毛蜂窝菌粗多糖超声波辅助提取工艺优化及其体外生物活性研究
Optimization of ultrasonic-assisted extraction process and in vitro biological activities of crude polysaccharides from Hexagonia apiaria
-
摘要: 【目的】优化毛蜂窝菌粗多糖(Hexagonia apiaria Polysaccharide,HAP)的超声波辅助提取工艺,探究HAP多维体外生物活性特征,拓宽其在功能性食品与医药保健领域的应用范围。【方法】以毛蜂窝菌为原料,采用超声波辅助法提取HAP,利用单因素和响应面试验优化提取工艺,通过自由基清除、α-葡萄糖苷酶抑制及巨噬细胞实验评价HAP体外生物活性。【结果】 HAP最佳提取工艺条件为超声功率320 W、料液比1∶15.5、提取温度85.5 ℃、提取时间56 min,在此条件下,HAP得率为(1.91±0.02)%; 1 mg/mL HAP的DPPH和ABTS+自由基清除率分别为(51.70±2.50)%和(78.51±1.42)%,2 mg/mL HAP的α-葡萄糖苷酶抑制率为(61.99±1.45)%,并呈现浓度依赖效应;25 μg/mL HAP对小鼠单核巨噬细胞系RAW264.7增殖具有抑制作用,细胞相对增殖率为(77.28±4.73)%,且该质量浓度HAP可促进RAW264.7细胞释放NO,释放量为(24.05±1.02) μmol/L,显著高于阳性对照组((12.52±0.69) μmol/L),具有一定的免疫刺激潜力。【结论】HAP具有较好的体外抗氧化、降血糖及免疫刺激活性,具备作为功能性食品或医药保健成分的开发潜力。Abstract: 【Objective】This study aimed to optimize the ultrasound-assisted extraction process of crude Hexagonia apiaria polysaccharide (HAP) and to investigate its multiple in vitro biological activities, thereby broadening its potential applications in functional foods and healthcare. 【Methods】Hexagonia apiaria was used as the raw material, and HAP was extracted by ultrasound-assisted extraction. The extraction process was optimized using single-factor experiments and response surface methodology. The in vitro biological activities of HAP were evaluated by radical scavenging, α-glucosidase inhibition, and macrophage assays.【Results】The optimal extraction conditions were as follows: ultrasonic power, 320 W; solid-to-liquid ratio, 1∶15.5; extraction temperature, 85.5 ℃; and extraction time, 56 min. Under these conditions, the HAP yield was (1.91±0.02)%. At 1 mg/mL, the DPPH and ABTS+ radical scavenging rates of HAP were (51.70±2.50)% and (78.51±1.42)%, respectively. At 2 mg/mL, the α-glucosidase inhibition rate of HAP reached (61.99±1.45)%, showing a concentration-dependent effect. At a mass concentration of 25 μg/mL, HAP inhibited the proliferation of the murine monocyte/macrophage cell line RAW264.7, with a relative cell proliferation rate of (77.28±4.73)%. Interestingly, at this concentration, HAP significantly promoted NO release from RAW264.7, with an NO release of (24.05±1.02) μmol/L, which was significantly higher than that in the positive control group ((12.52±0.69) μmol/L), indicating immunosuppression potential. 【Conclusion】HAP exhibits promising in vitro antioxidant, hypoglycemic, and immunostimulatory activities in a concentration-dependent manner, suggesting its potential for development as a functional food or nutraceutical/pharmaceutical ingredient.
-
-
[1]
毕志树,郑国杨,李泰辉.广东大型真菌志[M].广州:广东科技出版社,1994:281-282. Bi Z S,Zheng G Y,Li T H.Flora of large fungi in Guangdong [M].Guangzhou:Guangdong Science and Technology Press,1994:281
-282. -
[2]
孟翔鹏,马琳.食药用真菌的研究进展及其应用前景展望[J].中国现代中药,2009,11(10):7-10.
Meng X P,Ma L.Research progress and application prospect of edible and medicinal fungi[J].Modern Chinese Medicine,2009,11(10):7-10. -
[3]
翁梁,温鲁.药用真菌多糖研究进展[J].食品科学,2008,29(12):748-751.
Weng L,Wen L.Review of research on medicinal fungi polysaccharides[J].Food Science,2008,29(12):748-751. -
[4]
李浩华,陈玉婵,陶美华,等.毛蜂窝菌提取物体外抗肿瘤活性的研究[J].食用菌学报,2009,16(3):57-60.
