毛蜂窝菌粗多糖超声波辅助提取工艺优化及其体外生物活性研究

李良玉; 张思影; 李雪静; 曹荣安; 王坤; 王菲菲

doi:10.12187/2026.05.001

October 2026

Article Contents

Li Liangyu, Zhang Siying, Li Xuejing, et al. Optimization of ultrasonic-assisted extraction process and in vitro biological activities of crude polysaccharides from Hexagonia apiaria[J]. Journal of Light Industry, 2026, 41(5): 1-12. doi: 10.12187/2026.05.001

Citation: Li Liangyu, Zhang Siying, Li Xuejing, et al. Optimization of ultrasonic-assisted extraction process and in vitro biological activities of crude polysaccharides from Hexagonia apiaria[J]. Journal of Light Industry, 2026, 41(5): 1-12. doi: 10.12187/2026.05.001 shu

Optimization of ultrasonic-assisted extraction process and in vitro biological activities of crude polysaccharides from Hexagonia apiaria

Li Liangyu ^1,2 ,
Zhang Siying ³ ,
Li Xuejing ³ ,
Cao Rongan ³ ,
Wang Kun ³ ,
Wang Feifei ⁴

1. National Engineering Research Center for Minor Cereals, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2. Engineering Research Center of Processing and Utilization of Grain By-products, Ministry of Education, Daqing 163319, China;
3. College of Food, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
4. Department of Food and Pharmaceutical Engineering, Heilongjiang Agricultural Economy Vocational College, Mudanjiang 157000, China

Received Date: 2025-11-11
Accepted Date: 2026-01-05

Abstract

【Objective】This study aimed to optimize the ultrasound-assisted extraction process of crude Hexagonia apiaria polysaccharide (HAP) and to investigate its multiple in vitro biological activities, thereby broadening its potential applications in functional foods and healthcare. 【Methods】Hexagonia apiaria was used as the raw material, and HAP was extracted by ultrasound-assisted extraction. The extraction process was optimized using single-factor experiments and response surface methodology. The in vitro biological activities of HAP were evaluated by radical scavenging, α-glucosidase inhibition, and macrophage assays.【Results】The optimal extraction conditions were as follows: ultrasonic power, 320 W; solid-to-liquid ratio, 1∶15.5; extraction temperature, 85.5 ℃; and extraction time, 56 min. Under these conditions, the HAP yield was (1.91±0.02)%. At 1 mg/mL, the DPPH and ABTS⁺ radical scavenging rates of HAP were (51.70±2.50)% and (78.51±1.42)%, respectively. At 2 mg/mL, the α-glucosidase inhibition rate of HAP reached (61.99±1.45)%, showing a concentration-dependent effect. At a mass concentration of 25 μg/mL, HAP inhibited the proliferation of the murine monocyte/macrophage cell line RAW264.7, with a relative cell proliferation rate of (77.28±4.73)%. Interestingly, at this concentration, HAP significantly promoted NO release from RAW264.7, with an NO release of (24.05±1.02) μmol/L, which was significantly higher than that in the positive control group ((12.52±0.69) μmol/L), indicating immunosuppression potential. 【Conclusion】HAP exhibits promising in vitro antioxidant, hypoglycemic, and immunostimulatory activities in a concentration-dependent manner, suggesting its potential for development as a functional food or nutraceutical/pharmaceutical ingredient.
- Hexagonia apiaria,
- polysaccharide,
- ultrasound-assisted extraction,
- process optimization,
- in vitro biological activity
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Optimization of ultrasonic-assisted extraction process and in vitro biological activities of crude polysaccharides from Hexagonia apiaria

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