract to enhance antioxidant potential:fermentation kinetics[J].Applied Sciences,2024,14(11):4900.
-
[18]
王玲,王刚,廖知恒,等.大米多肽在抗皱/修护护肤品中的应用及功效[J].香料香精化妆品,2024(2):112-119. WANG L,WANG G,LIAO Z H,et al.Application and efficacy of rice polypeptides in anti wrinkle/repair skincare products[J].Flavour Fragrance Cosmetics,2024
(2):112-119. -
[19]
VANJARE B D,SEOK EOM Y,RAZA H,et al.Elastase inhibitory activity of quinoline Analogues:synthesis,kinetic mechanism,cytotoxicity,chemoinformatics and molecular docking studies[J].Bioorganic & Medicinal Chemistry,2022,63:116745.
-
[20]
ZHAO Y S,EWEYS A S,ZHANG J Y,et al.Fermentation affects the antioxidant activity of plant-based food material through the release and production of bioactive components[J].Antioxidants,2021,10(12):2004.
-
[21]
胡楚涓,王璐璐,蒋建东,等.天然活性成分抑制皮肤胶原蛋白流失的研究进展[J].药学学报,2025,60(2):269-279.
HU C J,WANG L L,JIANG J D,et al.Research progress of natural bioactive products in resisting loss of skin collagen[J].Acta Pharmaceutica Sinica,2025,60(2):269-279. -
[22]
LIU S W,YOU L,ZHAO Y X,et al.Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and type I procollagen production in mice[J].Journal of Agricultural and Food Chemistry,2018,66(32):8537-8546.
-
[23]
KIM K J,XUAN S H,PARK S N.Licoricidin,an isoflavonoid isolated from Glycyrrhiza uralensis fisher,prevents UVA-induced photoaging of human dermal fibroblasts[J].International Journal of Cosmetic Science,2017,39(2):133-140.
-
[24]
LEE Y R,NOH E M,JEONG E Y,et al.Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-κB pathway in human dermal fibroblasts[J].Experimental & Molecular Medicine,2009,41(8):548-554.
-
[25]
LEE H Y,KIM E J,CHO D Y,et al.Photoprotective effect of fermented and aged mountain-cultivated ginseng sprout (Panax ginseng)on ultraviolet radiation-induced skin aging in a hairless mouse model[J].Nutrients,2023,15(7):1715.
-
[26]
KRUTMANN J,SCHALKA S,WATSON R E B,et al.Daily photoprotection to prevent photoaging[J].Photodermatology,Photoimmunology & Photomedicine,2021,37(6):482-489.
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