Li H H,Chen Y C,Tao M H,et al.In vitro anti-tumor activities of hexagona apiaria extracts[J].Acta Edulis Fungi,2009,16(3):57-60. -
[5]
李浩华,章卫民,潘清灵,等.药用真菌毛蜂窝菌的栽培技术研究初报[C]//中国菌物学会,湖北省科学技术厅,湖北省科学技术协会.中国菌物学会第四届会员代表大会暨全国第七届菌物学学术讨论会论文集:2008年卷.广州:广东省微生物研究所,2008:363-366. Li H H,Zhang W M,Pan Q L,et al.Preliminary report on cultivation techniques for the medicinal fungus Hexagonia apiaria [C]//Chinese Mycological Society,Hubei Provincial Department of Science and Technology,Hubei Provincial Association for Science and Technology.Proceedings of the fourth general assembly of members of the Chinese mycological society and the seventh national mycological symposium:2008 Volume.Guangzhou:Guangdong Institute of Microbiology,2008
:363-366. -
[6]
李浩华,章卫民,潘清灵,等.毛蜂窝菌的栽培技术研究[J].食用菌学报,2008,15(3):31-34.
Li H H,Zhang W M,Pan Q L,et al.Cultivation of the medicinal fungus,Hexagonia apiaria[J].Acta Edulis Fungi,2008,15(3):31-34. -
[7]
彭毓敏,李皓,朱峰,等.毛蜂窝菌菌油化学成分分析与DPPH自由基清除活性[J].佛山科学技术学院学报(自然科学版),2024,42(2):74-80.
Peng Y M,Li H,Zhu F,et al.Chemical compositions analysis and DPPH radical scavenging activity of fungus oil of Hexagonia apiaria Pers.:Fr[J].Journal of Foshan University (Natural Science Edition),2024,42(2):74-80. -
[8]
张璐,张斌,易延逵.毛蜂窝菌多糖提取工艺优化[J].辽宁中医药大学学报,2017,19(11):62-64.
Zhang L,Zhang B,Yi Y K.Extraction technological optimization of polysaccharides in Hexagona apiaria[J].Journal of Liaoning University of Traditional Chinese Medicine,2017,19(11):62-64. -
[9]
Schritt H,Vidi S,Pleissner D.Spent mushroom substrate and sawdust to produce Mycelium-based thermal insulation composites[J].Journal of Cleaner Production,2021,313:127910.
-
[10]
李兴海,邵建富,刘仕杰,等.猴头菇多糖联合转化生长因子-β1对胃癌细胞增殖及微小RNA-302a的影响[J].肿瘤代谢与营养电子杂志,2023,10(3):408-414.
Li X H,Shao J F,Liu S J,et al.Hericium erinaceus polysaccharide combined with different doses of TGF-β1 Effects of miR-302a on the proliferation of gastric cancer cells[J].Electronic Journal of Metabolism and Nutrition of Cancer,2023,10(3):408-414. -
[11]
谭敏颖,戴川景,卢学敏,等.银耳多糖对人软骨细胞的增殖效应和抗炎作用[J].食品工业科技,2024,45(1):1-8.
Tan M Y,Dai C J,Lu X M,et al.Proliferation and anti-inflammatory effects of Tremella fuciformis polysaccharide on human chondrocytes[J].Science and Technology of Food Industry,2024,45(1):1-8. -
[12]
Zhang Y W,Feng Y B,Wang W S,et al.Characterization and hepatoprotections of Ganoderma lucidum polysaccharides against multiple organ dysfunction syndrome in mice[J].Oxidative Medicine and Cellular Longevity,2021,2021:9703682.
-
[13]
Zheng C Q,Shao Y W,Hao L M,et al.Extraction,characterization,and biological activities of a polysaccharide from Poria cocos peels[J].Journal of Food Processing and Preservation,2022,46(10):e16821.
-
[14]
姚艳婷,杨小兵,陈旭洁,等.富硒蛹虫草多糖的结构表征及体外免疫调节活性[J].食品科学,2023,44(22):39-48.
Yao Y T,Yang X B,Chen X J,et al.Structural characterization and in vitro immunomodulatory activity of polysaccharides from selenium-enriched Cordyceps militaris[J].Food Science,2023,44(22):39-48. -
[15]
刘楠楠.黑木耳多糖的提取纯化、结构表征及降糖活性研究[D].西安:陕西科技大学,2022. Liu N N.Extraction,purification,structural characterization,and hypoglycemic activity of polysaccharides from Black Fungus [D].Xi’an:Shaanxi University of Science and Technology,2022.
-
[16]
吴睿婷,付王威,万敏,等.黑灵芝多糖对糖尿病大鼠血糖血脂调节及肠道菌群的影响[J].食品科学,2022,43(5):91-102.
Wu R T,Fu W W,Wan M,et al.Effect of polysaccharide from Ganoderma atrum on hyperglycemia,blood lipid and gut microbiota of diabetic rats[J].Food Science,2022,43(5):91-102. -
[17]
张郡月,张瑞,柴秋泉,等.灵芝多糖的超声辅助提取工艺优化及抗氧化活性分析[J].现代食品科技,2026,42(4):184-194.
Zhang J Y,Zhang R,Chai Q Q,et al.Optimization of ultrasound-assisted extraction process and antioxidant activity of Ganoderma lucidum polysaccharides[J].Modern Food Science & Technology,2026,42(4):184-194. -
[18]
原梦瑶,康淑荷,崔璐娟,等.红芪多糖超声提取工艺优化及抗氧化、美白和吸湿保湿活性[J].精细化工,2025,42(3):577-586.
Yuan M Y,Kang S H,Cui L J,et al.Optimization of ultrasonic extraction process for polysaccharides from Radix Hedysari and their antioxidant,whitening,moisture absorption and moisturizing activities[J].Fine Chemicals,2025,42(3):577-586. -
[19]
沈琴,路乐乐,潘票,等.黑木耳硒化多糖制备、结构及体外活性分析[J].食品工业科技,2025,46(10):250-259.
Shen Q,Lu L L,Pan P,et al.Preparation,structure andin vitro activity analysis of selenopolysaccharide from Auricularia[J].Science and Technology of Food Industry,2025,46(10):250-259. -
[20]
张凤国,张永勤,邢明霞,等.Lowrry法测定蛋白浓度的最佳波长选择[J].青岛科技大学学报(自然科学版),2021,42(2):30-36.
Zhang F G,Zhang Y Q,Xing M X,et al.Selection of optimal detection wavelength for determinaiton of protein concentration by Lowrry method[J].Journal of Qingdao University of Science and Technology (Natural Science Edition),2021,42(2):30-36. -
[21]
高若涵,马楠,杨明哲,等.燕麦非淀粉多糖发酵富集法工艺优化及其免疫刺激活性分析[J].食品工业科技,2025,46(10):21-32.
Gao R H,Ma N,Yang M Z,et al.Optimization of the fermentation enrichment method for oat non-starch polysaccharides and study of their immunostimulatory activity[J].Science and Technology of Food Industry,2025,46(10):21-32. -
[22]
Niu G G,You G,Zhou X Y,et al.Physicochemical properties and in vitro hypoglycemic activities of hsian-tsao polysaccharide fractions by gradient ethanol precipitation method[J].International Journal of Biological Macromolecules,2023,231:123274.
-
[23]
王亮亮.川芎茎叶可溶性多糖提取、结构表征及生物活性研究[D].成都:成都大学,2025. Wang L L.Soluble polysaccharide extraction,structural characterization,and bioactivity study of ligusticum chuanxiong rhizome and leaves [D].Chengdu:Chengdu University,2025.
-
[24]
Yelithao K,Surayot U,Lee C,et al.Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune[J].Carbohydrate Polymers,2019,218:37-45.
-
[25]
刘永玲,刘向炼,陶欣,等.八月瓜果皮多糖提取工艺优化及其体外抗氧化、降糖活性分析[J].食品工业科技,2024,45(19):17-24.
Liu Y L,Liu X L,Tao X,et al.Optimization of polysaccharides extraction from the peels of Akebia trifoliate and evaluation of its in vitro antioxidant and hypoglycemic activity[J].Science and Technology of Food Industry,2024,45(19):17-24. -
[26]
陈蕾,郑甜甜,朱贺宇,等.响应面法优化超声辅助提取姬菇多糖及其体外抗氧化性[J].中国食品添加剂,2024,35(1):143-149.
Chen L,Zheng T T,Zhu H Y,et al.Optimization of ultrasound-assisted extraction of polysaccharides from Agaricus blazei by response surface methodology and its in vitro antioxidant activity[J].China Food Additives,2024,35(1):143-149. -
[27]
Gu J Y,Zhang H H,Zhang J X,et al.Optimization,characterization,rheological study and immune activities of polysaccharide from Sagittaria sagittifolia L[J].Carbohydrate Polymers,2020,246:116595.
-
[28]
Chen Y,Xu M,Wang X Y,et al.Preparation of wood ear medicinal mushroom,Auricularia auricula-judae (Agaricomycetes),melanin and its antioxidant properties:evaluation in vitro and in vivo[J].International Journal of Medicinal Mushrooms,2021,23(6):89-100.
-
[29]
曾祥玉.松塔鳞片多糖的提取及其免疫生理活性研究[D].长春:吉林农业大学,2021. Zeng X Y.Extraction of polysaccharides from pine cone scales and investigation of their immunophysiological activity [D].Changchun:Jilin Agricultural University,2021.
-
[30]
李文宇,王茜,沈静,等.超声波辅助提取新疆香梨多糖的工艺研究[J].食品研究与开发,2023,44(4):136-142.
Li W Y,Wang Q,Shen J,et al.Study on ultrasound-assisted extraction of polysaccharides from fragrant pears in Xinjiang[J].Food Research and Development,2023,44(4):136-142. -
[31]
余金岂,邬柯奕,陈正睿,等.珊瑚状猴头菌多糖超声辅助提取工艺优化及抗结直肠癌活性研究[J].生物技术通报,2024,40(11):78-87.
Yu J Q,Wu K Y,Chen Z R,et al.Study on the optimization of ultrasonic-assisted extraction process of Hericium coralloides (scop.)pers.polysaccharides and its anti-colorectal cancer activity[J].Biotechnology Bulletin,2024,40(11):78-87. -
[32]
黄明浩,黄泰奇,邓丽娟.响应面法优化白英粗多糖提取工艺及其体外抗氧化活性的分析[J].食品工业科技,2023,44(22):219-225.
Huang M H,Huang T Q,Deng L J.Optimization of Solanum lyratum crude polysaccharide extraction process using response surface methodology and analysis of its in vitro antioxidant activity[J].Science and Technology of Food Industry,2023,44(22):219-225. -
[33]
李良玉,李朝阳,宋大巍,等.响应面法优化梨小豆粗多糖超声波提取工艺研究[J].天然产物研究与开发,2016,28(10):1590-1596
,1516. Li L Y,Li C Y,Song D W,et al.Optimization of ultrasonic extraction of crude polysaccharide from pear adzuki beans[J].Natural Product Research and Development,2016,28(10):1590-1596,1516. -
[34]
柴轶涛,胡情,柳冬梅,等.源于中药的α-葡萄糖苷酶抑制剂研究进展[J].中国现代应用药学,2025,42(11):1967-1984.
Chai Y T,Hu Q,Liu D M,et al.Research progress of α-glucosidase inhibitors from Chinese herbs[J].Chinese Journal of Modern Applied Pharmacy,2025,42(11):1967-1984. -
[35]
Jha R,Goyal K,Mehan S,et al.Dual α-amylase and α-glucosidase inhibitors:recent progress from natural and synthetic resources[J].Bioorganic Chemistry,2025,163:108762.
-
[36]
冯学珍,覃慧逢,冯书珍.网地藻多糖对α-葡萄糖苷酶体外抑制作用的研究[J].食品研究与开发,2020,41(3):35-40.
Feng X Z,Qin H F,Feng S Z.Study on inhibition kinetics of polysaccharide from Dictyota dichotoma on α-glucosidase[J].Food Research and Development,2020,41(3):35-40. -
[37]
方嘉沁,张潼,符树勇,等.不同分子量余甘子多糖理化性质及其对α-葡萄糖苷酶的抑制活性比较[J].现代食品科技,2025,41(11):144-152.
Fang J Q,Zhang T,Fu S Y,et al.Comparison on the physicochemical characteristics and inhibitory activity against α-glucosidase of the polysaccharides with different molecular weights from Phyllanthus emblica L[J].Modern Food Science and Technology,2025,41(11):144-152.
-
[1]
-
点击查看大图
计量
- PDF下载量: 19
- 文章访问数: 274
- 引证文献数: 0
下载:
